| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
25/09/2007 |
|
Data da última atualização: |
09/08/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BARBOSA, S.; DAVIDE, L. C.; PEREIRA, A. V.; ABREU, J. C. de. |
|
Afiliação: |
Sandro Barbosa Universidade Federal de Alfenas - UNIFAL MG; Lisete Chamma Davide, Universidade Federal de Lavras; Antônio Vander Pereira, Embrapa Gado de Leite; Juscélio Clemente de Abreu Universidade Vale do Rio Verde - UninCor Três Corações MG. |
|
Título: |
Duplicação cromossômica de híbridos triplóides de capim-elefante e milheto. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
Bragantia, Campinas, v. 66, n. 3, p. 365-372, 2007. |
|
DOI: |
https://doi.org/10.1590/S0006-87052007000300001 |
|
Idioma: |
Português |
|
Conteúdo: |
RESUMO - O objetivo deste estudo foi duplicar o complemento cromossômico de híbridos triplóides de capimelefante e milheto para obtenção de plantas hexaplóides férteis. Esta estratégia permitirá o uso de hexaplóides em programas de melhoramento genético do capim-elefante, por meio da transferência de alelos com as características desejadas, tornando possível sua propagação via semente. Seedlings, plântulas e segmentos caulinares cultivados in vitro foram tratados com colchicina a 0,05% ou 0,1% aplicadas por 12 ou 24 horas. Seedlings de genótipos híbridos diferentes e meristemas de dois híbridos interespecíficos cultivados in vivo foram tratados por 24 horas com colchicina a 0,05%. A duplicação cromossômica foi confirmada pela contagem de cromossomos em células meristemáticas de pontas de raízes. A viabilidade polínica, os testes de germinação in vitro e a produção de sementes foram utilizados para avaliar a fertilidade dos hexaplóides. Seedlings cultivados in vitro e tratados com colchicina a 0,1% por 24 horas tiveram a melhor resposta à indução de poliploidia; em 38% das plantas sobreviventes observaram-se o conjunto cromossômico duplicado e a presença de pólens viáveis, confirmando a fertilidade dos hexaplóides. ABSTRACT - The aim of this study was to duble the chromose number of elephantgrass and pearl millet triploid hybrids in order to obtain hexaploid fertile plants. This strategy would allow the use of the hexaploids in the elephantgrass breeding program as a bridge for transfering alleles of desired characteristics and making possible propagation by seed. Seedlings, plantlets and stem segments were cultivated in vitro and treated with 0.05% or 0.1% colchicine for 12 or 24 h. Seedlings of different hybrid genotypes and meristems of two interspecific hybrids were cultivated in vivo and treated for 24 h with 0.05% colchicine. Chromose duplication was confirmed by countings in the root tip cells. Pollen viability, in vitro germination tests and seed production were used to evaluate the fertility of the hexaploids. Seedlings cultivated in vitro and treated with 0.1% colchicine for 24 h were the most efficient method of polyploidy induction, as 38% of the surviving plants have twice the number of chromose viable pollen grains and fertile seeds. MenosRESUMO - O objetivo deste estudo foi duplicar o complemento cromossômico de híbridos triplóides de capimelefante e milheto para obtenção de plantas hexaplóides férteis. Esta estratégia permitirá o uso de hexaplóides em programas de melhoramento genético do capim-elefante, por meio da transferência de alelos com as características desejadas, tornando possível sua propagação via semente. Seedlings, plântulas e segmentos caulinares cultivados in vitro foram tratados com colchicina a 0,05% ou 0,1% aplicadas por 12 ou 24 horas. Seedlings de genótipos híbridos diferentes e meristemas de dois híbridos interespecíficos cultivados in vivo foram tratados por 24 horas com colchicina a 0,05%. A duplicação cromossômica foi confirmada pela contagem de cromossomos em células meristemáticas de pontas de raízes. A viabilidade polínica, os testes de germinação in vitro e a produção de sementes foram utilizados para avaliar a fertilidade dos hexaplóides. Seedlings cultivados in vitro e tratados com colchicina a 0,1% por 24 horas tiveram a melhor resposta à indução de poliploidia; em 38% das plantas sobreviventes observaram-se o conjunto cromossômico duplicado e a presença de pólens viáveis, confirmando a fertilidade dos hexaplóides. ABSTRACT - The aim of this study was to duble the chromose number of elephantgrass and pearl millet triploid hybrids in order to obtain hexaploid fertile plants. This strategy would allow the use of the hexaploids in the elephantgrass breeding program as a bridge f... Mostrar Tudo |
|
Palavras-Chave: |
Duplicação cromossômica; híbrido interespecífico. |
|
Thesagro: |
Colchicina; Pennisetum Glaucum; Pennisetum Purpureum; Poliploidia. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/595808/1/Duplicacao-cromossomica-de-hibridos-triploides-de-capim-elefante-e-milheto.pdf
|
|
Marc: |
LEADER 03077naa a2200241 a 4500
001 1595808
005 2022-08-09
008 2007 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S0006-87052007000300001$2DOI
100 1 $aBARBOSA, S.
245 $aDuplicação cromossômica de híbridos triplóides de capim-elefante e milheto.$h[electronic resource]
260 $c2007
520 $aRESUMO - O objetivo deste estudo foi duplicar o complemento cromossômico de híbridos triplóides de capimelefante e milheto para obtenção de plantas hexaplóides férteis. Esta estratégia permitirá o uso de hexaplóides em programas de melhoramento genético do capim-elefante, por meio da transferência de alelos com as características desejadas, tornando possível sua propagação via semente. Seedlings, plântulas e segmentos caulinares cultivados in vitro foram tratados com colchicina a 0,05% ou 0,1% aplicadas por 12 ou 24 horas. Seedlings de genótipos híbridos diferentes e meristemas de dois híbridos interespecíficos cultivados in vivo foram tratados por 24 horas com colchicina a 0,05%. A duplicação cromossômica foi confirmada pela contagem de cromossomos em células meristemáticas de pontas de raízes. A viabilidade polínica, os testes de germinação in vitro e a produção de sementes foram utilizados para avaliar a fertilidade dos hexaplóides. Seedlings cultivados in vitro e tratados com colchicina a 0,1% por 24 horas tiveram a melhor resposta à indução de poliploidia; em 38% das plantas sobreviventes observaram-se o conjunto cromossômico duplicado e a presença de pólens viáveis, confirmando a fertilidade dos hexaplóides. ABSTRACT - The aim of this study was to duble the chromose number of elephantgrass and pearl millet triploid hybrids in order to obtain hexaploid fertile plants. This strategy would allow the use of the hexaploids in the elephantgrass breeding program as a bridge for transfering alleles of desired characteristics and making possible propagation by seed. Seedlings, plantlets and stem segments were cultivated in vitro and treated with 0.05% or 0.1% colchicine for 12 or 24 h. Seedlings of different hybrid genotypes and meristems of two interspecific hybrids were cultivated in vivo and treated for 24 h with 0.05% colchicine. Chromose duplication was confirmed by countings in the root tip cells. Pollen viability, in vitro germination tests and seed production were used to evaluate the fertility of the hexaploids. Seedlings cultivated in vitro and treated with 0.1% colchicine for 24 h were the most efficient method of polyploidy induction, as 38% of the surviving plants have twice the number of chromose viable pollen grains and fertile seeds.
650 $aColchicina
650 $aPennisetum Glaucum
650 $aPennisetum Purpureum
650 $aPoliploidia
653 $aDuplicação cromossômica
653 $ahíbrido interespecífico
700 1 $aDAVIDE, L. C.
700 1 $aPEREIRA, A. V.
700 1 $aABREU, J. C. de
773 $tBragantia, Campinas$gv. 66, n. 3, p. 365-372, 2007.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
22/11/2021 |
|
Data da última atualização: |
22/11/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
AMARAL, G. C.; PEZZOPANE, J. E. M.; NÓIA JÚNIOR, R. DE S.; FERNÁNDEZ MARTÍNEZ, M.; FONSECA, M. D. S.; GIBSON, E. L.; TOLEDO, J. V.; PEZZOPANE, J. R. M.; KLIPPEL, V. H.; XAVIER, T. M. T. |
|
Afiliação: |
GENILDA CANUTO AMARAL, UFES; JOSÉ EDUARDO MACEDO PEZZOPANE, UFES; ROGÉRIO DE SOUZA NÓIA JÚNIOR, University of Munich; MANUEL FERNÁNDEZ MARTÍNEZ, University of Huelva; MARIANA DUARTE SILVA FONSECA, UFES; ELBYA LEÃO GIBSON, UFES; JOÃO VITOR TOLEDO, UFES; JOSE RICARDO MACEDO PEZZOPANE, CPPSE; VALÉRIA HOLLUNDER KLIPPEL, UFES; TALITA MIRANDA TEIXEIRA XAVIER, UFES. |
|
Título: |
Pilocarpus microphyllus seedling growth threatened by climate change: an ecophysiological approach. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Theoretical and Applied Climatology, 2021. |
|
Páginas: |
15 p. |
|
DOI: |
https://doi.org/10.1007/s00704-021-03831-6 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The climate change endangers many species of the Amazon Forest. An example, in the endangered medicinal species Pilocarpus microphyllus (popularly known as jaborandi). The jaborandi is a natural source of alkaloids used for serious diseases, essential for medical use. However, the potential impacts of climate change on this species are still unknown. Thus, the objective was to evaluate the effect of high temperature and high atmospheric concentrations of CO2, projected to occur with climate change, combined with water stress, on the ecophysiology of jaborandi seedlings. For this, seedlings were exposed to three different climatic scenarios in a controlled environment, characterized as current Amazon: RCP4.5 (current Amazon average temperature +2.5ºC and 538 ppm of [CO2]) and RCP 8.5 (+4.5 °C and 936 ppm of [CO2]). Within each climatic scenario, two levels of irrigation were applied: 90% (well-watered) and 40% (water-stressed) of the substrate water holding capacity. Growth variables, plant water status, fluorescence parameters, and enzymatic antioxidants activity were evaluated. The results showed that the jaborandi seedlings showed greater total dry mass production when grown in the current Amazon with no water stress. The seedlings? growth was negatively affected by the scenarios enriched with CO2, especially RCP 8.5. Climate change scenarios had the greatest negative impact when the seedlings were subject to water stress. Under these scenarios of climate change and water stress, the seedlings allocated more carbohydrates to the root system. The negative effect of water stress on jaborandi physiology and growth was attenuated by the RCP 8.5 scenario. Our results indicated that the predicted climate changes negatively impact ecophysiology jaborandi, being a threat to the extinction of this species. MenosThe climate change endangers many species of the Amazon Forest. An example, in the endangered medicinal species Pilocarpus microphyllus (popularly known as jaborandi). The jaborandi is a natural source of alkaloids used for serious diseases, essential for medical use. However, the potential impacts of climate change on this species are still unknown. Thus, the objective was to evaluate the effect of high temperature and high atmospheric concentrations of CO2, projected to occur with climate change, combined with water stress, on the ecophysiology of jaborandi seedlings. For this, seedlings were exposed to three different climatic scenarios in a controlled environment, characterized as current Amazon: RCP4.5 (current Amazon average temperature +2.5ºC and 538 ppm of [CO2]) and RCP 8.5 (+4.5 °C and 936 ppm of [CO2]). Within each climatic scenario, two levels of irrigation were applied: 90% (well-watered) and 40% (water-stressed) of the substrate water holding capacity. Growth variables, plant water status, fluorescence parameters, and enzymatic antioxidants activity were evaluated. The results showed that the jaborandi seedlings showed greater total dry mass production when grown in the current Amazon with no water stress. The seedlings? growth was negatively affected by the scenarios enriched with CO2, especially RCP 8.5. Climate change scenarios had the greatest negative impact when the seedlings were subject to water stress. Under these scenarios of climate change and water ... Mostrar Tudo |
|
Palavras-Chave: |
Climatic scenario; Ecophysiology of jaborandi seedlings; High atmospheric concentrations of CO2. |
|
Thesagro: |
Jaborandi; Pilocarpus Microphyllus. |
|
Thesaurus NAL: |
Water stress. |
|
Categoria do assunto: |
A Sistemas de Cultivo |
|
Marc: |
LEADER 02854naa a2200325 a 4500
001 2136378
005 2021-11-22
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00704-021-03831-6$2DOI
100 1 $aAMARAL, G. C.
245 $aPilocarpus microphyllus seedling growth threatened by climate change$ban ecophysiological approach.$h[electronic resource]
260 $c2021
300 $a15 p.
520 $aThe climate change endangers many species of the Amazon Forest. An example, in the endangered medicinal species Pilocarpus microphyllus (popularly known as jaborandi). The jaborandi is a natural source of alkaloids used for serious diseases, essential for medical use. However, the potential impacts of climate change on this species are still unknown. Thus, the objective was to evaluate the effect of high temperature and high atmospheric concentrations of CO2, projected to occur with climate change, combined with water stress, on the ecophysiology of jaborandi seedlings. For this, seedlings were exposed to three different climatic scenarios in a controlled environment, characterized as current Amazon: RCP4.5 (current Amazon average temperature +2.5ºC and 538 ppm of [CO2]) and RCP 8.5 (+4.5 °C and 936 ppm of [CO2]). Within each climatic scenario, two levels of irrigation were applied: 90% (well-watered) and 40% (water-stressed) of the substrate water holding capacity. Growth variables, plant water status, fluorescence parameters, and enzymatic antioxidants activity were evaluated. The results showed that the jaborandi seedlings showed greater total dry mass production when grown in the current Amazon with no water stress. The seedlings? growth was negatively affected by the scenarios enriched with CO2, especially RCP 8.5. Climate change scenarios had the greatest negative impact when the seedlings were subject to water stress. Under these scenarios of climate change and water stress, the seedlings allocated more carbohydrates to the root system. The negative effect of water stress on jaborandi physiology and growth was attenuated by the RCP 8.5 scenario. Our results indicated that the predicted climate changes negatively impact ecophysiology jaborandi, being a threat to the extinction of this species.
650 $aWater stress
650 $aJaborandi
650 $aPilocarpus Microphyllus
653 $aClimatic scenario
653 $aEcophysiology of jaborandi seedlings
653 $aHigh atmospheric concentrations of CO2
700 1 $aPEZZOPANE, J. E. M.
700 1 $aNÓIA JÚNIOR, R. DE S.
700 1 $aFERNÁNDEZ MARTÍNEZ, M.
700 1 $aFONSECA, M. D. S.
700 1 $aGIBSON, E. L.
700 1 $aTOLEDO, J. V.
700 1 $aPEZZOPANE, J. R. M.
700 1 $aKLIPPEL, V. H.
700 1 $aXAVIER, T. M. T.
773 $tTheoretical and Applied Climatology, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
