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| Registros recuperados : 81 | |
| 61. |  | LIMA, V. F. M. H. de; GARCIA, J. M.; MOREIRA FILHO, C. A.; COTINO C.; BEM, A. R. de; KIRSZEMBAUM, M. Identificacao do sexo de embrioes bovinos por imunofluorescencia indireta. In: REUNIAO DA SOCIEDADE BRASILEIRA DE TRANSFERENCIA DE EMBRIOES, 7., 1992, Jaboticabal, SP. Anais. [S.l.: s.n.], 1992. p.109. p.109| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 62. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes. Genetics and Molecular Research, v. 8, n. 1, p. 76-85, 2009.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 63. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; BIASE, F. H.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas. Reproduction, Fertility and Development, v. 20, n. 1, p. 173, 2008.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 64. | | COLLADO, M. del; SARAIVA, N. Z.; LOPES, F. L.; GASPAR, R. C.; PADILHA, L. C.; COSTA, R. R.; ROSSI, G. F.; VANTINI, R.; GARCIA, J. M. Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos. Reproduction, Fertility and Development, v. 28, n. 11, p. 1721-1732, 2016.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 65. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; CRUZ, M. H. C.; MAZETI, B.; OLIVEIRA, L. Z.; LOPES, F. L.; GARCIA, J. M. HDAC inhibition decreases XIST expression on female IVP bovine blastocysts. Reproduction, v. 145, n. 1, p. 9-17, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 66. | | MONTEIRO, F. M.; MERCADANTE, M. E. Z.; BONILHA, S. F. M.; OLIVEIRA, L. Z.; SARAIVA, N. Z.; OLIVEIRA, C. S.; GARCIA, J. M. Ultrasonographic evaluation of reproductive tract measures and fat thickness traits in pre-pubertal nellore heifers. Boletim de Indústria Animal, v. 69, n. 2, p. 129-135, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 67. | | FEUCHARD, V. L. da S.; MARQUES, S. C. de S.; SERAPIÃO, R. V.; SARAIVA, N. Z.; LIMA, M. R. de; GARCIA, J. M.; OLIVEIRA, C. S. Use of lipolysis supplement during IVM reduces lipid content of bovine oocytes but do not affect blastocyst cryosurvival. In: Annual Meeting of the Braziliam Embrio Technology Society, 33rd. Resumo em Anais. Ilha de Comandatuba, Bahia. SBTE, 2019. p 644.| Biblioteca(s): Embrapa Gado de Leite. |
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| 68. | | PERECIN, F.; YAMAZAKI, W.; FERREIRA, C. R.; MÉO, S. C.; BIASE, F. H.; MERIGHE, G. K. F.; SARAIVA, N. Z.; TETZNER, T. A. D.; MEIRELLES, F. V.; GARCIA, J. M. Expressão de DNA metiltransferases em blastocistos bovinos produzidos in vivo e in vitro. Acta Scientiae Veterinariae, v. 35, supl. 3, p. 1181, 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 69. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; MELO, D. S.; MONTEIRO, F. M.; LIMA, M. R.; LEAL, C. L. V.; NICIURA, S. C. M.; GARCIA, J. M. Evaluation of MPF and MAPK activities in recipient bovine oocytes produced by chemical enucleation techniques in nuclear transfer. In: INTERNATIONAL SYMPOSIUM ANIMAL BIOLOGY OF REPRODUCTIVE, 3., 2010, Águas de São Pedro: CBRA: USP/FMVZ, 2010| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 70. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; FERRERIA, C. R.; NICIURA, S. C. M.; OLIVEIRA, C. MELO, D. S.; MELO, D. S.; MONTEIRO, F. M.; VANTINI, R.; GARCIA, J. M. Avaliação da qualidade em embriões PIV com a substituição do soro fetal bovino e da albumina sérica bovina pela ovalbumina. Acta Scientiae Veterinariae, v. 36, (Supl. 2), 2008.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 71. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; FERREIRA, C. R.; MÉO, S. C.; OLIVEIRA, C. S.; MELO, D. S.; MONTEIRO, F. M.; VANTINI, R.; GARCIA, J. M. Avaliação da influência da ovalbumina (OVA) como suplemento protéico durante a etapa de fecundação in vitro de oócitos bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1181, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 72. | | MIGLINO, M. A.; PEREIRA, F. T. V.; VISINTIN, J. A.; GARCIA, J. M.; MEIRELLES, F. V.; RUMPF, R.; AMBRÓSIO, C. E.; PAPA, P. C.; SANTOS, T. C.; CARVALHO, A. F.; LEISER, R.; CARTER, A. M. Placentation in cloned cattle: structure and microvascular architecture. Theriogenology, v. 68, p. 604-617, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 73. | | NICIURA, S. C. M.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; TETZNER, T. A. D.; BORGES, J. C.; YAMAZAKI, W.; LEAL, C. L. V.; MEIRELLES, F. V.; GARCIA, J. M. Troca de carioplasto entre zigotos ativados com estrôncio ou 6-DMAP. Acta Scientiae Veterinariae, Porto Alegre, v. 35, Supl. 3, 2007. p. s1269. Edição de anais da 21a. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 74. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; LIMA, M. R. de; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. Zygote, v. 23, n. 6, p. 852-862, Dec. 2015.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 75. | | CHIARATTI, M. R.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; SANGALLI, J. R.; MESQUITA, L. G.; BALIEIRO, J. C. de C.; SMITH, L. C.; GARCIA, J. M.; MEIRELLES, F. V. Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. Reproductive BioMedicine Online v. 22, n. 22, p. 172-183, 2011.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 76. | | PERECIN, F.; YAMAZAKI, W.; FERREIRA, C. R.; MÉO, S. C.; BIASE, F. H.; MERIGHE, G. K. F.; SARAIVA, N. Z.; TETZNER, T. A. D.; MEIRELLES, F. V.; GARCIA, J. M. Efeito do protocolo de sincronização celular na produção de clones bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1181, 2007. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007. p. 1267| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 77. | | MONTEIRO, C. A. S.; SARAIVA, H. F. R. A.; CARMARGO, A. J. R.; ROSA, P. S.; RODRIGUES, A. L. R.; SERAPIÃO, R. V.; NOGUEIRA, L. A. G.; CAMARGO, L. S. de A.; GARCIA, J. M.; OLIVEIRA, C. S. Effect of camp modulalators during oocyte in vitro maturation on gap junctional activity of vitrifief bovine oocytes In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2016, Louisville. Reproduction, fertility and development: proceedings. Louisville: [s.n.], 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 78. | | MONTEIRO, C. A. S.; LEAL, G. R.; SARAIVA, H. F. R. A.; CAMARGO, A. J. R.; RODRIGUES, A. L. R.; SERAPIÃO, R. V.; NOGUEIRA, L. A. G.; CAMARGO, L. S. de A.; GARCIA, J. M.; OLIVEIRA, C. S. Effect of cAMP modulators during oocyte in vitro maturation on nuclear maturation and cytoskeleton integrity of vitrified bovine oocytes. In: ANNUAM MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 29., 2015, Gramado. Abstracts... Gramado: SBTE, 2015.| Biblioteca(s): Embrapa Gado de Leite. |
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| 79. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; BARROS, F. F. P. C.; LOPES, F. L.; CAPANHA, B. C. S.; NOGUEIRA, M. F. G.; SERAPIÃO, R. V.; MONTEIRO, F. M.; GARCIA, J. M. Effects of in vitro culture on epigenetic control of bivalent domains (H3K4ME3 e H3K27ME3) and expression of neuronal genes in cortical neurons. In: CONGRESSO BRASILEIRO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Águas de Lindóia: Sociedade Brasileira de Genética, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 80. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; FERREIRA, C. R.; MÉO, S. C.; OLIVEIRA, C. S.; MELO, D. S.; MONTEIRO, F. M.; VANTINI, R.; GARCIA, J. M. Efeitos da substituição do soro fetal bovino (SFB) e da albumina sérica bovina (BSA) pela ovalbumina (OVA) na produção in vitro de embriões bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1181, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 81 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
14/02/2011 |
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Data da última atualização: |
19/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CHIARATTI, M. R.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; SANGALLI, J. R.; MESQUITA, L. G.; BALIEIRO, J. C. de C.; SMITH, L. C.; GARCIA, J. M.; MEIRELLES, F. V. |
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Afiliação: |
MARCOS ROBERTO CHIARATTI, USP/PIRASSUNGA; CHRISTINA RAMIRES FERREIRA, USP/PIRASSUNGA; FELIPE PERECIN, USP/PIRASSUNUNGA; SIMONE CRISTINA MEO NICIURA, CPPSE; JULIANO RODRIGUES SANGALLI, USP/PIRASSUNUGA; LÍGIA GARCIA MESQUITA, USP/PIRASSUNGA; JÚLIO CÉSAR DE CARVALHO BALIEIRO, USP/PIRASSUNUNGA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL/SAINT-HYACINTHE; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL; FLÁVIO VIEIRA MEIRELLES, USP/PIRASSUNUNGA. |
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Título: |
Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Reproductive BioMedicine Online v. 22, n. 22, p. 172-183, 2011. |
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DOI: |
https://doi.org/10.1016/j.rbmo.2010.10.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Ooplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes. MenosOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to ... Mostrar Tudo |
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Palavras-Chave: |
Embryo; Ethidium bromide; Oocyte; Ooplasm; Transfer. |
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Thesaurus NAL: |
mitochondrial DNA. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/876985/1/OoplastMediatedDevelopmental.pdf
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Marc: |
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100 1 $aCHIARATTI, M. R.
245 $aOoplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide.$h[electronic resource]
260 $c2011
520 $aOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes.
650 $amitochondrial DNA
653 $aEmbryo
653 $aEthidium bromide
653 $aOocyte
653 $aOoplasm
653 $aTransfer
700 1 $aFERREIRA, C. R.
700 1 $aPERECIN, F.
700 1 $aNICIURA, S. C. M.
700 1 $aSANGALLI, J. R.
700 1 $aMESQUITA, L. G.
700 1 $aBALIEIRO, J. C. de C.
700 1 $aSMITH, L. C.
700 1 $aGARCIA, J. M.
700 1 $aMEIRELLES, F. V.
773 $tReproductive BioMedicine Online$gv. 22, n. 22, p. 172-183, 2011.
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