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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
22/12/2008 |
Data da última atualização: |
22/11/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ETCHEGARAY, A.; BUENO, C. de C.; MELO, I. S. de; TSAI, S. M.; FIORE, M. F.; SILVA-STENICO, M. E.; MORAES, L. A. B. de; TESCHKE, O. |
Afiliação: |
Augusto Etchegaray, Faculdade de Química, PUC-Campinas; Carolina de Castro Bueno, Faculdade de Química, PUC-Campinas; ITAMAR SOARES DE MELO, CNPMA; S. M. TSAI, CENA/USP; M. F. FIORE, CENA/USP; M. E. SILVA-STENICO, CENA/USP; L. A. B. de MORAES, Departamento de Química/USP; O. TESCHKE, Laboratório de Nanoestruturas e Interfaces, Instituto de Física, UNICAMP. |
Título: |
Effect of a highly concentrated lipopeptide extract of Bacillus subtilis on fungal and bacterial cells. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Archives of Microbiology, v. 190, n. 6, p.611-622, 2008. |
Idioma: |
Inglês |
Conteúdo: |
Lipopeptides produced by Bacillus subtilis are known for their high antifungal activity. The aim of this paper is to show that at high concentration they can damage the surface ultra-structure of bacterial cells. A lipopeptide extract containing iturin and surfactin (5 mg mL¡1) was prepared after isolation from B. subtilis (strain OG) by solid phase extraction. Analysis by atomic force microscope (AFM) showed that upon evaporation, lipopeptides form large aggregates (0.1?0.2 m2) on the substrates silicon and mica. When the same solution is incubated with fungi and bacteria and the system is allowed to evaporate, dramatic changes are observed on the cells. AFM micrographs show disintegration of the hyphae of Phomopsis phaseoli and the cell walls of Xanthomonas campestris and X. axonopodis. Collapses to fungal and bacterial cells may be a result of formation of pores triggered by micelles and lamellar structures, which are formed above the critical micelar concentration of lipopeptides. As observed for P. phaseoli, the process involves binding, solubilization, and formation of novel structures in which cell wall components are solubilized within lipopeptide vesicles. This is the Wrst report presenting evidences that vesicles of uncharged and negatively charged lipopeptides can alter the morphology of gramnegative bacteria |
Thesagro: |
Controle biológico; Fungo. |
Categoria do assunto: |
V Taxonomia de Organismos |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/150376/1/2008CP-07.pdf
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Marc: |
LEADER 02028naa a2200229 a 4500 001 1015579 005 2016-11-22 008 2008 bl uuuu u00u1 u #d 100 1 $aETCHEGARAY, A. 245 $aEffect of a highly concentrated lipopeptide extract of Bacillus subtilis on fungal and bacterial cells.$h[electronic resource] 260 $c2008 520 $aLipopeptides produced by Bacillus subtilis are known for their high antifungal activity. The aim of this paper is to show that at high concentration they can damage the surface ultra-structure of bacterial cells. A lipopeptide extract containing iturin and surfactin (5 mg mL¡1) was prepared after isolation from B. subtilis (strain OG) by solid phase extraction. Analysis by atomic force microscope (AFM) showed that upon evaporation, lipopeptides form large aggregates (0.1?0.2 m2) on the substrates silicon and mica. When the same solution is incubated with fungi and bacteria and the system is allowed to evaporate, dramatic changes are observed on the cells. AFM micrographs show disintegration of the hyphae of Phomopsis phaseoli and the cell walls of Xanthomonas campestris and X. axonopodis. Collapses to fungal and bacterial cells may be a result of formation of pores triggered by micelles and lamellar structures, which are formed above the critical micelar concentration of lipopeptides. As observed for P. phaseoli, the process involves binding, solubilization, and formation of novel structures in which cell wall components are solubilized within lipopeptide vesicles. This is the Wrst report presenting evidences that vesicles of uncharged and negatively charged lipopeptides can alter the morphology of gramnegative bacteria 650 $aControle biológico 650 $aFungo 700 1 $aBUENO, C. de C. 700 1 $aMELO, I. S. de 700 1 $aTSAI, S. M. 700 1 $aFIORE, M. F. 700 1 $aSILVA-STENICO, M. E. 700 1 $aMORAES, L. A. B. de 700 1 $aTESCHKE, O. 773 $tArchives of Microbiology$gv. 190, n. 6, p.611-622, 2008.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
31/01/2008 |
Data da última atualização: |
16/05/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Nacional - A |
Autoria: |
MELO, E. O.; CANAVESSI, A. M. O; FRANCO, M. M.; RUMPF, R. |
Afiliação: |
EDUARDO DE OLIVEIRA MELO, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; AUREA M. O. CANAVESSI; MAURICIO MACHAIM FRANCO, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; RODOLFO RUMPF, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA. |
Título: |
Animal transgenesis: state of the art and applications. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Journal of Applied Genetics, v. 48, n. 1, p. 47-61, 2007. |
Idioma: |
Inglês |
Palavras-Chave: |
Cloning. |
Thesagro: |
Animal. |
Thesaurus NAL: |
livestock. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156019/1/art3A10.10072FBF03194657.pdf
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Marc: |
LEADER 00504naa a2200181 a 4500 001 1189124 005 2024-05-16 008 2007 bl uuuu u00u1 u #d 100 1 $aMELO, E. O. 245 $aAnimal transgenesis$bstate of the art and applications.$h[electronic resource] 260 $c2007 650 $alivestock 650 $aAnimal 653 $aCloning 700 1 $aCANAVESSI, A. M. O 700 1 $aFRANCO, M. M. 700 1 $aRUMPF, R. 773 $tJournal of Applied Genetics$gv. 48, n. 1, p. 47-61, 2007.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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