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Registros recuperados : 183 | |
161. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CARVALHO, J. O.; MICHALCZECHEN-LACERDA, V. A.; SARTORI, R.; RODRIGUES, F. C.; BRAVIM, O.; FRANCO, M. M.; DODE, M. A. N. The methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting. Molecular Reproduction e Development, v. 79, p. 77-84, 2012. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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162. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FERNANDES, G. de O.; MILAZZOTTO, M. P.; FIDELIS, A. A. G.; KAWAMOTO, T. S.; LEME, L. de O.; LIMA, C. B. de; FRANCO, M. M.; DODE, M. A. N. Biochemical markers for pregnancy in the spent culture medium of in vitro produced bovine embryos. Biology of Reproduction, v. 105, n. 2, 481-490, 2021. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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163. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | RODRIGUES, S. A. D.; PONTELO, T. P.; KUSSANO, N. R.; KAWAMOTO, T. S.; LEME, L. O.; CAIXETA, F. M. C.; PFEIFER, L. F. M.; FRANCO, M. M.; DODE, M. A. N. Effects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes. Domestic Animal Endocrinology, v. 72, article 106447, 2020. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia. |
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164. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SPRÍCIGO, J. F. W.; SENA NETTO, S. B.; MUTERLLE, C. V.; RODRIGUES, S. de A. D.; LEME, L. O.; GUIMARÃES, A. L.; CAIXETA, F. M. C.; FRANCO, M. M.; PIVATO, I.; DODE, M. A. N. Intrafollicular transfer of fresh and vitrified immature bovine oocytes. Theriogenology, v. 86, p. 2054-2062, 2016. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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165. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CÁCERES, M. B. S.; SILVA, W. A. L. da; LIMA, A. C. B. de; OLIVEIRA JR, J. S. de; CARDOSO, C. J. T.; SANTOS, J. V. dos; ANDRADE, E. R.; FRANCO, M. M.; POEHLAND, R.; STERZA, F. de A. M. Trimethylation of histone 3 at lysine 4 in cryopreserved bovine embryos produced in vivo with sexed semen. Theriogenology, v. 86, p. 1944-1952, 2016. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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166. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SILVA, C. G. da; MARTINS, C. F.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; CARDOSO, T. C.; FRANCO, M. M.; MENDONÇA, A. dos S.; LEME, L. de O.; BORGES, J. R. J.; MALAQUIAS, J. V.; BAO, S. N. Use of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells. Reproduction in Domestic Animals, v. 54, n. 2, p. 289-299, February 2019. Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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167. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BRAGA, T. F.; SILVA, T. C. F.; MARQUES, M. G.; SOUZA, A. P. de; ALBRING, D.; SILVA, L. P.; CAETANO, A. R.; DODE, M. A. N.; FRANCO, M. M. The dynamics of gene expression, lipid composition and DNA methylation reprogramming are different during in vitro maturation of pig oocytes obtained from prepubertal gilts and cycling sows. Reproduction in Domestic Animals, v. 54, n. 9, p. 1217-1229, 2019. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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168. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | VARGAS, L. N.; NOCHI, A. R. F.; CASTRO, P. S. de; CUNHA, A. T. M.; SILVA, T. C. V.; TOGAWA, R. C.; SILVEIRA, M. M.; CAETANO, A. R.; FRANCO, M. M. Differentially methylated regions identified in bovine embryos are not observed in adulthood. Animal Reproduction, v. 20, n. 1, 2023. e20220076. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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169. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | IGUMA, L. T.; LISAUSKAS, S. F. C.; MELO, E. O.; FRANCO, M. M.; PIVATO, I.; VIANNA, G. R.; SOUSA, R. V.; DODE, M. A. N.; ARAGÃO, F. J. L.; RECH, E. L.; RUMPF, R. Development of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells. Genetics and Molecular Research, v. 4, n. 1, p. 55-66, 2005. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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170. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MACHADO, G. M.; FERREIRA, A. R.; GUARDIEIRO, M. M.; BASTOS, M. R.; CARVALHO, J. O.; LUCCI, C. M.; DIESEL, T. O.; SARTORI, R.; RUMPF, R.; FRANCO, M. M.; DODE, M. A. N. Morphology, sex ratio and gene expression of Day 14 in vivo and in vitro bovine embryos. Reproduction, Fertility and Development, v. 25, p. 600-608, 2013. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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171. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MARTINS, C. F.; FONSECA NETO, A. M. da; BESSLER, H. C.; DODE, M. A. N.; LEME, L. O.; FRANCO, M. M.; McMANUS, C. M.; MALAQUIAS, J. V.; FERREIRA, I. C. Natural shade from integrated crop livestock forestry mitigates environmental heat and increases the quantity and quality of oocytes and embryos produced in vitro by Gyr dairy cows. Livestock Science, 2020. Biblioteca(s): Embrapa Cerrados. |
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172. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MARTINS, C. F.; FONSECA NETO, A. M. da; BESSLER, H. C.; DODE, M. A. N.; LEME, L. O.; FRANCO, M. M.; McMANUS, C. M.; MALAQUIAS, J. V.; FERREIRA, I. C. Natural shade from integrated crop livestock forestry mitigates environmental heat and increases the quantity and quality of oocytes and embryos produced in vitro by Gyr dairy cows. Livestock Science, v. 244, 104341, 2021. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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173. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SILVA, B. D. M.; CASTRO, E. A.; SOUZA, C. J. H. de; PAIVA, S. R.; SARTORI, R.; FRANCO, M. M.; AZEVEDO, H. C.; SILVA, T. A. S. N.; VIEIRA, A. M. C.; NEVES, J. P.; MELO, E. de O. A new polymorphism in the Growth and Differentiation Factor 9 (GDF9) gene is associated with increased ovulation rate and prolificacy in homozygous sheep. Animal Genetics, Oxford, v. 42, n. 1, p. 89-92, Jan. 2011. Biblioteca(s): Embrapa Pecuária Sul; Embrapa Recursos Genéticos e Biotecnologia. |
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174. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MELO, E. O.; SILVA, B. D. M.; CASTRO, E. A.; SILVA, T. A. S. N.; PAIVA, S. R.; SARTORI, R.; FRANCO, M. M.; SOUZA, C. J. H. de; NEVES, J. P. A novel mutation in the growth and differentiation factor 9 (GDF9) gene is associated, in homozygosis, with increased ovulation rate in Santa Ines sheep. In: ANNUAL MEETING OF THE SOCIETY FOR THE STUDY OF REPRODUCTION, 41., 2008, Kailua-Kona. Reproductive biology: basic discoveries that affect our lives: electronic proceedings. Madison: SSR, 2008. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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175. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FAGUNDES, F. C.; MICHALCZECHEN LACERDA, V. A.; CAIXETA, E. S.; MACHADO, G. M.; RODRIGUES, F. C.; GOMES, L. F. S.; MELO, E. de O.; RUMPF, R.; DODE, M. A. N.; FRANCO, M. M. Padrão de metilação da DMR do último Éxon do gene IGF2 em ovócitos bovinos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 14., 2009, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2009. Resumo 045. p. 84 Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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176. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PRICKETT, A. R.; MONTIBUS, B.; BARKAS, N.; AMANTE, S. M.; FRANCO, M. M.; COWLEY, M.; PUSZYK, W.; SHANNON, M. F.; IRVING, M. D.; MADON-SIMON, M.; WARD, A.; SCHULZ, R.; BALDWIN, H. S.; OAKEY, R. J. Imprinted gene expression and function of the dopa decarboxylase gene in the developing heart. Frontiers in Cell and Developmental Biology, v. 9, article 676543, 2021. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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177. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SILVA, T. C. F.; DODE, M. A. N.; BRAGA, T. F.; MARQUES, M. G.; VARGAS, L. N.; FARIA, O. A. C. de; SOUZA, A. P. de; ALBRING, D.; CAETANO, A. R.; FRANCO, M. M. Cumulus-oocyte complexes from sows show differences in lipid metabolism compared to cumulus-oocyte complexes from prepubertal gilts during in vitro maturation. Molecular Reproduction & Development, v. 90, n. 5, p. 323-335. 2023. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Suínos e Aves. |
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178. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FERREIRA, I. M.; BRAGA, T. F.; GUIMARÃES, E. C.; DURVAL, M. C.; MENDES, L. B.; SOARES, J. S.; MATARIM, D. L.; KRUGER, B. C.; FRANCO, M. M.; ANTUNES, R. C. A DdeI polymorphism in the growth hormone gene is associated with higher lean meat yield and pH 45 min in postmortem pigs. Genetics and Molecular Research, v. 18, n. 2, article gmr18257, 2019. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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179. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | LEME, L. O.; MACHADO, G. M.; FIDELIS, A. A. G.; GUIMARÃES, A. L. S.; SPRÍCIGO, J. F. W.; CARVALHO, J. O.; PIVATO, I.; FRANCO, M. M.; DODE, M. A. N. Transcriptome of D14 in vivo x in vitro bovine embryos: is there any difference? In Vitro Cellular & Developmental Biology - Animal, v. 57, p. 598-609, 2021. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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180. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | IGUMA, L. T.; PIVATO, I.; MELO, L. F.; PEREIRA, D. C.; MACHADO, G. M.; MOSCARDINE, A. R. C.; GRATTAPAGLIA, D.; FERREIRA, M. E.; PALUDO, G. R.; REIS JUNIOR, J. L.; FRANCO, M. M.; BORGES, J. R. J.; RUMPF, R. Vitoriosa da Embrapa - primeiro clone do clone da América Latina. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 9., 2004, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. p. 188. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 183 | |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia. |
Data corrente: |
05/06/2020 |
Data da última atualização: |
15/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
RODRIGUES, S. A. D.; PONTELO, T. P.; KUSSANO, N. R.; KAWAMOTO, T. S.; LEME, L. O.; CAIXETA, F. M. C.; PFEIFER, L. F. M.; FRANCO, M. M.; DODE, M. A. N. |
Afiliação: |
SARAH A. D. RODRIGUES, Universidade de Brasília; THAIS P. PONTELO, Universidade Federal de Lavras; NAYARA R. KUSSANO, Universidade de Brasília; TAYNAN S. KAWAMOTO, Universidade Federal de Uberlândia; LIGIANE O. LEME, Universidade Federal do Espírito Santo; FELIPPE M. C. CAIXETA, Universidade de Brasília; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; MAURICIO MACHAIM FRANCO, Cenargen; MARGOT ALVES NUNES DODE, Cenargen. |
Título: |
Effects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Domestic Animal Endocrinology, v. 72, article 106447, 2020. |
ISSN: |
0739-7240 |
DOI: |
https://doi.org/10.1016/j.domaniend.2020.106447 |
Idioma: |
Inglês |
Conteúdo: |
We aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the expression of PGE2 and PGF2a receptor genes was either not detectable or was detected at low levels in oocytes. MenosWe aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the... Mostrar Tudo |
Palavras-Chave: |
E2; Embryonic development; F2a; Maturation; Oócito; Oocyte competence. |
Thesagro: |
Bovinocultura; Cultura In Vitro; Embriogénese; Maturação; Ovulo; Prostaglandina; Reprodução Animal. |
Thesaurus NAL: |
Animal reproduction; Cattle; Embryogenesis; Gene expression; In vitro studies; Oocytes; Prostaglandins. |
Categoria do assunto: |
-- K Ciência Florestal e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219006/1/cpafro-18481.pdf
|
Marc: |
LEADER 02942naa a2200481 a 4500 001 2127907 005 2021-09-15 008 2020 bl uuuu u00u1 u #d 022 $a0739-7240 024 7 $ahttps://doi.org/10.1016/j.domaniend.2020.106447$2DOI 100 1 $aRODRIGUES, S. A. D. 245 $aEffects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes.$h[electronic resource] 260 $c2020 520 $aWe aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the expression of PGE2 and PGF2a receptor genes was either not detectable or was detected at low levels in oocytes. 650 $aAnimal reproduction 650 $aCattle 650 $aEmbryogenesis 650 $aGene expression 650 $aIn vitro studies 650 $aOocytes 650 $aProstaglandins 650 $aBovinocultura 650 $aCultura In Vitro 650 $aEmbriogénese 650 $aMaturação 650 $aOvulo 650 $aProstaglandina 650 $aReprodução Animal 653 $aE2 653 $aEmbryonic development 653 $aF2a 653 $aMaturation 653 $aOócito 653 $aOocyte competence 700 1 $aPONTELO, T. P. 700 1 $aKUSSANO, N. R. 700 1 $aKAWAMOTO, T. S. 700 1 $aLEME, L. O. 700 1 $aCAIXETA, F. M. C. 700 1 $aPFEIFER, L. F. M. 700 1 $aFRANCO, M. M. 700 1 $aDODE, M. A. N. 773 $tDomestic Animal Endocrinology$gv. 72, article 106447, 2020.
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Embrapa Rondônia (CPAF-RO) |
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