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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
10/01/2010 |
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Data da última atualização: |
12/09/2013 |
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Autoria: |
ZHU, C.; LIU, H. T.; DU, R. H. |
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Afiliação: |
Zhu Cheng; Liu Hou-tian; Du Ru-hui. |
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Título: |
Microphotometric determination of DNA contents of early developmental pollen grains in tobacco anther culture. |
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Ano de publicação: |
1982 |
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Fonte/Imprenta: |
Acta Botanica Sinica, Peking, v. 24, n. 1, p. 1-9, 1982. |
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Idioma: |
Chinês |
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Conteúdo: |
The Feulgen-DNA contents of microspores, vegetative and generative nuclei of tobacco pollen grains in vivo and in anther culture have been determined by microphotometry. 1. The values of DNA content of vegetative and generative nuclei of the pollen grains selected at definite developmental stages vary between 1C and 2C levels, which coincide with the role of the dynamics of DNA in haploid cell cycle. This method applied in the study of androgenesis in anther culture is proved successful and valid. 2. By the cytomorphological investigation on androgenesis, the pollen embryoid in this experiment results from repeated divisions of the vegetative cell within the pollen grains. 3. In mature pollen grains of the same variety of tobacco in vivo, DNA replication has not occured in vegetative nuclei, in which the level of DNA remains in 1C. 4. In the cultured anthers after 8 days innoculation, 30% of the total pollen grains measured indicate that the vegetative nuclei have completed DNA replication and show 2C level. The pollen grains which have the potential to differentiate into the embryogenie pollen grains, may be distinguished from non-embryogenie ones by this method before any cytomorphological sign appears. The significance of this method in the study of the mechanism of androgenesis is discussed. |
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Palavras-Chave: |
Determinação de DNA. |
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Thesagro: |
Pólen. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01814naa a2200169 a 4500
001 1837704
005 2013-09-12
008 1982 bl --- 0-- u #d
100 1 $aZHU, C.
245 $aMicrophotometric determination of DNA contents of early developmental pollen grains in tobacco anther culture.
260 $c1982
520 $aThe Feulgen-DNA contents of microspores, vegetative and generative nuclei of tobacco pollen grains in vivo and in anther culture have been determined by microphotometry. 1. The values of DNA content of vegetative and generative nuclei of the pollen grains selected at definite developmental stages vary between 1C and 2C levels, which coincide with the role of the dynamics of DNA in haploid cell cycle. This method applied in the study of androgenesis in anther culture is proved successful and valid. 2. By the cytomorphological investigation on androgenesis, the pollen embryoid in this experiment results from repeated divisions of the vegetative cell within the pollen grains. 3. In mature pollen grains of the same variety of tobacco in vivo, DNA replication has not occured in vegetative nuclei, in which the level of DNA remains in 1C. 4. In the cultured anthers after 8 days innoculation, 30% of the total pollen grains measured indicate that the vegetative nuclei have completed DNA replication and show 2C level. The pollen grains which have the potential to differentiate into the embryogenie pollen grains, may be distinguished from non-embryogenie ones by this method before any cytomorphological sign appears. The significance of this method in the study of the mechanism of androgenesis is discussed.
650 $aPólen
653 $aDeterminação de DNA
700 1 $aLIU, H. T.
700 1 $aDU, R. H.
773 $tActa Botanica Sinica, Peking$gv. 24, n. 1, p. 1-9, 1982.
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