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| Registros recuperados : 82 | |
| 41. |  | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; OLIVEIRA, J. C.; GARCIA, J. M. Effects of demecolcine on microtubule composition and chemically assisted enucleation of bovine oocytes. Reproduction, Fertility and Development, v. 20, n. 1, p. 107-108, 2008.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 42. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; COLLADO, M. D.; LIMA, M. R.; NICIURA, S. C. M.; FIGUEIRO, M. R.; VANTINI, R.; GARCIA, J. M. Efeitos do uso de moduladores de AMPC durante a pré-maturação in vitro de oócitos bovinos sobre as junções comunicantes e o potencial de senvolvimento. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. 1| Biblioteca(s): Embrapa Gado de Leite. |
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| 43. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; OLIVEIRA, C. S.; MONTEIRO, F. M.; LIMA, M. R.; NICIURA, S. C. M.; FERREIRA, C. R.; GARCIA, J. M. Effects of embryonic fluid and serum replacer as protein sources for in vitro maturation of bovine oocytes. In: INTERNATIONAL SYMPOSIUM ANIMAL BIOLOGY OF REPRODUCTIVE, 3., 2010, Águas de São Pedro: CBRA: USP/FMVZ, 2010| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 44. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; NICIURA, S. C. M.; FERREIRA, C. R.; OLIVEIRA, C. S.; GARCIA, J. M. The effects of ovalbumin as a protein source during the in vitro production of bovine embryos. Revista Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, oct. 2011.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 45. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; COLLADO, M. del; LIMA, M. R.; MEO, S. C.; FIGUEIRO, M. R.; VANTINI, R.; GARCIA, J. M. Effects of cAMP modulators during in vitro prematuration of bovine oocytes on gap junctional communication and embryo development potential. Animal Reproduction, v. 13, n. 3, p. 602, Jul./Sep. 2016.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 46. | | CRUZ, M. H. C.; SARAIVA, N. Z.; CRUZ, J. F. da; OLIVEIRA, C. S.; COLLADO, M. del; FERNANDES, H.; CASTRO, F. C. de; GARCIA, J. M. Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro. Revista Brasileira de Zootecnia, v. 43, n. 3, p. 120-126, 2014.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 47. | | DEL COLLADO, M.; SARAIVA, N. Z.; LOPES, F. L.; CRUZ, M. H.; GASPAR, R. C.; OLIVEIRA, C. S.; PERECIN, F.; GARCIA, J. M. Efeitos da redução ou substituição do soro fetal bovino por outros compostos na maturação in vitro de oócitos bovinos. Pesquisa Veterinária Brasileira v. 34, n. 7, p. 689-694, jul. 2014.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 48. | | LIMA, V. F. M. H. de; GARCIA, J. M.; MOREIRA FILHO, C. A.; COTINO C.; BEM, A. R. de; KIRSZEMBAUM, M. Identificacao do sexo de embrioes bovinos por imunofluorescencia indireta. In: REUNIAO DA SOCIEDADE BRASILEIRA DE TRANSFERENCIA DE EMBRIOES, 7., 1992, Jaboticabal, SP. Anais. [S.l.: s.n.], 1992. p.109. p.109| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 49. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; CRUZ, M. H. C.; MAZETI, B.; OLIVEIRA, L. Z.; LOPES, F. L.; GARCIA, J. M. HDAC inhibition decreases XIST expression on female IVP bovine blastocysts. Reproduction, v. 145, n. 1, p. 9-17, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 50. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; SOUZA, M. M.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. Bovine cytoplasts prepared by demecolcine-induced enucleation of activated oocytes. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, 2010. p. 197| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 51. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; SOUZA, M. M.; LIMA, M. R.; NICIURA, S. C. M.; GARCIA, J. M. Métodos alternativos de enucleação oocitária utilizados na transferência nuclear em animais. Revista Brasileira de Reprodução Animal, v. 34, n. 4, p. 197-205, out./dez. 2010.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 52. | | COLLADO, M. del; SARAIVA, N. Z.; LOPES, F. L.; GASPAR, R. C.; PADILHA, L. C.; COSTA, R. R.; ROSSI, G. F.; VANTINI, R.; GARCIA, J. M. Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos. Reproduction, Fertility and Development, v. 28, n. 11, p. 1721-1732, 2016.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 54. | | FERREIRA, C. R.; BURGSTALLER, J. P.; PERECIN, F.; GARCIA, J. M.; CHIARATTI, M. R.; MÉO, S. C.; MULLER, M.; SMITH, L. C.; MEIRELLES, F. V.; STEINBORN, R. Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line. Biology of Reproduction, v. 82, n. 03, p. 563-571, mar. 2010.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 55. | | OLIVEIRA, S. A. S. de; ABREU, E. F. M.; ARAÚJO, T. S.; OLIVEIRA, E. J. de; ANDRADE, E. C. de; GARCIA, J. M. P.; ÁLVAREZ, E. First Report of a 16SrIII-L Phytoplasma Associated with Frogskin Disease in Cassava (Manihot esculenta Crantz) in Brazil. Plant Disease, v.98, n.1, p.153, Jan., 2014. Abstract.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 56. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. Expressão dos genes xist, g6pd e hspa1 em blastocistos bovinos reconstituídos por tn a partir de oócitos receptores produzidos por enucleação assistida quimicamente. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco. O impacto das biotecnologias reprodutivas na saúde e produção animal - anais. Cumbuco: SBTE, 2011. p. 437| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 57. | | NICIURA, S. C. M.; FERREIRA, C. R.; PERECIN, F.; YAMAZAKI, W.; LEAL, C. L. M.; GARCIA, J. M.; MEIRELLES, F. V. Desenvolvimento embrionário, visualização de pronúcleos e transferência pronuclear após centrifugação de zigotos bovinos em meio com citocalasina. São Carlos, SP: Embrapa Pecuária Sudeste, 2007 34 p. (Embrapa Pecuária Sudeste, Boletim de pesquisa e desenvolvimento,11). ISSN 1981-2078| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 58. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes. Genetics and Molecular Research, v. 8, n. 1, p. 76-85, 2009.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 59. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; BIASE, F. H.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas. Reproduction, Fertility and Development, v. 20, n. 1, p. 173, 2008.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 60. | | MEO, S. C.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; TETZNER, T. A. D.; YAMAZAKI, W.; LEA, C. L. V.; MEIRELLES, F. V.; GARCIA, J. M. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle. Animal Reproduction Science, v. 116, n. 3-4, p. 381-385, dec. 2009.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 82 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
06/12/2011 |
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Data da última atualização: |
28/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; NICIURA, S. C. M.; FERREIRA, C. R.; OLIVEIRA, C. S.; GARCIA, J. M. |
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Afiliação: |
TATIANE ALMEIDA DRUMMOND TETZNER, UNESP/JABOTICABAL; NAIARA ZOCCAL SARAIVA, UNESP/JABOTICABAL; FELIPE PERECIN, FZEA-USP/PIRASSUNUNGA; SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTNA RAMIRES FERREIRA, UNICAMP/CAMPINAS; CLARA SLADE OLIVEIRA, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
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Título: |
The effects of ovalbumin as a protein source during the in vitro production of bovine embryos. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, oct. 2011. |
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DOI: |
https://doi.org/10.1590/S1516-35982011001000010 |
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Idioma: |
Inglês |
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Conteúdo: |
Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production. MenosEmbryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in... Mostrar Tudo |
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Palavras-Chave: |
Bovine embryo; Protein source. |
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Thesaurus NAL: |
in vitro culture; ovalbumin. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/49368/1/PROCI2011.00188.pdf
|
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Marc: |
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024 7 $ahttps://doi.org/10.1590/S1516-35982011001000010$2DOI
100 1 $aTETZNER, T. A. D.
245 $aThe effects of ovalbumin as a protein source during the in vitro production of bovine embryos.$h[electronic resource]
260 $c2011
520 $aEmbryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production.
650 $ain vitro culture
650 $aovalbumin
653 $aBovine embryo
653 $aProtein source
700 1 $aSARAIVA, N. Z.
700 1 $aPERECIN, F.
700 1 $aNICIURA, S. C. M.
700 1 $aFERREIRA, C. R.
700 1 $aOLIVEIRA, C. S.
700 1 $aGARCIA, J. M.
773 $tRevista Brasileira de Zootecnia$gv. 40, n. 10, p. 2135-2141, oct. 2011.
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Embrapa Pecuária Sudeste (CPPSE) |
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