Data/hora: 10/06/2024 14:06:49

Biblioteca(s): | Embrapa Instrumentação.
Data corrente: | 19/11/2004
Data da última atualização: | 08/03/2010
Autoria: | BUGS, M. R.; FORATO, L. A.; BORTOLETO-BUGS, R. K.; FISCHER, H.; MASCARENHAS, Y. P.; WARD, R. J.; COLNAGO, L. A.
Título: | Spectroscopy characterization and structural modeling of prolamin from maize and pearl millet.
Ano de publicação: | 2004
Fonte/Imprenta: | European Biophysics Journal, New York, v. 33, p. 335-343, 2004.
Idioma: | Inglês
Conteúdo: | Biophysical methods and structural modeling techniques have been used to characterize the prolamins from maize (Zea mays) and pearl millet (Pennisetum americanum). The alcohol-soluble prolamin from maize, called zein, was extracted using a simple protocol and purified by gel filtration in a 70% ethanol solution. Two protein fractions were purified from seed extracts of pearl millet with molecular weights of 25.5 and 7 kDa, as estimated by SDS-PAGE. The high molecular weight protein corresponds to pennisetin, wich has a high helical content both in solution and the solid state, as demonstrated by circular dichroism and Fourier transform infrared spectra.
Palavras-Chave: | Dicroísmo circular; Dinâmico; Modelo molecular.
Thesagro: | Zeína.
Categoria do assunto: | --
Registro original: | Embrapa Instrumentação (CNPDIA)
Exemplares: | CNPDIA - (8473-1) - UPC - SP - -- - PROCI-04.00027 -  - 2004.00027 - DIS