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Registros recuperados : 182 | |
82. | | DODE, M. A. N.; CAPOBIANCO, N.; VARGAS, L. N.; MION, B.; KUSSANO, N. R.; SPRICIGO, J. F.; FRANCO, M. M. Seminal cell–free DNA as a potential marker for in vitro fertility of Nellore bulls. Journal of Assisted Reproduction and Genetics, v. 41, p. 1357-1370, 2024. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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83. | | PAULINI, F.; FERREIRA, A. R.; MOURA, M. T.; RUA, P. J. S.; FRANCO, M. M.; MELO, E. de O. Production of GDF9 and BMP15 bovine hormones in homologue culture system. In: INTERNATIONAL SYMPOSIUM ON ANIMAL BIOLOGY OF REPRODUCTIONS, 3., 2010, São Pedro. Focus on Epigenetics. São Paulo: University of São Paulo, 2010. Pôster. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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84. | | MACHADO, G. M.; LAENDER, C. R.; FRANCO, M. M.; LEME, L. O.; RUMPF, R.; DODE, M. A. N. Post-hatching bovine embryo development in vitro: relationship between sex and size. Reproduction, Fertility and Development, v. 20, n. 1, p. 146, 2008. Edição dos proceedings of the Annual Conference of the International, Embryo Society, Denver, Colorado, 2008. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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86. | | FRANCO, M. M.; SANTOS, J. B. F.; MENDONÇA, A. S.; SILVA, T. C. F.; ANTUNES, R. C.; MELO, E. O. Quick method for identifying horse (Equus caballus) and donkey (Equus asinus) hybrids. Genetics and Molecular Research, v. 15, n. 33, article gmr.15038895, 2016. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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87. | | ARAÚJO, V. G. B.; PAULINI, F.; OLIVEIRA, G. S.; RUA, P. J. S.; FRANCO, M. M.; MELO, E. de O. Produção de anticorpos específicos para os hormônios GDF9 e BMP15 de bovinos. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 212. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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89. | | SILVEIRA, M. M.; BAYÃO, H.; MENDONÇA, A. S.; BORGES, N. A.; VARGAS, L. N.; RUMPF, R.; FRANCO, M. M. DNA methylation profile at a bovine alpha satellite region in placenta of a cloned animal. In: CONGRESSO BRASILEIRO DE GENÉTICA, 62., 2016, Caxambu. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2016. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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90. | | SILVEIRA, M. M.; BAYÃO, H.; MENDONÇA, A. S.; BORGES, N. A.; VARGAS, L. N.; RUMPF, R.; FRANCO, M. M. DNA methylation profile at a bovine testis satellite region is associated with aberrant placentation in cloned calves. In: BRAZILIAN INTERNATIONAL CONGRESS OF GENETICS, 2017, Águas de Lindóia, 12 a 15 set. 2017. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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93. | | PAULINI, F.; SOUZA, A. P. D. B.; VALERIANO, A. C. M.; CASTRO, E. A.; FRANCO, M. M.; MELO, E. O. Identificação de polimorfismos no gene BMP-15 em bovinos. Acta Scientiae Veterinariae, v. 36, supl. 2, p. S561, 2008. Edição dos resumos da 22. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, SP, 2008. Apresentado também no: In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília,... Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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94. | | SOUZA, A. P. D. B.; PAULINI, F.; VALERIANO, A. C. M.; CASTRO, E. A.; FRANCO, M. M.; MELO, E. O. Identificação de polimorfismos nos genes BMP-15 e GDF-9 em ovinos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 038. p. 78. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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97. | | VARGAS, L. N.; LEME, L. O.; SILVEIRA, M. M.; BAYÃO, H.; MENDONÇA, A. S.; RUMPF, R.; FRANCO, M. M. Transcriptional profile of methyltransferase enzymes in umbilical cord of cloned cattle. In: BRAZILIAN INTERNATIONAL CONGRESS OF GENETICS, 2017, Águas de Lindóia, 12 a 15 set. 2017. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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100. | | SIQUEIRA FILHO, E.; CAIXETA, E. S.; PRIBENSZKY, C.; MOLNAR, M.; HORVATH, A.; HARNOS, A.; FRANCO, M. M.; RUMPF, R. Vitrification of bovine blastocysts pretreated with sublethal hydrostatic pressure stress: evaluation of post-thaw in vitro development and gene expression. Reproduction, Fertility and Development, v. 23, p. 585-590, 2011. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 182 | |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
22/02/2013 |
Data da última atualização: |
07/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MACHADO, G. M.; CAIXETA, E. S.; LUCCI, C. M.; RUMPF, R.; FRANCO, M. M.; DODE, M. A. N. |
Afiliação: |
GRAZIELI MARINHEIRO MACHADO, UnB; ESTER SIQUEIRA CAIXETA, UnB; CAROLINA MADEIRA LUCCI, UnB; RODOLFO RUMPF, CENARGEN; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
Título: |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Zygote, v. 20, p. 123-134, 2011. |
Idioma: |
Inglês |
Conteúdo: |
The objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression. MenosThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demon... Mostrar Tudo |
Palavras-Chave: |
Cultura do embrião; Sexagem. |
Thesagro: |
Gado. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179338/1/posthatching-development-of-bovine-embryos-in-vitro-the-effects-of-tunnel-preparation-and-gender.pdf
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Marc: |
LEADER 02172naa a2200217 a 4500 001 1950734 005 2023-03-07 008 2011 bl uuuu u00u1 u #d 100 1 $aMACHADO, G. M. 245 $aPost-hatching development of bovine embryos in vitro$bthe effects of tunnel preparation and gender.$h[electronic resource] 260 $c2011 520 $aThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression. 650 $aGado 653 $aCultura do embrião 653 $aSexagem 700 1 $aCAIXETA, E. S. 700 1 $aLUCCI, C. M. 700 1 $aRUMPF, R. 700 1 $aFRANCO, M. M. 700 1 $aDODE, M. A. N. 773 $tZygote$gv. 20, p. 123-134, 2011.
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