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Registros recuperados : 233 | |
85. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | DUQUE, F. F.; DIDONET, A. D.; DOBEREINER, J. Selecao de estirpes de Rhizobium japonicum visando maior eficiencia na fixacao biologica do nitrogenio e na competitividade e sobrevivencia no solo. In; Seminario Nacional de Pesquisa de soja, 2, Brasilia, 1981. Anais... Londrina, EMBRAPA-CNPSo, 1982., v.2, p.752-765, 1982. Biblioteca(s): Embrapa Agrobiologia. |
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88. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MEDEIROS, D. S.; STONE, L. F.; DIDONET, A. D.; FERREIRA, E. P. B. Enzyme activity as quality indicator of soil under agroforestry systems in the Brazilian Cerrado. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FOREST SYSTEMS; INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 3., 2015, Brasília, DF. Towards sustainable intensification: proceedings. Brasília, DF: Embrapa, 2015. Biblioteca(s): Embrapa Arroz e Feijão. |
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89. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | COELHO, L. H.; FERREIRA, E. P. B.; DIDONET, C. C. G. M.; DIDONET, A. D. Desenvolvimento de cultivares de arroz inoculadas com bactérias promotoras de crescimento de plantas. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: anais. Londrina: Embrapa Soja: SBCS: IAPAR, UEL, 2008. 1 CD-ROM. Biblioteca(s): Embrapa Arroz e Feijão. |
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90. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | COELHO, L. H.; FERREIRA, E. P. B.; DIDONET, C. C. G. M.; DIDONET, A. D. Desenvolvimento de cultivares de arroz inoculadas com bactérias promotoras de crescimento de plantas. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: resumos. Londrina: Embrapa Soja: SBCS: IAPAR, UEL, 2008. Resumo 144. Biblioteca(s): Embrapa Arroz e Feijão. |
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92. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | RODRIGUES, O.; LHAMBY, J. C. B.; DIDONET, A. D.; MARCHESE, J. A. Fifty years of wheat breeding in Southern Brazil: yield improvement and associated changes. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 6, p. 817-825, jun. 2007. Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Trigo; Embrapa Unidades Centrais. |
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97. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | DIDONET, A. D.; RODRIGUES, O.; MARIO, J. L.; IDE, F.; TISOT, D. Temperatura base para os subperíodos de desenvolvimento de híbridos de milho. In: REUNIÃO TÉCNICA ANUAL DO MILHO, 43.; REUNIÃO TÉCNICA ANUAL DO SORGO, 26., 1998, Veranópolis. Anais... Veranópolis: Prefeitura Municipal / FEPAGRO / EMATER/RS: FECOAGRO, 1998. p. 100-103 Biblioteca(s): Embrapa Trigo. |
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Registros recuperados : 233 | |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
04/09/2015 |
Data da última atualização: |
20/06/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
FONSECA, F. C. de A.; FIRMINO, A. A. P.; MACEDO. L. L. P. de; COELHO, R. R.; SOUSA JÚNIOR, J. D. A. de; SILVA JUNIOR, O. B.; TOGAWA, R. C.; PAPPAS JUNIOR, G. J.; GÓIS, L. A. B. de; SILVA, M. C. M. da; SA, M. F. G. de. |
Afiliação: |
FERNANDO CAMPOS DE ASSIS FONSECA, UnB; ALEXANDRE AUGUSTO PEREIRA FIRMINO, UnB; LEONARDO LIMA PEPINO DE MACEDO, UnB; ROBERTA RAMOS COELHO; JOSÉ DIJAIR ANTONINO DE SOUSA JÚNIOR, UnB; ORZENIL BONFIM SILVA-JUNIOR, UnB; ROBERTO COITI TOGAWA, CENARGEN; GEORGIOS JOANNIS PAPPAS JUNIOR, Unb; LUIZ AVELAR BRANDÃO DE GÓIS, USINA TRUNFO, ALAGOAS; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN. |
Título: |
Sugarcane giant borer transcriptome analysis and identification of genes related to digestion. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Plos One, fev., 2015. |
DOI: |
10.1371/journal.pone.0118231 |
Idioma: |
Inglês |
Conteúdo: |
Sugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect?s biology and to guide the development of new strategies for insect-pest control. MenosSugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST d... Mostrar Tudo |
Thesagro: |
Broca Gigante; Controle Biológico; Praga. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129096/1/journal.pone.0118231.pdf
|
Marc: |
LEADER 02579naa a2200289 a 4500 001 2023363 005 2024-06-20 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0118231$2DOI 100 1 $aFONSECA, F. C. de A. 245 $aSugarcane giant borer transcriptome analysis and identification of genes related to digestion.$h[electronic resource] 260 $c2015 520 $aSugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect?s biology and to guide the development of new strategies for insect-pest control. 650 $aBroca Gigante 650 $aControle Biológico 650 $aPraga 700 1 $aFIRMINO, A. A. P. 700 1 $aMACEDO. L. L. P. de 700 1 $aCOELHO, R. R. 700 1 $aSOUSA JÚNIOR, J. D. A. de 700 1 $aSILVA JUNIOR, O. B. 700 1 $aTOGAWA, R. C. 700 1 $aPAPPAS JUNIOR, G. J. 700 1 $aGÓIS, L. A. B. de 700 1 $aSILVA, M. C. M. da 700 1 $aSA, M. F. G. de 773 $tPlos One, fev., 2015.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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