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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
23/02/2017 |
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Data da última atualização: |
29/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, V.; MASCARIN, G. M.; LOPES, M. da S.; ALVES, M. C. D. F.; REZENDE, J. M.; GATTI, M. S. V.; DUNLAP, C. A.; DELALIBERA JÚNIOR, I. |
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Afiliação: |
VIVIANE SANTOS, ESALQ; GABRIEL MOURA MASCARIN, CNPAF; MARIANA DA SILVA LOPES, ESALQ; MARIA CLARA DUARTE FREGOLENTE ALVES, UNICAMP; JANAYNE MARIA REZENDE, ESALQ; MARIA SILVIA VICCARI GATTI, UNICAMP; CHRISTOPHER A. DUNLAP, USDA; ITALO DELALIBERA JÚNIOR, ESALQ. |
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Título: |
Identification of double-stranded RNA viruses in Brazilian strains of Metarhizium anisopliae and their effects on fungal biology and virulence. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plant Gene, v. 11, p. 49-58, 2017. |
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ISSN: |
2352-4073 |
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DOI: |
10.1016/j.plgene.2017.01.001 |
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Idioma: |
Inglês |
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Conteúdo: |
Brazil is the leading country in the use of the entomopathogenic fungusMetarhiziumanisopliae against agricultural pests. Very little information has been documented about the diversity and the impact of mycoviruses on M. anisopliae compared to M. robertsii and M. brunneum. This study reports the discovery ofmycoviruses associated with native BrazilianMetarhiziumspp. strains fromthe entomopathogen collection of theUniversity of São Paulo, including strains used formass-production of aerial conidia in sugar-alcohol factories tomeet their own demand for controlling sugarcane spittlebugs and used in commercialmycoinsecticides.We also investigated the potential effects of viral infection on the biological traits of some of these strains. Viral-like double-stranded RNA (dsRNA) was found in 61% of the 36 Metarhizium spp. strains analyzed and showed a large variation of different electrophoretic banding patterns consisting of 2 to 8 dsRNA bands in polyacrylamide gels (PAGE). No dsRNA was detected in any of the strains from commercial products used in this study. Efforts to ?cure? strains with dsRNA revealed that the inhibitor of protein synthesis cycloheximidewas not efficient in eradicating dsRNA viruses from Metarhizium spp. strains. Some dsRNA segments of M. anisopliae ESALQ 26 were lost following hyphal tip subculturing. The dsRNA-variants of M. anisopliae strains ESALQ 866, ESALQ 1256 and ESALQ 1595 were healed after eithermonoconidial or hyphal tip subculturing. Single spore cultures of M. anisopliae ESALQ 1256 exhibited significant differences in vegetative growth, conidia production, and virulence against Tenebrio molitor larvae, although these differences were not linked with dsRNA. Moreover, fungal variants free of or positive for dsRNA had similar heat and UV tolerance responses. Our results suggest that viral-like dsRNA are very common among Brazilian M. anisopliae strains, but there is sparse evidence that these mycoviruses are harmful to fungal biological traits. MenosBrazil is the leading country in the use of the entomopathogenic fungusMetarhiziumanisopliae against agricultural pests. Very little information has been documented about the diversity and the impact of mycoviruses on M. anisopliae compared to M. robertsii and M. brunneum. This study reports the discovery ofmycoviruses associated with native BrazilianMetarhiziumspp. strains fromthe entomopathogen collection of theUniversity of São Paulo, including strains used formass-production of aerial conidia in sugar-alcohol factories tomeet their own demand for controlling sugarcane spittlebugs and used in commercialmycoinsecticides.We also investigated the potential effects of viral infection on the biological traits of some of these strains. Viral-like double-stranded RNA (dsRNA) was found in 61% of the 36 Metarhizium spp. strains analyzed and showed a large variation of different electrophoretic banding patterns consisting of 2 to 8 dsRNA bands in polyacrylamide gels (PAGE). No dsRNA was detected in any of the strains from commercial products used in this study. Efforts to ?cure? strains with dsRNA revealed that the inhibitor of protein synthesis cycloheximidewas not efficient in eradicating dsRNA viruses from Metarhizium spp. strains. Some dsRNA segments of M. anisopliae ESALQ 26 were lost following hyphal tip subculturing. The dsRNA-variants of M. anisopliae strains ESALQ 866, ESALQ 1256 and ESALQ 1595 were healed after eithermonoconidial or hyphal tip subculturing. Single spore c... Mostrar Tudo |
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Palavras-Chave: |
Fungal biology; Hyphal tip subculture; Monoconidial subculture; Mycovirus. |
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Thesagro: |
Metarhizium anisopliae. |
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Thesaurus Nal: |
Cycloheximide; DsRNA viruses; Stress tolerance; Virulence. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 03025naa a2200337 a 4500
001 2065515
005 2017-09-29
008 2017 bl uuuu u00u1 u #d
022 $a2352-4073
024 7 $a10.1016/j.plgene.2017.01.001$2DOI
100 1 $aSANTOS, V.
245 $aIdentification of double-stranded RNA viruses in Brazilian strains of Metarhizium anisopliae and their effects on fungal biology and virulence.$h[electronic resource]
260 $c2017
520 $aBrazil is the leading country in the use of the entomopathogenic fungusMetarhiziumanisopliae against agricultural pests. Very little information has been documented about the diversity and the impact of mycoviruses on M. anisopliae compared to M. robertsii and M. brunneum. This study reports the discovery ofmycoviruses associated with native BrazilianMetarhiziumspp. strains fromthe entomopathogen collection of theUniversity of São Paulo, including strains used formass-production of aerial conidia in sugar-alcohol factories tomeet their own demand for controlling sugarcane spittlebugs and used in commercialmycoinsecticides.We also investigated the potential effects of viral infection on the biological traits of some of these strains. Viral-like double-stranded RNA (dsRNA) was found in 61% of the 36 Metarhizium spp. strains analyzed and showed a large variation of different electrophoretic banding patterns consisting of 2 to 8 dsRNA bands in polyacrylamide gels (PAGE). No dsRNA was detected in any of the strains from commercial products used in this study. Efforts to ?cure? strains with dsRNA revealed that the inhibitor of protein synthesis cycloheximidewas not efficient in eradicating dsRNA viruses from Metarhizium spp. strains. Some dsRNA segments of M. anisopliae ESALQ 26 were lost following hyphal tip subculturing. The dsRNA-variants of M. anisopliae strains ESALQ 866, ESALQ 1256 and ESALQ 1595 were healed after eithermonoconidial or hyphal tip subculturing. Single spore cultures of M. anisopliae ESALQ 1256 exhibited significant differences in vegetative growth, conidia production, and virulence against Tenebrio molitor larvae, although these differences were not linked with dsRNA. Moreover, fungal variants free of or positive for dsRNA had similar heat and UV tolerance responses. Our results suggest that viral-like dsRNA are very common among Brazilian M. anisopliae strains, but there is sparse evidence that these mycoviruses are harmful to fungal biological traits.
650 $aCycloheximide
650 $aDsRNA viruses
650 $aStress tolerance
650 $aVirulence
650 $aMetarhizium anisopliae
653 $aFungal biology
653 $aHyphal tip subculture
653 $aMonoconidial subculture
653 $aMycovirus
700 1 $aMASCARIN, G. M.
700 1 $aLOPES, M. da S.
700 1 $aALVES, M. C. D. F.
700 1 $aREZENDE, J. M.
700 1 $aGATTI, M. S. V.
700 1 $aDUNLAP, C. A.
700 1 $aDELALIBERA JÚNIOR, I.
773 $tPlant Gene$gv. 11, p. 49-58, 2017.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/02/2017 |
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Data da última atualização: |
19/06/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
TEIXEIRA, R. N.; LIGTERINK, W.; FRANÇA-NETO, J. de B.; HILHORST, H. W. M.; SILVA, E. A. A. da. |
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Afiliação: |
RENAKE N. TEIXEIRA, UNESP Botucatu - Wageningen University; WILCO LIGTERINK, Wageningen University; JOSE DE BARROS FRANCA NETO, CNPSO; HENK W. M. HILHORST, Wageningen University; EDVALDO A. A. da SILVA, UNESP Botucatu. |
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Título: |
Gene expression profiling of the green seed problem in soybean. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
BMC Plant Biology, v. 16, n. 37, 2016. |
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Páginas: |
15 p. |
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ISSN: |
1471-2229 |
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DOI: |
10.1186/s12870-016-0729-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Due to the climate change of the past few decades, some agricultural areas in the world are now experiencing new climatic extremes. For soybean, high temperatures and drought stress can potentially lead to the ?green seed problem ? , which is characterized by chlorophyll retention in mature seeds and is associated with lower oil and seed quality, thus negatively impacting the production of soybean seeds. Here we show that heat and drought stress result in a ?mild ?stay-green phenotype and impaired expression of the STAY-GREEN 1 and STAY-GREEN 2 (D1, D2), PHEOPHORBIDASE 2 (PPH2) and NON-YELLOW COLORING 1 (NYC1_1) genes in soybean seeds of a susceptible soybean cultivar. We suggest that the higher expression of these genes in fully mature seeds of a tolerant cultivar allows these seeds to cope with stressful conditions and complete chlorophyll degradation. The gene expression results obtained in this study represent a significant advance in understanding chlorophyll retention in mature soybean seeds produced under stressful conditions. This will open new research possibilities towards finding molecular markers for breeding programs to produce cultivars which are less susceptible to chlorophyll retention under the hot and dry climate conditions which are increasingly common in the largest soybean production areas of the world. |
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Thesagro: |
Qualidade; Semente; Soja. |
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Thesaurus NAL: |
Seed quality; Soybeans. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154844/1/Gene-expression....pdf
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Marc: |
LEADER 02054naa a2200265 a 4500
001 2062882
005 2017-06-19
008 2016 bl uuuu u00u1 u #d
022 $a1471-2229
024 7 $a10.1186/s12870-016-0729-0$2DOI
100 1 $aTEIXEIRA, R. N.
245 $aGene expression profiling of the green seed problem in soybean.$h[electronic resource]
260 $c2016
300 $a15 p.
520 $aDue to the climate change of the past few decades, some agricultural areas in the world are now experiencing new climatic extremes. For soybean, high temperatures and drought stress can potentially lead to the ?green seed problem ? , which is characterized by chlorophyll retention in mature seeds and is associated with lower oil and seed quality, thus negatively impacting the production of soybean seeds. Here we show that heat and drought stress result in a ?mild ?stay-green phenotype and impaired expression of the STAY-GREEN 1 and STAY-GREEN 2 (D1, D2), PHEOPHORBIDASE 2 (PPH2) and NON-YELLOW COLORING 1 (NYC1_1) genes in soybean seeds of a susceptible soybean cultivar. We suggest that the higher expression of these genes in fully mature seeds of a tolerant cultivar allows these seeds to cope with stressful conditions and complete chlorophyll degradation. The gene expression results obtained in this study represent a significant advance in understanding chlorophyll retention in mature soybean seeds produced under stressful conditions. This will open new research possibilities towards finding molecular markers for breeding programs to produce cultivars which are less susceptible to chlorophyll retention under the hot and dry climate conditions which are increasingly common in the largest soybean production areas of the world.
650 $aSeed quality
650 $aSoybeans
650 $aQualidade
650 $aSemente
650 $aSoja
700 1 $aLIGTERINK, W.
700 1 $aFRANÇA-NETO, J. de B.
700 1 $aHILHORST, H. W. M.
700 1 $aSILVA, E. A. A. da
773 $tBMC Plant Biology$gv. 16, n. 37, 2016.
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