Data/hora: 23/09/2024 16:15:14

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	14/10/2009	
Data da última atualização: 	02/02/2010	
Autoria: 	GARCIA, S. A. L.; TALEBI, R.; FERREIRA, C. F.; VROH, I.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J.	
Afiliação: 	S. A. L. Garcia, PRI/UFLA; R.T. ALEBI, PRI-Plant Research International; Cláudia Fortes Ferreira, CNPMF; I. VROH, IITA; Luciano Vilela Paiva, UFL; Manoel Teixeira Souza Junior, LABEX Europe; Gert H. J. Kema, PRI-Plant Research International.	
Título: 	Identification and validation of EST-derived Molecular markers, TRAP and VNTR, for banana research.	
Ano de publicação: 	2009	
Fonte/Imprenta: 	In: ISHS/PROMUSA BANANA SYMPOSIUM, 2009, Guangzhou, China. Global perspectives on asian challenges: programme and abstracts. Guangzhou: ISHS: Promusa, 2009. p. 10.	
Idioma: 	Inglês	
Conteúdo: 	The advent of high-throughput sequencing technology has generated abundant information on DNA sequences for the genomes of many plant species. Expressed Sequence Tag (EST), a unique DNA sequence derived from a cDNA library and therefore representing a gene which has been transcribed in a specific tissue or at some stage of development , is one type of DNA sequence made highly available lately for many important crop species. Molecular markers are used for bridging DNA sequence information with particular phenotypes and are useful tools for genotyping germplasm collections and also for tagging genes governing desirable agronomic traits. In this sense, there is always a strong demand for better marker techniques to better utilise the existing sequence information. A transcriptome database from banana (Musa spp.), DATAMusa, containing 42,724 ESTs from 11 different cDNA libraries and encompassing approximately  24 Mb of DNA sequence, was used in this study for the design of primers to PCR amplify txo types of EST-derived molecular markers, Variable Nucleotide Tandem Repeat (VNTR) and Target Region Amplification Polymorphism (TRAP). These primers were then validated against a panel of 14 Musa diploid genotypes and produced 33 (VNTR) and 119 (TRAP) alleles. Used separately or together, both types of markers were able to discriminate Musa genotypes from different genome background (A or B genomes). The TRAP alleles identified derived from only one unigene, while the VNTR derived from 12 unigenes. Based on the results from this study, it can be said that EST-derived markers are an important source of polymorphism to be used in genetic diversity and gene discovery studies in banana.	
Thesagro: 	Banana; Marcador Molecular.	
Thesaurus Nal: 	Musa.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)