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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
09/06/2009 |
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Data da última atualização: |
27/11/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
VOLL, E.; GAZZIERO, D. L. P.; ADEGAS, F. S. |
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Afiliação: |
ELEMAR VOLL, CNPSo; DIONISIO LUIZ PISA GAZZIERO, CNPSo; FERNANDO STORNIOLO ADEGAS, CNPSo. |
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Título: |
Ácido aconítico em sementes de amendoim-bravo e corda-de-viola de diferentes locais. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: REUNIÃO DE PESQUISA DA SOJA DA REGIÃO SUL, 37., Porto Alegre, 2009. Programa e resumos. Porto Alegre : UFRGS, 2009. 1 CD-ROM. |
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Idioma: |
Português |
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Thesagro: |
Alelopatia; Erva Daninha; Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00561naa a2200169 a 4500
001 1466026
005 2009-11-27
008 2009 bl uuuu u00u1 u #d
100 1 $aVOLL, E.
245 $aÁcido aconítico em sementes de amendoim-bravo e corda-de-viola de diferentes locais.
260 $c2009
650 $aAlelopatia
650 $aErva Daninha
650 $aSoja
700 1 $aGAZZIERO, D. L. P.
700 1 $aADEGAS, F. S.
773 $tIn: REUNIÃO DE PESQUISA DA SOJA DA REGIÃO SUL, 37., Porto Alegre, 2009. Programa e resumos. Porto Alegre : UFRGS, 2009. 1 CD-ROM.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
05/12/2016 |
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Data da última atualização: |
05/12/2016 |
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Autoria: |
KREUTZ, L. C.; CANOVA, R.; NIED, C. O.; BORTOLUZZI, M.; FRANDOLOSO, R. |
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Afiliação: |
LUIZ C. KREUTZ, UPF; RAÍSSA CANOVA, UPF; CRISTIAN O. NIED, UPF; MÁRCIA BORTOLUZZI, UPF; RAFAEL FRANDOLOSO, UPF. |
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Título: |
Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF. V. 36, N. 9, p. 819-825, set. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Knowledge on fish immunoglobulin (Ig) characteristics and the availability of monoclonal or polyclonal antibodies to fish Igs are essential to evaluate the humoral immune response and the Ig distribution on leukocyte cells. We demonstrated that silver catfish serum Ig is composed of one immunodominant H chain with approximately 75k Da and one L chain with approximately 28 kDa, similar to human IgM. Rabbit polyclonal antibodies to the catfish IgM-like Ig recognized both the H and L chain and were useful in developing an indirect ELISA to measure the production of antibodies in fish immunized with bovine serum albumin. Dot blot and western blot cross-reactivity studies indicated a wide degree of epitope sharing amongst Ig from several Siluriformes and Characiformes fish indigenous to Brazilian rivers. In these fish species, polyclonal antibodies reacted mostly with the H chain. The results presented here are central to the development of tools and strategies to investigate the antibody production to inoculated antigens and tissue distribution of Ig molecules in native fish species. Furthermore, because of the wide range of cross-reactivity, polyclonal antibodies to silver catfish IgM-like Ig might be used to develop immunoassays to measure the humoral immune response in other fish species. |
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Palavras-Chave: |
Ensaio imunoenzimático; Produção anticorpos; Produção de anticorpos. |
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Thesagro: |
Bagre; Produção; Rhamdia Quelen. |
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Thesaurus NAL: |
Antibody formation; Catfish; Immunoassays; Immunoglobulin M; Polyclonal antibodies. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/151242/1/Characterization-of-an-IgM-like.pdf
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Marc: |
LEADER 02269naa a2200301 a 4500
001 2057948
005 2016-12-05
008 2016 bl uuuu u00u1 u #d
100 1 $aKREUTZ, L. C.
245 $aCharacterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays.
260 $c2016
520 $aKnowledge on fish immunoglobulin (Ig) characteristics and the availability of monoclonal or polyclonal antibodies to fish Igs are essential to evaluate the humoral immune response and the Ig distribution on leukocyte cells. We demonstrated that silver catfish serum Ig is composed of one immunodominant H chain with approximately 75k Da and one L chain with approximately 28 kDa, similar to human IgM. Rabbit polyclonal antibodies to the catfish IgM-like Ig recognized both the H and L chain and were useful in developing an indirect ELISA to measure the production of antibodies in fish immunized with bovine serum albumin. Dot blot and western blot cross-reactivity studies indicated a wide degree of epitope sharing amongst Ig from several Siluriformes and Characiformes fish indigenous to Brazilian rivers. In these fish species, polyclonal antibodies reacted mostly with the H chain. The results presented here are central to the development of tools and strategies to investigate the antibody production to inoculated antigens and tissue distribution of Ig molecules in native fish species. Furthermore, because of the wide range of cross-reactivity, polyclonal antibodies to silver catfish IgM-like Ig might be used to develop immunoassays to measure the humoral immune response in other fish species.
650 $aAntibody formation
650 $aCatfish
650 $aImmunoassays
650 $aImmunoglobulin M
650 $aPolyclonal antibodies
650 $aBagre
650 $aProdução
650 $aRhamdia Quelen
653 $aEnsaio imunoenzimático
653 $aProdução anticorpos
653 $aProdução de anticorpos
700 1 $aCANOVA, R.
700 1 $aNIED, C. O.
700 1 $aBORTOLUZZI, M.
700 1 $aFRANDOLOSO, R.
773 $tPesquisa Veterinária Brasileira, Brasília, DF. V. 36, N. 9, p. 819-825, set. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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