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| Registros recuperados : 82 | |
| 21. |  | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; VANTINI, R.; GARCIA, J. M. Efeitos da demecolcina sobre a cinética da maturação nuclear e a migração dos grânulos corticais em oócitos bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1276, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007. p. 1276.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 22. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; DEL COLLADO, M.; LIMA, M. R.; CRUZ, M. H.; VANTINI, R.; GARCIA, J. M. Efeitos da pré-maturação in vitro de oócitos bovinos com moduladores de AMPc sobre a progressão nuclear. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 26., 2012, Foz do Iguaçu. Anais. Belo Horizonte: CBRA, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 23. | | SARAIVA, N. Z.; FIGUEIRO, M. R.; MARQUES, J. R. F.; LIMA, M. R. de; GARCIA, J. M. Effect of in vitro co-culture of buffalo embryos with bovine cumulus cells on the potential for early embryonic development. Animal Reproduction, v. 14, n. 3, p. 858, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 28. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; OLIVEIRA, L. Z.; SERAPIÃO, R. V.; LIMA, M. R. DE; GARCIA, J. M. Global H3K9AC and H3K27ME3 expression in blastomeres from 8-to 16-cell stage bovine embryos. Reproduction, Fertility and Development , v. 26, n. 1, p. 148-149, 2014. Suplemento. Proceedings da Annual Conference of the International Embryo Transfer Societty, 2014, Reno.| Biblioteca(s): Embrapa Gado de Leite. |
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| 29. | | MÉO, S. C.; YAMAZAKI, W.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; LEAL, C. L. V.; GARCIA, J. M. Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments. Zygote, v. 15, p. 295-306, nov. 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 30. | | OLIVEIRA, C. S.; JEDRUSIK, A.; SARAIVA, N. Z.; CAMARGO, L. S. de A.; GARCIA, J. M.; ZERNICKA-GOETZ, M. PAR6C polarity protein expression pattern in male and female bovine embryos. Animal Reproduction, v. 11, n. 3, p. 455, 2014. Edição dos proceedings do 28º Annual Meeting of the Brazilian Embryo Technology Society, 2014, Natal.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 31. | | LANDIM JR, L. P.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; MIGLINO, M. A.; GARCIA, J. M. Análise da expressao gênica do VEGF (Vascular Endothelial Growth Factor) em placentas bovinas de animais clonados, produzidos in vitro e in vivo, pelo método de RT-PCR semiquantitativo. ARS VETERINARIA, v. 22, n. 3, p. 217-222, 2006.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 32. | | OLIVEIRA, C. S.; SERAPIÃO, R. V.; SARAIVA, N. Z.; CAMARGO, A. J. dos R.; FERREIRA, M. B. D.; FREITAS, C. de; GARCIA, J. M.; VERNEQUE, R. da S. Análise quantitativa de lipídeos em oócitos de vacas leiteiras das raças Gir Leiteiro, Holandesa, e mestiças F1. In: CONGRESSO INTERNACIONAL DO LEITE, 12.; WORKSHOP DE POLÍTICAS PÚBLICAS, 12.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 13., 2013, Porto Velho. Anais... Brasília, DF: Embrapa, 2013. 3 p. 1 CD-ROM.| Biblioteca(s): Embrapa Gado de Leite. |
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| 33. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; SOUZA, M. M.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. Bovine cytoplasts prepared by demecolcine-induced enucleation of activated oocytes. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, 2010. p. 197| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 34. | | ROSA, P. M. S.; MONTEIRO, C. A. S.; LEAL, G. R.; CAMARGO, A. J. R.; SERAPIÃO, R. V.; LIMA, M. R.; GARCIA, J. M.; OLIVEIRA, C. S. Association of two epigenetic modulators on the initial development of female bovine embryos. Animal Reproduction, v. 15, n. 3, p. 484, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Biblioteca(s): Embrapa Gado de Leite. |
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| 35. | | NICIURA, S. C. M.; FERREIRA, C. R.; PERECIN, F.; YAMAZAKI, W.; LEAL, C. L. M.; GARCIA, J. M.; MEIRELLES, F. V. Desenvolvimento embrionário, visualização de pronúcleos e transferência pronuclear após centrifugação de zigotos bovinos em meio com citocalasina. São Carlos, SP: Embrapa Pecuária Sudeste, 2007 34 p. (Embrapa Pecuária Sudeste, Boletim de pesquisa e desenvolvimento,11). ISSN 1981-2078| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 36. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; LIMA, M. R. de; OLIVEIRA, L. Z.; SERAPIÃO, R. V.; GARCIA, J. M.; BORGES, C. A. V.; CAMARGO, L. S. de A. Cell death is involved in sexual dimorphism during preimplantation development. Mechanisms of Development v. 139, p. 42-50, 2016.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 37. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; MELO, D. S. de; NICIURA, S. C. M.; GARCIA, J. M. Chemically assisted enucleation results in higher G6PD expression in early bovine female embryos obtained by somatic cell nuclear transfer. Cellular Reprogramming, v. 14, n. 5, p. 1-11, 2012.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 38. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. Zygote, v. 23, n. 6, p. 852-862, 2015.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
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| 40. | | FERREIRA, C. R.; BURGSTALLER, J. P.; PERECIN, F.; GARCIA, J. M.; CHIARATTI, M. R.; MÉO, S. C.; MULLER, M.; SMITH, L. C.; MEIRELLES, F. V.; STEINBORN, R. Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line. Biology of Reproduction, v. 82, n. 03, p. 563-571, mar. 2010.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 82 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
06/12/2011 |
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Data da última atualização: |
28/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; NICIURA, S. C. M.; FERREIRA, C. R.; OLIVEIRA, C. S.; GARCIA, J. M. |
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Afiliação: |
TATIANE ALMEIDA DRUMMOND TETZNER, UNESP/JABOTICABAL; NAIARA ZOCCAL SARAIVA, UNESP/JABOTICABAL; FELIPE PERECIN, FZEA-USP/PIRASSUNUNGA; SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTNA RAMIRES FERREIRA, UNICAMP/CAMPINAS; CLARA SLADE OLIVEIRA, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
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Título: |
The effects of ovalbumin as a protein source during the in vitro production of bovine embryos. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, oct. 2011. |
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DOI: |
https://doi.org/10.1590/S1516-35982011001000010 |
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Idioma: |
Inglês |
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Conteúdo: |
Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production. MenosEmbryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in... Mostrar Tudo |
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Palavras-Chave: |
Bovine embryo; Protein source. |
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Thesaurus NAL: |
in vitro culture; ovalbumin. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/49368/1/PROCI2011.00188.pdf
|
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Marc: |
LEADER 02467naa a2200253 a 4500
001 1908631
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008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1516-35982011001000010$2DOI
100 1 $aTETZNER, T. A. D.
245 $aThe effects of ovalbumin as a protein source during the in vitro production of bovine embryos.$h[electronic resource]
260 $c2011
520 $aEmbryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production.
650 $ain vitro culture
650 $aovalbumin
653 $aBovine embryo
653 $aProtein source
700 1 $aSARAIVA, N. Z.
700 1 $aPERECIN, F.
700 1 $aNICIURA, S. C. M.
700 1 $aFERREIRA, C. R.
700 1 $aOLIVEIRA, C. S.
700 1 $aGARCIA, J. M.
773 $tRevista Brasileira de Zootecnia$gv. 40, n. 10, p. 2135-2141, oct. 2011.
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Embrapa Pecuária Sudeste (CPPSE) |
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