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61. | | IGUMA, L. T.; PIVATO, I.; CÂMARA, J. U.; PEREIRA, D. C.; MUNDIM, T. C. D.; FRANCO, M. M.; RUMPF, R. Bovine cloning using recipient cytoplasts recovered from hormone-stimulated heifers. In: REUNIÓN ASOCIACÓN LATINOAMERICANA DE PRODUCCIÓN ANIMAL, 20.; REUNIÓN ASOCIACIÓN PERUANA DE PRODUCCIÓN ANIMAL, 30.; CONGRESO INTERNACIONAL DE GANADERIA DOBLE PROPOSITO, 5., 2007, Cuzco. Resumenes... Cuzco: ALPA/APPA, 2007. Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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62. | | LOPEZ, I. M. R.; SOUZA, C. J. H. de; FRANCO, M. M.; RUMPF, R.; MELO, E. O. Clonagem e expressão das regiões gênicas codificadoras do peptídeo maduro nos hormônios BMP15 e GDF9 de clones bovinos. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2005. p. 86. Biblioteca(s): Embrapa Pecuária Sul. |
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64. | | MELO, E. O.; SOUSA, R. V.; IGUMA, L. T.; FRANCO, M. M.; RECH, E. L.; RUMPF, R. Isolation of transfected fibroblast clones for use in nuclear transfer and transgene detection in cattle embryos. Genetics and Molecular Research, v. 4, n. 4, p. 812-821, 2005. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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65. | | COSTA, N. S.; SILVEIRA, M. M.; VARGAS, L. N.; CAETANO, A. R.; RUMPF, R.; FRANCO, M. M. Epigenetic characterization of the H19/IGF2 locus in calf clones placenta. Pesquisa Veterinária Brasileira, v. 40, n. 12, p. 1063-1072, 2020. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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69. | | SILVEIRA, M. M.; BAYÃO, H.; MENDONÇA, A. S.; BORGES, N. A.; VARGAS, L. N.; RUMPF, R.; FRANCO, M. M. DNA methylation profile at a bovine alpha satellite region in placenta of a cloned animal. In: CONGRESSO BRASILEIRO DE GENÉTICA, 62., 2016, Caxambu. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2016. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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70. | | SILVEIRA, M. M.; BAYÃO, H.; MENDONÇA, A. S.; BORGES, N. A.; VARGAS, L. N.; RUMPF, R.; FRANCO, M. M. DNA methylation profile at a bovine testis satellite region is associated with aberrant placentation in cloned calves. In: BRAZILIAN INTERNATIONAL CONGRESS OF GENETICS, 2017, Águas de Lindóia, 12 a 15 set. 2017. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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72. | | AZEVEDO, H. C.; OLIVEIRA, A. A. de; MUNIZ, E. N.; PAIVA, S. R.; FRANCO, M. M.; MELO, E. de O. Embrapa pesquisa ovelhas que produzem mais crias por parto. Zoonews, 5 fev. 2011. Disponível em: . Artigo na mídia. Disponível em: 5 fev. 2011, .; 5 fev. 2011, ; 5 fev. 2011, ; . Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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73. | | AZEVEDO, H. C.; OLIVEIRA, A. A. de; MUNIZ, E. N.; PAIVA, S. R.; FRANCO, M. M.; MELO, E. de O. Embrapa Tabuleiros Costeiros produz rebanho de ovelhas Santa Inês com maior prolificidade. Aracaju, Se, Embrapa Tabuleiros Costeiros, 22 out., 2010. Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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75. | | AZEVEDO, J. M.; FERREIRA, A. R.; MACHADO, G. M.; SARTORI, R.; DODE, M. A. N.; FRANCO, M. M. Expressão de genes relacionados à metilação do DNA e metabolismo de glicose em embriões bovinos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 044. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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76. | | CAIXETA, E. S.; FIGUEIREDO, P. R.; MACHADO, G. M.; FRANCO, M. M.; BURATINI, J.; DODE, A. N. Expression of candidate genes for oocyte competence in bovine Cumulus cells. Reproduction, Fertility and Development, v.21, n.1, p. 189, 2009. Edição dos proceedings da Annual Conference or the International Embryo Transfer Society, San Diego, California, 2009. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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77. | | FERREIRA, A. R.; MACHADO, G. M.; AZEVEDO, J. M.; SARTORI, R.; DODE, M. A. N.; FRANCO, M. M. Expression of genes related to DNA methylation and glucose metabolism during the pre-implantational stage of bovine embryos. Reproduction, Fertility and Development, v.23, n.1, p. 132-133, 2011. Edição dos proceedings da Annual Conference or the International Embryo Transfer Society, Orlando, Florida, 2011. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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78. | | MOLLO, M. R.; RAMOS, A. F.; PIVATO, I.; GUIMARÃES NETO, A. G.; FRANCO, M. M.; RUMPF, R.; SARTORI, R. Aumento de recuperação embrionária usando lavagem uterina dupla em bovinos, corroborando dados de um estudo anterior. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 101. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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79. | | MICHALCZECHEN LACERDA, V. A.; DRIESSEN, K.; FAGUNDES, N. S.; MELO, E. O.; RUMPF, R.; FRANCO, M. M. Análise de metilação de DNA em células doadoras de núcleo para clonagem de bovinos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 054. p. 96. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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80. | | SOUSA, R. V. de; CARDOSO, C. R. da S.; BUTZKE, G.; DODE, M. A. N.; RUMPF, R.; FRANCO, M. M. Biopsy of bovine embryos produced in vivo and in vitro does not affect pregnancy rates. Theriogenology, v. 90, n. 1, p. 25-31, 2017. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 182 | |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
22/03/2017 |
Data da última atualização: |
14/06/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SOUSA, R. V. de; CARDOSO, C. R. da S.; BUTZKE, G.; DODE, M. A. N.; RUMPF, R.; FRANCO, M. M. |
Afiliação: |
Regivaldo Vieira de Sousa, UnB; Célia Regina da Silva Cardoso, Guilberth Serviços Veterinários S / A; Guilberth Butzke, Guilberth Serviços Veterinários S/A; MARGOT ALVES NUNES DODE, Cenargen; Rodolfo Rumpf, Geneal Genética Animal–Análise, Pesquisa e Laboratório S/A; MAURICIO MACHAIM FRANCO, Cenargen. |
Título: |
Biopsy of bovine embryos produced in vivo and in vitro does not affect pregnancy rates. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Theriogenology, v. 90, n. 1, p. 25-31, 2017. |
Idioma: |
Inglês |
Conteúdo: |
Assisted reproductive techniques have significantly contributed to animal breeding programs. Similarly, genomics has provided important information and tools to improve the accuracy of selection. However, the greatest benefits of those tools can only be expected when they are combined, allowing animals to be selected accurately early in life. Therefore, obtaining DNA samples from embryos without compromising their viability is essential for the consolidation of preimplantation genomic selection. We aimed to evaluate the effect on the gestation rate of conducting a biopsy of in vivo (VV) and in vitro-produced (IVP) bovine embryos. The VV and IVP embryos were distributed into two groups: VV-B (biopsied embryos; n ¼ 380) and VV-C (intact embryos?controls; n ¼ 229) and IVP-B (biopsied embryos; n ¼ 91) and IVP-C (intact embryos?controls; n ¼ 227), respectively. After biopsy, embryos from both groups VV-B and IVP-B were cultured for an additional 3 hours before being transferred to synchronized recipients. To evaluate the quality of the DNA obtained in the biopsies, this was used to determine the sex of embryos by polymerase chain reaction. No effect (P > 0.05) of the biopsy was observed for any of the treatments, the pregnancy rate at D 60 post-transfer being similar for VV-B: 206/380 (54.21%) and VV-C: 128/229 (55.89%) and for IVP-B: 24/91 (26.37%) and IVP-C: 45/227 (19.82%). Also, no effect (P > 0.05) of the embryo?s stage of development was detected on percentage of pregnant recipients when in vitro embryos were transferred. From the biopsies analyzed, about 90% had the sex determined, confirming that DNA was there and it was efficiently amplified. The results indicated that biopsy does not affect the viability of IVV and IVP bovine embryos and can be used in commercial programs to associate assisted reproductive technologies with genomic selection. MenosAssisted reproductive techniques have significantly contributed to animal breeding programs. Similarly, genomics has provided important information and tools to improve the accuracy of selection. However, the greatest benefits of those tools can only be expected when they are combined, allowing animals to be selected accurately early in life. Therefore, obtaining DNA samples from embryos without compromising their viability is essential for the consolidation of preimplantation genomic selection. We aimed to evaluate the effect on the gestation rate of conducting a biopsy of in vivo (VV) and in vitro-produced (IVP) bovine embryos. The VV and IVP embryos were distributed into two groups: VV-B (biopsied embryos; n ¼ 380) and VV-C (intact embryos?controls; n ¼ 229) and IVP-B (biopsied embryos; n ¼ 91) and IVP-C (intact embryos?controls; n ¼ 227), respectively. After biopsy, embryos from both groups VV-B and IVP-B were cultured for an additional 3 hours before being transferred to synchronized recipients. To evaluate the quality of the DNA obtained in the biopsies, this was used to determine the sex of embryos by polymerase chain reaction. No effect (P > 0.05) of the biopsy was observed for any of the treatments, the pregnancy rate at D 60 post-transfer being similar for VV-B: 206/380 (54.21%) and VV-C: 128/229 (55.89%) and for IVP-B: 24/91 (26.37%) and IVP-C: 45/227 (19.82%). Also, no effect (P > 0.05) of the embryo?s stage of development was detected on percentage of pregnant r... Mostrar Tudo |
Palavras-Chave: |
Embryo viability; Identificação sexual; Seleção genômica; Sex identification; Viabilidade embrionária. |
Thesagro: |
Bovino. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02625naa a2200253 a 4500 001 2067481 005 2024-06-14 008 2017 bl uuuu u00u1 u #d 100 1 $aSOUSA, R. V. de 245 $aBiopsy of bovine embryos produced in vivo and in vitro does not affect pregnancy rates.$h[electronic resource] 260 $c2017 520 $aAssisted reproductive techniques have significantly contributed to animal breeding programs. Similarly, genomics has provided important information and tools to improve the accuracy of selection. However, the greatest benefits of those tools can only be expected when they are combined, allowing animals to be selected accurately early in life. Therefore, obtaining DNA samples from embryos without compromising their viability is essential for the consolidation of preimplantation genomic selection. We aimed to evaluate the effect on the gestation rate of conducting a biopsy of in vivo (VV) and in vitro-produced (IVP) bovine embryos. The VV and IVP embryos were distributed into two groups: VV-B (biopsied embryos; n ¼ 380) and VV-C (intact embryos?controls; n ¼ 229) and IVP-B (biopsied embryos; n ¼ 91) and IVP-C (intact embryos?controls; n ¼ 227), respectively. After biopsy, embryos from both groups VV-B and IVP-B were cultured for an additional 3 hours before being transferred to synchronized recipients. To evaluate the quality of the DNA obtained in the biopsies, this was used to determine the sex of embryos by polymerase chain reaction. No effect (P > 0.05) of the biopsy was observed for any of the treatments, the pregnancy rate at D 60 post-transfer being similar for VV-B: 206/380 (54.21%) and VV-C: 128/229 (55.89%) and for IVP-B: 24/91 (26.37%) and IVP-C: 45/227 (19.82%). Also, no effect (P > 0.05) of the embryo?s stage of development was detected on percentage of pregnant recipients when in vitro embryos were transferred. From the biopsies analyzed, about 90% had the sex determined, confirming that DNA was there and it was efficiently amplified. The results indicated that biopsy does not affect the viability of IVV and IVP bovine embryos and can be used in commercial programs to associate assisted reproductive technologies with genomic selection. 650 $aBovino 653 $aEmbryo viability 653 $aIdentificação sexual 653 $aSeleção genômica 653 $aSex identification 653 $aViabilidade embrionária 700 1 $aCARDOSO, C. R. da S. 700 1 $aBUTZKE, G. 700 1 $aDODE, M. A. N. 700 1 $aRUMPF, R. 700 1 $aFRANCO, M. M. 773 $tTheriogenology$gv. 90, n. 1, p. 25-31, 2017.
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