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| Registros recuperados : 82 | |
| 41. |  | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; OLIVEIRA, J. C.; GARCIA, J. M. Effects of demecolcine on microtubule composition and chemically assisted enucleation of bovine oocytes. Reproduction, Fertility and Development, v. 20, n. 1, p. 107-108, 2008.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 42. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; COLLADO, M. D.; LIMA, M. R.; NICIURA, S. C. M.; FIGUEIRO, M. R.; VANTINI, R.; GARCIA, J. M. Efeitos do uso de moduladores de AMPC durante a pré-maturação in vitro de oócitos bovinos sobre as junções comunicantes e o potencial de senvolvimento. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. 1| Biblioteca(s): Embrapa Gado de Leite. |
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| 43. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; OLIVEIRA, C. S.; MONTEIRO, F. M.; LIMA, M. R.; NICIURA, S. C. M.; FERREIRA, C. R.; GARCIA, J. M. Effects of embryonic fluid and serum replacer as protein sources for in vitro maturation of bovine oocytes. In: INTERNATIONAL SYMPOSIUM ANIMAL BIOLOGY OF REPRODUCTIVE, 3., 2010, Águas de São Pedro: CBRA: USP/FMVZ, 2010| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 44. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; NICIURA, S. C. M.; FERREIRA, C. R.; OLIVEIRA, C. S.; GARCIA, J. M. The effects of ovalbumin as a protein source during the in vitro production of bovine embryos. Revista Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, oct. 2011.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 45. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; COLLADO, M. del; LIMA, M. R.; MEO, S. C.; FIGUEIRO, M. R.; VANTINI, R.; GARCIA, J. M. Effects of cAMP modulators during in vitro prematuration of bovine oocytes on gap junctional communication and embryo development potential. Animal Reproduction, v. 13, n. 3, p. 602, Jul./Sep. 2016.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 46. | | CRUZ, M. H. C.; SARAIVA, N. Z.; CRUZ, J. F. da; OLIVEIRA, C. S.; COLLADO, M. del; FERNANDES, H.; CASTRO, F. C. de; GARCIA, J. M. Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro. Revista Brasileira de Zootecnia, v. 43, n. 3, p. 120-126, 2014.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 47. | | DEL COLLADO, M.; SARAIVA, N. Z.; LOPES, F. L.; CRUZ, M. H.; GASPAR, R. C.; OLIVEIRA, C. S.; PERECIN, F.; GARCIA, J. M. Efeitos da redução ou substituição do soro fetal bovino por outros compostos na maturação in vitro de oócitos bovinos. Pesquisa Veterinária Brasileira v. 34, n. 7, p. 689-694, jul. 2014.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 48. | | LIMA, V. F. M. H. de; GARCIA, J. M.; MOREIRA FILHO, C. A.; COTINO C.; BEM, A. R. de; KIRSZEMBAUM, M. Identificacao do sexo de embrioes bovinos por imunofluorescencia indireta. In: REUNIAO DA SOCIEDADE BRASILEIRA DE TRANSFERENCIA DE EMBRIOES, 7., 1992, Jaboticabal, SP. Anais. [S.l.: s.n.], 1992. p.109. p.109| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 49. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; CRUZ, M. H. C.; MAZETI, B.; OLIVEIRA, L. Z.; LOPES, F. L.; GARCIA, J. M. HDAC inhibition decreases XIST expression on female IVP bovine blastocysts. Reproduction, v. 145, n. 1, p. 9-17, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 50. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; SOUZA, M. M.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. Bovine cytoplasts prepared by demecolcine-induced enucleation of activated oocytes. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, 2010. p. 197| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 51. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; SOUZA, M. M.; LIMA, M. R.; NICIURA, S. C. M.; GARCIA, J. M. Métodos alternativos de enucleação oocitária utilizados na transferência nuclear em animais. Revista Brasileira de Reprodução Animal, v. 34, n. 4, p. 197-205, out./dez. 2010.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 52. | | COLLADO, M. del; SARAIVA, N. Z.; LOPES, F. L.; GASPAR, R. C.; PADILHA, L. C.; COSTA, R. R.; ROSSI, G. F.; VANTINI, R.; GARCIA, J. M. Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos. Reproduction, Fertility and Development, v. 28, n. 11, p. 1721-1732, 2016.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 54. | | FERREIRA, C. R.; BURGSTALLER, J. P.; PERECIN, F.; GARCIA, J. M.; CHIARATTI, M. R.; MÉO, S. C.; MULLER, M.; SMITH, L. C.; MEIRELLES, F. V.; STEINBORN, R. Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line. Biology of Reproduction, v. 82, n. 03, p. 563-571, mar. 2010.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 55. | | OLIVEIRA, S. A. S. de; ABREU, E. F. M.; ARAÚJO, T. S.; OLIVEIRA, E. J. de; ANDRADE, E. C. de; GARCIA, J. M. P.; ÁLVAREZ, E. First Report of a 16SrIII-L Phytoplasma Associated with Frogskin Disease in Cassava (Manihot esculenta Crantz) in Brazil. Plant Disease, v.98, n.1, p.153, Jan., 2014. Abstract.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 56. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. Expressão dos genes xist, g6pd e hspa1 em blastocistos bovinos reconstituídos por tn a partir de oócitos receptores produzidos por enucleação assistida quimicamente. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco. O impacto das biotecnologias reprodutivas na saúde e produção animal - anais. Cumbuco: SBTE, 2011. p. 437| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 57. | | NICIURA, S. C. M.; FERREIRA, C. R.; PERECIN, F.; YAMAZAKI, W.; LEAL, C. L. M.; GARCIA, J. M.; MEIRELLES, F. V. Desenvolvimento embrionário, visualização de pronúcleos e transferência pronuclear após centrifugação de zigotos bovinos em meio com citocalasina. São Carlos, SP: Embrapa Pecuária Sudeste, 2007 34 p. (Embrapa Pecuária Sudeste, Boletim de pesquisa e desenvolvimento,11). ISSN 1981-2078| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 58. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes. Genetics and Molecular Research, v. 8, n. 1, p. 76-85, 2009.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 59. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; BIASE, F. H.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas. Reproduction, Fertility and Development, v. 20, n. 1, p. 173, 2008.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 60. | | MEO, S. C.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; TETZNER, T. A. D.; YAMAZAKI, W.; LEA, C. L. V.; MEIRELLES, F. V.; GARCIA, J. M. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle. Animal Reproduction Science, v. 116, n. 3-4, p. 381-385, dec. 2009.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 82 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
24/03/2008 |
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Data da última atualização: |
22/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; OLIVEIRA, J. C.; GARCIA, J. M. |
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Afiliação: |
Naiara Z. Saraiva, UNESP/Jaboticabal; Felipe Perecin USP/Pirassununga Simone Crisitina Méo CPPSE; Christina Ramires Ferreira, Unicamp; Tatiane Almeida D. Tetzner, UNESP/Jaboticabal; J. M. Oliveira, UNESP/Jaboticabal; Joaquim Mansano Garcia, UNESP/Jaboticabal. |
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Título: |
Effects of demecolcine on microtubule composition and chemically assisted enucleation of bovine oocytes. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 20, n. 1, p. 107-108, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
The developmental competence of enucleated oocytes is a key factor that determines the overall success of animal cloning. Enucleation is an invasive procedure in traditional nuclear transfer (NT). The objective of this work was to evaluate the effects of demecolcine, a microtubule-depolymerizing agent, on metaphase II (MII) bovine oocytes and to verify the capacity of embryonic development after NT using chemically assisted enucleation. In the first experiment, oocytes after 21 h of IVM were exposed for 2 h to several concentrations of demecolcine: 0 (control), 0.025, 0.05, 0.2, and 0.4 µg mL?1, and evaluated in relation to membrane protrusion formation. After the best concentration of demecolcine was determined, the nuclear and microtubular dynamics of the treated oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 5, 537?545) in a second experiment. The results were analyzed by Duncan and Tukey tests in Experiments I and II, respectively, and a level of 5% significance was used. In Experiment III, embryonic development following NT was determined using adult donor cells, derived from a skin biopsy. After 19 h of IVM, oocytes were exposed to demecolcine (0.05 µg mL?1) for 2 h, and enucleation was performed in oocytes that presented membrane protrusions. Samples of cytoplasts were stained with Hoechst 33342 for 10 min (10 µg mL?1) for evaluation of enucleation effectiveness. The remaining oocytes were reconstituted by NT, fused (two pulses of 2.0 kV cm?1 for 20 µs each, in 0.28 m mannitol solution), chemically activated (5 mm ionomycin for 5 min and 2 mm 6-DMAP for 4 h), and cultured in SOF medium with 2.5% FCS and 0.5% BSA. The 0.05 µg mL?1 concentration resulted in greater protrusion rates (55.1%; 211/388) in comparison to 0, 0.025, 0.2, and 0.4 µg mL?1 concentrations (0, 42.6, 45.1, and 39.3%, respectively). In Experiment II, at the beginning of treatment, the majority of the oocytes were in MII (40.7%) or anaphase I/telophase I (AI/TI; 22.4%). Effects of demecolcine occurred within only 0.5 h of treatment, with a significant increase in oocytes with complete depletion of microtubules (21.8%; initial average: 1.9%) and a reduction in the proportion at MII (13.5%) and AI/TI (8.2%). New polymerization of microtubules was observed when treated oocytes were then cultured in drug-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). In Experiment III, we verified the effectiveness of the chemically assisted enucleation technique (90.6%; 77/85). We evaluated 515 oocytes, of which 219 (42.5%) had protrusions and were enucleated. After losses in the NT procedure and fusion, 58 reconstructed 1-cells were cultured resulting in cleavage and blastocyst rates of 84.5% (49/58) and 27.6% (16/58), respectively, with great variation in blastocyst production between the three replicates (12.5% to 47%). In conclusion, demecolcine can be used at lower concentrations than those used routinely, and the chemically assisted enucleation method has proven highly efficient and does not appear to inhibit embryonic development in the bovine MenosThe developmental competence of enucleated oocytes is a key factor that determines the overall success of animal cloning. Enucleation is an invasive procedure in traditional nuclear transfer (NT). The objective of this work was to evaluate the effects of demecolcine, a microtubule-depolymerizing agent, on metaphase II (MII) bovine oocytes and to verify the capacity of embryonic development after NT using chemically assisted enucleation. In the first experiment, oocytes after 21 h of IVM were exposed for 2 h to several concentrations of demecolcine: 0 (control), 0.025, 0.05, 0.2, and 0.4 µg mL?1, and evaluated in relation to membrane protrusion formation. After the best concentration of demecolcine was determined, the nuclear and microtubular dynamics of the treated oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 5, 537?545) in a second experiment. The results were analyzed by Duncan and Tukey tests in Experiments I and II, respectively, and a level of 5% significance was used. In Experiment III, embryonic development following NT was determined using adult donor cells, derived from a skin biopsy. After 19 h of IVM, oocytes were exposed to demecolcine (0.05 µg mL?1) for 2 h, and enucleation was performed in oocytes that presented membrane protrusions. Samples of cytoplasts were stained with Hoechst 33342 for 10 min (10 µg mL?1) for evaluation of enucleation effectiveness. The remaining oocytes were reconstituted ... Mostrar Tudo |
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Thesagro: |
BOVINO. |
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Thesaurus NAL: |
oocytes. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42090/1/PROCI-2008.00005.pdf
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Marc: |
LEADER 03796nam a2200205 a 4500
001 1048309
005 2023-06-22
008 2008 bl uuuu u00u1 u #d
100 1 $aSARAIVA, N. Z.
245 $aEffects of demecolcine on microtubule composition and chemically assisted enucleation of bovine oocytes.$h[electronic resource]
260 $aReproduction, Fertility and Development, v. 20, n. 1, p. 107-108, 2008.$c2008
520 $aThe developmental competence of enucleated oocytes is a key factor that determines the overall success of animal cloning. Enucleation is an invasive procedure in traditional nuclear transfer (NT). The objective of this work was to evaluate the effects of demecolcine, a microtubule-depolymerizing agent, on metaphase II (MII) bovine oocytes and to verify the capacity of embryonic development after NT using chemically assisted enucleation. In the first experiment, oocytes after 21 h of IVM were exposed for 2 h to several concentrations of demecolcine: 0 (control), 0.025, 0.05, 0.2, and 0.4 µg mL?1, and evaluated in relation to membrane protrusion formation. After the best concentration of demecolcine was determined, the nuclear and microtubular dynamics of the treated oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 5, 537?545) in a second experiment. The results were analyzed by Duncan and Tukey tests in Experiments I and II, respectively, and a level of 5% significance was used. In Experiment III, embryonic development following NT was determined using adult donor cells, derived from a skin biopsy. After 19 h of IVM, oocytes were exposed to demecolcine (0.05 µg mL?1) for 2 h, and enucleation was performed in oocytes that presented membrane protrusions. Samples of cytoplasts were stained with Hoechst 33342 for 10 min (10 µg mL?1) for evaluation of enucleation effectiveness. The remaining oocytes were reconstituted by NT, fused (two pulses of 2.0 kV cm?1 for 20 µs each, in 0.28 m mannitol solution), chemically activated (5 mm ionomycin for 5 min and 2 mm 6-DMAP for 4 h), and cultured in SOF medium with 2.5% FCS and 0.5% BSA. The 0.05 µg mL?1 concentration resulted in greater protrusion rates (55.1%; 211/388) in comparison to 0, 0.025, 0.2, and 0.4 µg mL?1 concentrations (0, 42.6, 45.1, and 39.3%, respectively). In Experiment II, at the beginning of treatment, the majority of the oocytes were in MII (40.7%) or anaphase I/telophase I (AI/TI; 22.4%). Effects of demecolcine occurred within only 0.5 h of treatment, with a significant increase in oocytes with complete depletion of microtubules (21.8%; initial average: 1.9%) and a reduction in the proportion at MII (13.5%) and AI/TI (8.2%). New polymerization of microtubules was observed when treated oocytes were then cultured in drug-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). In Experiment III, we verified the effectiveness of the chemically assisted enucleation technique (90.6%; 77/85). We evaluated 515 oocytes, of which 219 (42.5%) had protrusions and were enucleated. After losses in the NT procedure and fusion, 58 reconstructed 1-cells were cultured resulting in cleavage and blastocyst rates of 84.5% (49/58) and 27.6% (16/58), respectively, with great variation in blastocyst production between the three replicates (12.5% to 47%). In conclusion, demecolcine can be used at lower concentrations than those used routinely, and the chemically assisted enucleation method has proven highly efficient and does not appear to inhibit embryonic development in the bovine
650 $aoocytes
650 $aBOVINO
700 1 $aPERECIN, F.
700 1 $aMÉO, S. C.
700 1 $aFERREIRA, C. R.
700 1 $aTETZNER, T. A. D.
700 1 $aOLIVEIRA, J. C.
700 1 $aGARCIA, J. M.
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