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Registro Completo |
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
12/01/2024 |
Data da última atualização: |
12/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BRIGIDA, A. I. S.; BUARQUE, F. S.; NOGUEIRA, V. L. R.; MELO, V. M. M.; GUISÁN SEIJAS, J. M.; RIBEIRO, B. D.; GONÇALVES, L. R. B.; COELHO, M. A. Z. |
Afiliação: |
ANA IRAIDY SANTA BRIGIDA, CNPAT; FILIPE SMITH BUARQUE, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; VANESSA LÚCIA RODRIGUES NOGUEIRA, UNIVERSIDADE FEDERAL DO CEARÁ; VÂNIA MARIA MACIEL MELO, UNIVERSIDADE FEDERAL DO CEARÁ; JOSÉ MANUEL GUISÁN SEIJAS, INSTITUTE OF CATALYSIS AND PETROCHEMISTRY; BERNARDO DIAS RIBEIRO, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; LUCIANA ROCHA BARROS GONÇALVES, UNIVERSIDADE FEDERAL DO CEARÁ; MARIA ALICE ZARUR COELHO, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
Título: |
Partial purification of crude lipase extract from Yarrowia lipolytica: precipitation, aqueous two-phase systems (ATPS), and immobilization methods. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Cleaner Chemical Engineering, v. 6, 11 p., art. 100105, June 2023. |
DOI: |
10.1016/j.clce.2023.100105 |
Idioma: |
Inglês |
Conteúdo: |
Efforts have been concentrated on developing alternative methods of enzyme purification that are less costly and highly efficient. In this work, we evaluated three different methods for lipase purification from Yarrowia lipolytica, such as precipitation using ammonium sulfate, ethanol, or acetone; aqueous two-phase systems (ATPS) based on polyethylene glycol (PEG) and potassium phosphate; and direct immobilization. It was impossible to obtain stable precipitates of the enzyme due to the low concentration of total protein and the presence of biosurfactant produced by the microorganism. Different mixture compositions were selected for the partitioning study. Three ATPS showed selective partitioning of the target enzymes, i.e., lipase and protease migrated to opposite phases. In the ATPS composed of 13 wt% PEG-4000 and 10 wt% salts, it was possible to achieve a purification factor for lipase of 4.2. Purification by immobilization performed by lipase-lipase interactions showed three lipases of distinct sizes in the crude extract. In the immobilization method by hydrophobic supports, phenyl-agarose and butyl‑agarose were more selective in immobilizing than octyl-agarose. In the ion exchange immobilization method, only the lipases identified at 66 kDa and 41 kDa have an attraction for DEAE-agarose (anionic) and sulfopropyl-agarose (cationic) matrices. |
Palavras-Chave: |
Aqueous biphasic system; Aqueous two-phase system; Sistema aquoso bifásico; Sistema aquoso em duas fases. |
Thesaurus Nal: |
Chemical precipitation; Purification methods; Yarrowia lipolytica. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160760/1/ART-23038.pdf
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Marc: |
LEADER 02400naa a2200301 a 4500 001 2160760 005 2024-01-12 008 2023 bl uuuu u00u1 u #d 024 7 $a10.1016/j.clce.2023.100105$2DOI 100 1 $aBRIGIDA, A. I. S. 245 $aPartial purification of crude lipase extract from Yarrowia lipolytica$bprecipitation, aqueous two-phase systems (ATPS), and immobilization methods.$h[electronic resource] 260 $c2023 520 $aEfforts have been concentrated on developing alternative methods of enzyme purification that are less costly and highly efficient. In this work, we evaluated three different methods for lipase purification from Yarrowia lipolytica, such as precipitation using ammonium sulfate, ethanol, or acetone; aqueous two-phase systems (ATPS) based on polyethylene glycol (PEG) and potassium phosphate; and direct immobilization. It was impossible to obtain stable precipitates of the enzyme due to the low concentration of total protein and the presence of biosurfactant produced by the microorganism. Different mixture compositions were selected for the partitioning study. Three ATPS showed selective partitioning of the target enzymes, i.e., lipase and protease migrated to opposite phases. In the ATPS composed of 13 wt% PEG-4000 and 10 wt% salts, it was possible to achieve a purification factor for lipase of 4.2. Purification by immobilization performed by lipase-lipase interactions showed three lipases of distinct sizes in the crude extract. In the immobilization method by hydrophobic supports, phenyl-agarose and butyl‑agarose were more selective in immobilizing than octyl-agarose. In the ion exchange immobilization method, only the lipases identified at 66 kDa and 41 kDa have an attraction for DEAE-agarose (anionic) and sulfopropyl-agarose (cationic) matrices. 650 $aChemical precipitation 650 $aPurification methods 650 $aYarrowia lipolytica 653 $aAqueous biphasic system 653 $aAqueous two-phase system 653 $aSistema aquoso bifásico 653 $aSistema aquoso em duas fases 700 1 $aBUARQUE, F. S. 700 1 $aNOGUEIRA, V. L. R. 700 1 $aMELO, V. M. M. 700 1 $aGUISÁN SEIJAS, J. M. 700 1 $aRIBEIRO, B. D. 700 1 $aGONÇALVES, L. R. B. 700 1 $aCOELHO, M. A. Z. 773 $tCleaner Chemical Engineering$gv. 6, 11 p., art. 100105, June 2023.
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
09/10/1995 |
Data da última atualização: |
27/10/2005 |
Autoria: |
COSTA, N. P. da; FRANCA NETO, J. de B. |
Afiliação: |
EMBRAPA-CNPSo. Londrina, PR. |
Título: |
Avaliacao da qualidade da semente fiscalizada de soja produzida no Estado do Parana, na safra 1979/80. |
Ano de publicação: |
1980 |
Fonte/Imprenta: |
In: EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). Resultados de pesquisa de soja 1979/80. Londrina, 1980. |
Páginas: |
p.48-49. |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Parana; Quality; Seed; Soybean. |
Thesagro: |
Qualidade; Semente; Soja. |
Thesaurus NAL: |
Brazil. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00703naa a2200241 a 4500 001 1452129 005 2005-10-27 008 1980 bl uuuu u00u1 u #d 100 1 $aCOSTA, N. P. da 245 $aAvaliacao da qualidade da semente fiscalizada de soja produzida no Estado do Parana, na safra 1979/80. 260 $c1980 300 $ap.48-49. 650 $aBrazil 650 $aQualidade 650 $aSemente 650 $aSoja 653 $aBrasil 653 $aParana 653 $aQuality 653 $aSeed 653 $aSoybean 700 1 $aFRANCA NETO, J. de B. 773 $tIn: EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). Resultados de pesquisa de soja 1979/80. Londrina, 1980.
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Embrapa Soja (CNPSO) |
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