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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Soja. |
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Data corrente: |
08/12/2022 |
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Data da última atualização: |
08/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DUARTE, K E. D.; BASSO, M. F.; OLIVEIRA, N. G. de; SILVA, J. C. F. da; GARCIA, B. de O.; DIAS, B. B. A.; CARDOSO, T. B.; NEPOMUCENO, A. L.; KOBAYASHI, A. K.; SANTIAGO, T. R.; SOUZA, W. R. de; MOLINARI, H. B. C. |
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Afiliação: |
KAROLINE ESTEFANI DUARTE, Federal University of ABC; MARCOS FERNANDO BASSO, Mato Grosso Cotton Institute; NELSON GERALDO DE OLIVEIRA, EMBRAPA AGROENERGY; JOSÉ CLEYDSON FERREIRA DA SILVA, FEDERAL UNIVERSITY OF VIÇOSA; BRUNO DE OLIVEIRA GARCIA, Federal University of Viçosa; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; THIAGO BERGAMO CARDOSO, Sempre AGTECH; ALEXANDRE LIMA NEPOMUCENO, CNPSO; ADILSON KENJI KOBAYASHI, CNPAE; THAÍS RIBEIRO SANTIAGO, UNIVERSITY OF BRASÍLIA; WAGNER RODRIGO DE SOUZA, Federal University of ABC; HUGO BRUNO CORREA MOLINARI, Sempre AGTECH. |
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Título: |
MicroRNAs expression profiles in early responses to different levels of water deficit in Setaria viridis. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Physiology and Molecular Biology of Plants, v. 28, n. 8, p. 1607-1624, 2022. |
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DOI: |
https://doi.org/10.1007/s12298-022-01226-z |
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Idioma: |
Inglês |
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Conteúdo: |
Water deficit is a major constraint for crops of economic importance in almost all agricultural regions. However, plants have an active defense system to adapt to these adverse conditions, acting in the reprogramming of gene expression responsible for encoding microRNAs (miRNAs). These miRNAs promote the regulation to the target gene expression by the post-transcriptional (PTGS) and transcriptional gene silencing (TGS), modulating several pathways including defense response to water deficit. The broader knowledge of the miRNA expression profile and its regulatory networks in response to water deficit can provide evidence for the development of new biotechnological tools for genetic improvement of several important crops. In this study, we used Setaria viridis accession A10.1 as a C4 model plant to widely investigate the miRNA expression profile in early responses to different levels of water deficit. Ecophysiological studies in Setaria viridis under water deficit and after rewatering demonstrated a drought tolerant accession, capable of a rapid recovery from the stress. Deep small RNA sequencing and degradome studies were performed in plants submitted to drought to identify differentially expressed miRNA genes and their predicted targets, using in silico analysis. Our findings showed that several miRNAs were differentially modulated in response to distinctive levels of water deficit and after rewatering. The predicted mRNA targets mainly corresponded to genes related to cell wall remodeling, antioxidant system and drought-related transcription factors, indicating that these genes are rapidly regulated in early responses to drought stress. The implications of these modulations are extensively discussed, and higher-effect miRNAs are suggested as major players for potential use in genetic engineering to improve drought tolerance in economically important crops, such as sugarcane, maize, and sorghum. MenosWater deficit is a major constraint for crops of economic importance in almost all agricultural regions. However, plants have an active defense system to adapt to these adverse conditions, acting in the reprogramming of gene expression responsible for encoding microRNAs (miRNAs). These miRNAs promote the regulation to the target gene expression by the post-transcriptional (PTGS) and transcriptional gene silencing (TGS), modulating several pathways including defense response to water deficit. The broader knowledge of the miRNA expression profile and its regulatory networks in response to water deficit can provide evidence for the development of new biotechnological tools for genetic improvement of several important crops. In this study, we used Setaria viridis accession A10.1 as a C4 model plant to widely investigate the miRNA expression profile in early responses to different levels of water deficit. Ecophysiological studies in Setaria viridis under water deficit and after rewatering demonstrated a drought tolerant accession, capable of a rapid recovery from the stress. Deep small RNA sequencing and degradome studies were performed in plants submitted to drought to identify differentially expressed miRNA genes and their predicted targets, using in silico analysis. Our findings showed that several miRNAs were differentially modulated in response to distinctive levels of water deficit and after rewatering. The predicted mRNA targets mainly corresponded to genes related to cell... Mostrar Tudo |
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Thesagro: |
Bioenergia; Setária. |
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Thesaurus Nal: |
Abiotic stress; Bioenergy; Drought; Setaria viridis. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1149404/1/MicroRNAs.pdf
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Marc: |
LEADER 02948naa a2200337 a 4500
001 2149404
005 2022-12-08
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s12298-022-01226-z$2DOI
100 1 $aDUARTE, K E. D.
245 $aMicroRNAs expression profiles in early responses to different levels of water deficit in Setaria viridis.$h[electronic resource]
260 $c2022
520 $aWater deficit is a major constraint for crops of economic importance in almost all agricultural regions. However, plants have an active defense system to adapt to these adverse conditions, acting in the reprogramming of gene expression responsible for encoding microRNAs (miRNAs). These miRNAs promote the regulation to the target gene expression by the post-transcriptional (PTGS) and transcriptional gene silencing (TGS), modulating several pathways including defense response to water deficit. The broader knowledge of the miRNA expression profile and its regulatory networks in response to water deficit can provide evidence for the development of new biotechnological tools for genetic improvement of several important crops. In this study, we used Setaria viridis accession A10.1 as a C4 model plant to widely investigate the miRNA expression profile in early responses to different levels of water deficit. Ecophysiological studies in Setaria viridis under water deficit and after rewatering demonstrated a drought tolerant accession, capable of a rapid recovery from the stress. Deep small RNA sequencing and degradome studies were performed in plants submitted to drought to identify differentially expressed miRNA genes and their predicted targets, using in silico analysis. Our findings showed that several miRNAs were differentially modulated in response to distinctive levels of water deficit and after rewatering. The predicted mRNA targets mainly corresponded to genes related to cell wall remodeling, antioxidant system and drought-related transcription factors, indicating that these genes are rapidly regulated in early responses to drought stress. The implications of these modulations are extensively discussed, and higher-effect miRNAs are suggested as major players for potential use in genetic engineering to improve drought tolerance in economically important crops, such as sugarcane, maize, and sorghum.
650 $aAbiotic stress
650 $aBioenergy
650 $aDrought
650 $aSetaria viridis
650 $aBioenergia
650 $aSetária
700 1 $aBASSO, M. F.
700 1 $aOLIVEIRA, N. G. de
700 1 $aSILVA, J. C. F. da
700 1 $aGARCIA, B. de O.
700 1 $aDIAS, B. B. A.
700 1 $aCARDOSO, T. B.
700 1 $aNEPOMUCENO, A. L.
700 1 $aKOBAYASHI, A. K.
700 1 $aSANTIAGO, T. R.
700 1 $aSOUZA, W. R. de
700 1 $aMOLINARI, H. B. C.
773 $tPhysiology and Molecular Biology of Plants$gv. 28, n. 8, p. 1607-1624, 2022.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças; Embrapa Instrumentação. |
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Data corrente: |
12/08/2022 |
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Data da última atualização: |
12/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
MIRANDA, M.; RIBEIRO, M. de M. M.; SPRICIGO, P. C.; PILON, L.; FOSCHINI, M. M.; CORREA, D. S.; FERREIRA, M. D. |
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Afiliação: |
MARCELA MIRANDA, UNIVERSIDADE FEDERAL DE SÃO CARLOS; MARILENE DE MORI M. RIBEIRO, QGP Tanquímica; POLIANA C. SPRICIGO, USP/ESALQ; LUCIMEIRE PILON, CNPH; MILENE CORSO MITSUYUKI, CNPDIA; DANIEL SOUZA CORREA, CNPDIA; MARCOS DAVID FERREIRA, CNPDIA. |
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Título: |
Carnauba wax nanoemulsion applied as an edible coating on fresh tomato for postharvest quality evaluation. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Heliyon, v. 8, e09803, 2022. |
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ISSN: |
2405-8440 |
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DOI: |
https://doi.org/10.1016/j.heliyon.2022.e09803 |
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Idioma: |
Inglês |
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Conteúdo: |
Edible coatings to extend the shelf life and preserve the quality of fruit and vegetables are highly demanded nowadays. Recently, plant-based edible coatings have gained importance in the context of sustainability, which in combination with suitable top-down process can render ?greener? nanoemulsions with optimized properties. Herein we developed a carnauba wax nanoemulsion (CWN) by using a high-pressure processing to be applied as an edible coating for fruit and vegetables. The as-developed nanoemulsion properties were compared to conventional carnauba wax emulsion (CWM), where CWN showed particle size diameter of 44 nm and narrow distribution, while CWM displayed larger particles and wider size distribution (from 200 to 1700 nm). For assessment of the postharvest quality, cv. ?Debora? tomatoes, employed here as a model, were coated with CWN or CWM, at concentrations of 9 and 18%, and then compared to uncoated fruit during storage at 23 ºC for 15 days. Evaluation of fruit quality, including sugar, acids, pH, water vapor loss, firmness, gloss, color, ethylene and respiratory activity, were assessed at every 3 days, while sensory test were carried out at the end of storage. Uncoated tomatoes presented the highest water loss values, meanwhile, firmness, ethylene, and respiratory activity were not largely modified by the coatings during storage. Tomatoes coated with the CWN exhibited the highest instrumental gloss and were preferred by consumers in sensory evaluations, indicating the potential of the as-developed carnauba wax green nanoemulsion for postharvest applications. MenosEdible coatings to extend the shelf life and preserve the quality of fruit and vegetables are highly demanded nowadays. Recently, plant-based edible coatings have gained importance in the context of sustainability, which in combination with suitable top-down process can render ?greener? nanoemulsions with optimized properties. Herein we developed a carnauba wax nanoemulsion (CWN) by using a high-pressure processing to be applied as an edible coating for fruit and vegetables. The as-developed nanoemulsion properties were compared to conventional carnauba wax emulsion (CWM), where CWN showed particle size diameter of 44 nm and narrow distribution, while CWM displayed larger particles and wider size distribution (from 200 to 1700 nm). For assessment of the postharvest quality, cv. ?Debora? tomatoes, employed here as a model, were coated with CWN or CWM, at concentrations of 9 and 18%, and then compared to uncoated fruit during storage at 23 ºC for 15 days. Evaluation of fruit quality, including sugar, acids, pH, water vapor loss, firmness, gloss, color, ethylene and respiratory activity, were assessed at every 3 days, while sensory test were carried out at the end of storage. Uncoated tomatoes presented the highest water loss values, meanwhile, firmness, ethylene, and respiratory activity were not largely modified by the coatings during storage. Tomatoes coated with the CWN exhibited the highest instrumental gloss and were preferred by consumers in sensory evaluations, indicati... Mostrar Tudo |
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Palavras-Chave: |
Cera de carnaúba; Copernicia prunifera; Emerging processing technologies; GRAS-ingredients; Sustainable chemistry. |
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Thesagro: |
Armazenamento de Alimento; Embalagem; Tomate. |
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Thesaurus NAL: |
Carnauba wax; Nanomaterials. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1145407/1/Miranda-et-al-Heliyon-v-8-e09803-2022.pdf
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Marc: |
LEADER 02600naa a2200337 a 4500
001 2145407
005 2022-08-12
008 2022 bl uuuu u00u1 u #d
022 $a2405-8440
024 7 $ahttps://doi.org/10.1016/j.heliyon.2022.e09803$2DOI
100 1 $aMIRANDA, M.
245 $aCarnauba wax nanoemulsion applied as an edible coating on fresh tomato for postharvest quality evaluation.$h[electronic resource]
260 $c2022
520 $aEdible coatings to extend the shelf life and preserve the quality of fruit and vegetables are highly demanded nowadays. Recently, plant-based edible coatings have gained importance in the context of sustainability, which in combination with suitable top-down process can render ?greener? nanoemulsions with optimized properties. Herein we developed a carnauba wax nanoemulsion (CWN) by using a high-pressure processing to be applied as an edible coating for fruit and vegetables. The as-developed nanoemulsion properties were compared to conventional carnauba wax emulsion (CWM), where CWN showed particle size diameter of 44 nm and narrow distribution, while CWM displayed larger particles and wider size distribution (from 200 to 1700 nm). For assessment of the postharvest quality, cv. ?Debora? tomatoes, employed here as a model, were coated with CWN or CWM, at concentrations of 9 and 18%, and then compared to uncoated fruit during storage at 23 ºC for 15 days. Evaluation of fruit quality, including sugar, acids, pH, water vapor loss, firmness, gloss, color, ethylene and respiratory activity, were assessed at every 3 days, while sensory test were carried out at the end of storage. Uncoated tomatoes presented the highest water loss values, meanwhile, firmness, ethylene, and respiratory activity were not largely modified by the coatings during storage. Tomatoes coated with the CWN exhibited the highest instrumental gloss and were preferred by consumers in sensory evaluations, indicating the potential of the as-developed carnauba wax green nanoemulsion for postharvest applications.
650 $aCarnauba wax
650 $aNanomaterials
650 $aArmazenamento de Alimento
650 $aEmbalagem
650 $aTomate
653 $aCera de carnaúba
653 $aCopernicia prunifera
653 $aEmerging processing technologies
653 $aGRAS-ingredients
653 $aSustainable chemistry
700 1 $aRIBEIRO, M. de M. M.
700 1 $aSPRICIGO, P. C.
700 1 $aPILON, L.
700 1 $aFOSCHINI, M. M.
700 1 $aCORREA, D. S.
700 1 $aFERREIRA, M. D.
773 $tHeliyon$gv. 8, e09803, 2022.
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Embrapa Hortaliças (CNPH) |
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