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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
11/03/2014 |
Data da última atualização: |
12/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TIZIOTO, P.; GASPARINI, G.; SOUZA, M. M.; MUDADU, M. de A.; COUTINHO, L. L.; MOURÃO, G. B.; THOLON, P.; MEIRELLES, S. L. C.; TULLIO, R. R.; ROSA, A. do N.; ALENCAR, M. M. de; MEDEIROS, S. R.; SIQUEIRA, F.; FEIJO, G. L. D.; NASSU, R. T.; REGITANO, L. C. de A. |
Afiliação: |
POLIANA TIZIOTO, Universidade Federal de São Carlos; GUSTAVO GASPARINI, POS-GRADUAÇÃO UFSCar/SÃO CARLOS, SP; MARCELA M. SOUZA, POS-GRADUAÇÃO UFSCar/SÃO CARLOS, SP; MAURICIO DE ALVARENGA MUDADU, CPPSE; LUIZ L. COUTINHO, PROF. ESALQ/PIRACICABA; GERSON B. MOURÃO, ESALQ/PIRACICABA; PATRICIA THOLON, CPPSE; SARAH L. C. MEIRELLES, PROF. UFLA/LAVRAS; RYMER RAMIZ TULLIO, CPPSE; ANTONIO DO NASCIMENTO ROSA, CNPGC; MAURICIO MELLO DE ALENCAR, CPPSE; SERGIO R. MEDEIROS, CAMPO GRANDE; FABIANE SIQUEIRA, CNPGC; GELSON LUIS DIAS FEIJO, CNPGC; RENATA TIEKO NASSU, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
Título: |
Identification of KCNJ11 as a functional candidate gene for bovine meat tenderness. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Physiological Genomics, v. 45, n. 24, p. 1215-1221, 2013. |
DOI: |
10.1152/physiolgenomics.00137.2012 |
Idioma: |
Inglês |
Conteúdo: |
The potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P ? 0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P ? 0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, which in turn influence the flow of potassium in the intracellular space, allowing establishment of the membrane potential necessary for muscle contraction. MenosThe potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P ? 0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P ? 0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, whic... Mostrar Tudo |
Palavras-Chave: |
Bovine; Candidate gene; Differential expression; Molecular markers; Shear force; Warner Bratzler. |
Thesaurus Nal: |
beef; meat. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/108204/1/1215.full.pdf
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Marc: |
LEADER 02795naa a2200409 a 4500 001 1982035 005 2023-05-12 008 2013 bl uuuu u00u1 u #d 024 7 $a10.1152/physiolgenomics.00137.2012$2DOI 100 1 $aTIZIOTO, P. 245 $aIdentification of KCNJ11 as a functional candidate gene for bovine meat tenderness.$h[electronic resource] 260 $c2013 520 $aThe potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P ? 0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P ? 0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, which in turn influence the flow of potassium in the intracellular space, allowing establishment of the membrane potential necessary for muscle contraction. 650 $abeef 650 $ameat 653 $aBovine 653 $aCandidate gene 653 $aDifferential expression 653 $aMolecular markers 653 $aShear force 653 $aWarner Bratzler 700 1 $aGASPARINI, G. 700 1 $aSOUZA, M. M. 700 1 $aMUDADU, M. de A. 700 1 $aCOUTINHO, L. L. 700 1 $aMOURÃO, G. B. 700 1 $aTHOLON, P. 700 1 $aMEIRELLES, S. L. C. 700 1 $aTULLIO, R. R. 700 1 $aROSA, A. do N. 700 1 $aALENCAR, M. M. de 700 1 $aMEDEIROS, S. R. 700 1 $aSIQUEIRA, F. 700 1 $aFEIJO, G. L. D. 700 1 $aNASSU, R. T. 700 1 $aREGITANO, L. C. de A. 773 $tPhysiological Genomics$gv. 45, n. 24, p. 1215-1221, 2013.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
14/10/1998 |
Data da última atualização: |
19/12/2018 |
Autoria: |
SPERRY, S.; MAGNANI, M.; FREIRE, C. J. da S. |
Título: |
Bibliografia sobre analise foliar em frutiferas-maca. |
Ano de publicação: |
1981 |
Fonte/Imprenta: |
Pelotas: EMBRAPA-UEPAE de Cascata, 1981. |
Páginas: |
76p. |
Série: |
(EMBRAPA-UEPAE de Cascata. Documentos, 8). |
Idioma: |
Português |
Palavras-Chave: |
Fertilização; Ma?Æ. |
Thesagro: |
Análise Foliar. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00504nam a2200181 a 4500 001 1743135 005 2018-12-19 008 1981 bl uuuu u0uu1 u #d 100 1 $aSPERRY, S. 245 $aBibliografia sobre analise foliar em frutiferas-maca. 260 $aPelotas: EMBRAPA-UEPAE de Cascata$c1981 300 $a76p. 490 $a(EMBRAPA-UEPAE de Cascata. Documentos, 8). 650 $aAnálise Foliar 653 $aFertilização 653 $aMa?Æ 700 1 $aMAGNANI, M. 700 1 $aFREIRE, C. J. da S.
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