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| Registros recuperados : 538 | |
| 101. |  | BALARO, M. F. A.; FONSECA, J. F. da; OBA, E.; CARDOSO, E. da C.; BRANDÃO, F. Z. Is the Santa Inês sheep a typical non-seasonal breeder in the Brazilian Southeast? Tropical Animal Health and Production, Edinburgh, v. 46, n. 8, p. 1533-1537, Dec. 2014.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 102. | | BALARO, M. F. A.; SANTOS, A. S.; MOURA, L. F. G. M.; FONSECA, J. F. da; BRANDÃO, F. Z. Luteal dynamic and functionality assessment in dairy goats by luteal blood flow, luteal biometry, and hormonal assay. Theriogenology, v. 95, p. 118-126, June, 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 103. | | FONSECA, J. F. da; SOUZA, J. M. G. de; VIEIRA, L. E.; BRUSCHI, J. H.; BRANDÃO, F. Z. Manejo reprodutivo de caprinos e ovinos na região Norte do Brasil. In: ZOOTEC NA AMAZÔNIA LEGAL, 1.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 20., 2010, Palmas. Sustentabilidade e produção animal: anais das palestras. Araguaiana: Universidade Federal de Tocantins: Associação Brasileira de Zootecnistas, 2010. p. 177-197.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 105. | | ZAMBRINI, F. N.; COSTA, E. P.; BRUSCHI, J. H.; SOUZA, J. M. G.; AMORIM, L. S.; FONSECA, J. F. da. Effects of hormonal administration in two different shifts and ovulatory dynamics in nonbreeding season on Toggenburg goats. Reproduction, Fertility and Development, v. 22, n. 1, p. 381, 2010. Edição dos Proceedings of the Annual Conference of the International Embryo Transfer Society, Córdoba, Argentina, 9?12 January 2010.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 106. | | ZAMBRINI, F. N.; COSTA, E. P.; BRUSCHI, J. H.; SOUZA, J. M. G.; AMORIM, L. S.; FONSECA, J. F. da. Effects of hormonal administration in two different in two different shifts and ovulatory dynamics in nonbreeding season on toggenburg goats. Reproduction, Fertility and Development, v. 22, n. 1, 2010. Edição dos proceedings do Annual Conference of the International Embryo, 2010, Cordoba.| Biblioteca(s): Embrapa Gado de Leite. |
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| 107. | | NASCIMENTO-PENIDO, P. M. P.; BRANDÃO, F. C.; PENIDO, A. O.; PEREIRA, P. F. V.; FONSECA, J. F. da. Parâmetros reprodutivos após indução de estro com diferentes intervalos de permanência do dispositivo vaginal em cabras Toggenburg acíclicas. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 70, n. 3, p. 773-778, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 108. | | VIANA, J. H. M.; PALHÃO, M. P.; SIQUEIRA, L. G. B.; FONSECA, J. F. da; CAMARGO, L. S. de A. Ovarian follicular dynamics, follicle deviation, and oocyte yield in Gyr breed (Bos indicus) cows undergoing repeated ovum pick-up. Theriogenology, v. 73, n. 7, p. 966-972, 2010.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 111. | | PINNA, A. E.; BRANDÃO, F. Z.; CAVALCANTI, A. S.; BORGES, A. M.; SOUZA, J. M. G.; FONSECA, J. F. da. Reproductive parameters of Santa Inês ewes submitted to short-term treatment with re-used progesterone devices. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 64, n. 2, p. 333-340, 2012.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 112. | | NASCIMENTO-PENIDO, P. M. P. do; OLIVEIRA, A. P.; GALINARI, G. C. F.; FONSECA, J. F. da; LEITE, R. C. Risk of transmission of caprine arthritis - encephalitis virus (CAEV) through embryos transfer from naturally infected donors. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 503, jan./mar. 2017. Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 114. | | MARVULLE, V.; MACHADO, V. de O.; BONATO, G. C.; ARRAIS, A. M.; PUGLIESI, G.; FONSECA, J. F. da. Uso de diferentes doses de sêmen congelado/descongelado e fertilidade em cabras leiteiras na contra-estação: resultados preliminares. Revista Brasileira de Reprodução Animal, v. 43, n. 2, p. 551, abr./jun. 2019. Edição dos resumos do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 116. | | SIQUEIRA, A. P.; OLIVEIRA, R. M. P.; SILVA FILHO, J. M.; PALHARES, M. S.; FONSECA, J. F. da; BRUSCHI, J. H.; BRUSCHI, M. C. M. Progesterona plasmática e fertilidade de fêmeas caprinas submetidas à sincronização do estro com prostaglandina F2?. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 64, n. 2, p. 305-310, 2012.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 117. | | FONSECA, J. F. da; TORRES, C. A. C.; SANTOS, A. D. F.; MAFFILI, V. V.; AMORIM, L. S.; MORAES, E. A. Progesterone and behavioral features when estrous is induced in Alpine goats. Animal Reproduction Science, v. 103, n. 3/4, p. 366-373, Jan., 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 118. | | FONSECA, J. F. da; TORRES, C. A. A.; COSTA, E. P.; MAFFILI, V. V.; CARVALHO, G. R.; ALVES, N. G.; RUBERT, M. A. Progesterone profile and reproductive performance of estrous-induced, Alpine goats given hCG fire days after breeding. Animal Reproduction, Belo Horizonte, v. 2, n. 1, p. 55-59, jan./mar. 2005.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 119. | | NASCIMENTO, P. M. P. do; BRANDÃO, F. Z.; PEREIRA, P. F. V.; PONTELLO, V. R.; TAVARES, L. L.; OLIVEIRA, A. P.; BRUSCHI, J. H.; FONSECA, J. F. da. Production of teaser female goat by short-term protocol of exogenous androgen administration. In: INTERNATIONAL CONFERENCE ON GOATS, 9.; REUNIÓN NACIONAL SOBRE CAPRINOCULTURA, 23., 2008, Querétaro, México. Sustainable goat production: challenges and opportunities of small and large enterprises; proceedings. Querétaro: International Goat Association, 2008. p. 449. Abstract 362.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 120. | | AMORIM, E. A. M. e; TORRES, C. A. A.; BRUSCHI, J. H.; FONSECA, J. F. da; GUIMARÃES, J. D.; CECON, P. R.; CARVALHO, G. R. de. Produção e composição do leite, metabólitos sangüíneos e concentração hormonal de cabras lactantes da raça Toggenburg tratadas com somatotropina bovina recombinante. Revista Brasileira de Zootecnia, Viçosa, MG, v. 35, n. 1, p. 147-153, jan./fev., 2006.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 538 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
23/08/2017 |
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Data da última atualização: |
16/05/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
NASCIMENTO, P. M. P.; SANTOS, G. B.; PENIDO, A. O.; FONSECA, J. F. da; LEITE, R. C. |
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Afiliação: |
Universidade Federal de Minas Gerais (UFMG) -Belo Horizonte, MG, Brazil; Universidade Federal Fluminense (UFF); UFMG -Belo Horizonte, MG, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; UFMG -Belo Horizonte, MG, Brazil. |
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Título: |
Identification of arthritis encephalitis caprine (CAEV) in flushing media and embryos fromnaturally infected herd goats. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Animal Reproduction, v. 12, n. 3, p. 703, Jul./Sept. 2015. |
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Idioma: |
Inglês |
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Notas: |
Proceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium, September 11th and 12th, 2015. |
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Conteúdo: |
Abstract: The goat production in Minas Gerais state has been growing in recent years, especially with intensive dairy production systems. The artificial reproduction biotechnologies are a powerful mechanism for genetics dissemination. However, the caprine arthritis encephalitis virus (CAEV) is present in most of the dairy goats farms, which represent a risk for disease transmission. The aim of this study is to identify the presence of pro-viral and viral CAEV genome in uterine lavage fluid and embryos samples collected by the non cirurgical transcervical assay. Eight goats were selected with 56.0 ± 13,4kg and a body condition score of 3.5 ± 0.75. The animals were all positive for CAEV in "Western Blotting" diagnosis. Oestrus sycronisation and ovarian superovulation was performed from adapted protocol from Fonseca et al, 2006 (Acta Scientiae Veterinariae, v. 34, sul. 1). After transcervical embryo flushing, embryos that had intact ZP, had their inner cell mass aspirated by a micromanipulator, and the product (zona pellucida and inner cell mass) was stored in RNA later. Centrifugation was performed in uterine liquid washings, until the identification of a pellet (pellet 1) and subsequently viral concentration of uterine flushings (pellet 2) was performed. The material obtained (pellets 1 and pellets 2) was submitted to viral and pro-viral genome extraction with Cador Pathogen® mini kit (Qiagen, Germany) assay. Embryonic samples (zona pellucida and inner cell mass) cDNA synthesis was performed from the viral genome with the use of M MLV enzyme according to protocol. The samples were submitted to nested PCR (Fieni, 2010,). The presence of viral and pro-viral genome in pellet (samples 2), but not in the pellet 1 or embryonic samples (zona pellucida and inner cell mass) was identified. Results shows viral replication could happen in the reproductive system from naturally infected goats, what have never been described before. However, embryos recovery from the flushing media did not show to be permissive to virus. This might indicate that the non cirurgical technique can provide virus dilution, since previous studies in embryos collected surgically revealed the presence of the virus pellet in 1, what did not happen in present work. MenosAbstract: The goat production in Minas Gerais state has been growing in recent years, especially with intensive dairy production systems. The artificial reproduction biotechnologies are a powerful mechanism for genetics dissemination. However, the caprine arthritis encephalitis virus (CAEV) is present in most of the dairy goats farms, which represent a risk for disease transmission. The aim of this study is to identify the presence of pro-viral and viral CAEV genome in uterine lavage fluid and embryos samples collected by the non cirurgical transcervical assay. Eight goats were selected with 56.0 ± 13,4kg and a body condition score of 3.5 ± 0.75. The animals were all positive for CAEV in "Western Blotting" diagnosis. Oestrus sycronisation and ovarian superovulation was performed from adapted protocol from Fonseca et al, 2006 (Acta Scientiae Veterinariae, v. 34, sul. 1). After transcervical embryo flushing, embryos that had intact ZP, had their inner cell mass aspirated by a micromanipulator, and the product (zona pellucida and inner cell mass) was stored in RNA later. Centrifugation was performed in uterine liquid washings, until the identification of a pellet (pellet 1) and subsequently viral concentration of uterine flushings (pellet 2) was performed. The material obtained (pellets 1 and pellets 2) was submitted to viral and pro-viral genome extraction with Cador Pathogen® mini kit (Qiagen, Germany) assay. Embryonic samples (zona pellucida and inner cell mass) cDNA synthes... Mostrar Tudo |
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Palavras-Chave: |
Animal biotechnology; Artrite encefalite caprina; CAEV; Caprine arthritis encephalit virus; In vivo embryos. |
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Thesagro: |
Biotecnologia; Caprino; Doença animal; Reprodução animal; Transferência de embrião; Transmissão de doença. |
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Thesaurus NAL: |
Animal diseases; Disease transmission; Embryo transfer; Goat diseases; Goats. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162905/1/CNPC-2015-Identification-of-arthritis.pdf
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Marc: |
LEADER 03618nam a2200361 a 4500
001 2074275
005 2022-05-16
008 2015 bl uuuu u00u1 u #d
100 1 $aNASCIMENTO, P. M. P.
245 $aIdentification of arthritis encephalitis caprine (CAEV) in flushing media and embryos fromnaturally infected herd goats.$h[electronic resource]
260 $aAnimal Reproduction, v. 12, n. 3, p. 703, Jul./Sept. 2015.$c2015
500 $aProceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium, September 11th and 12th, 2015.
520 $aAbstract: The goat production in Minas Gerais state has been growing in recent years, especially with intensive dairy production systems. The artificial reproduction biotechnologies are a powerful mechanism for genetics dissemination. However, the caprine arthritis encephalitis virus (CAEV) is present in most of the dairy goats farms, which represent a risk for disease transmission. The aim of this study is to identify the presence of pro-viral and viral CAEV genome in uterine lavage fluid and embryos samples collected by the non cirurgical transcervical assay. Eight goats were selected with 56.0 ± 13,4kg and a body condition score of 3.5 ± 0.75. The animals were all positive for CAEV in "Western Blotting" diagnosis. Oestrus sycronisation and ovarian superovulation was performed from adapted protocol from Fonseca et al, 2006 (Acta Scientiae Veterinariae, v. 34, sul. 1). After transcervical embryo flushing, embryos that had intact ZP, had their inner cell mass aspirated by a micromanipulator, and the product (zona pellucida and inner cell mass) was stored in RNA later. Centrifugation was performed in uterine liquid washings, until the identification of a pellet (pellet 1) and subsequently viral concentration of uterine flushings (pellet 2) was performed. The material obtained (pellets 1 and pellets 2) was submitted to viral and pro-viral genome extraction with Cador Pathogen® mini kit (Qiagen, Germany) assay. Embryonic samples (zona pellucida and inner cell mass) cDNA synthesis was performed from the viral genome with the use of M MLV enzyme according to protocol. The samples were submitted to nested PCR (Fieni, 2010,). The presence of viral and pro-viral genome in pellet (samples 2), but not in the pellet 1 or embryonic samples (zona pellucida and inner cell mass) was identified. Results shows viral replication could happen in the reproductive system from naturally infected goats, what have never been described before. However, embryos recovery from the flushing media did not show to be permissive to virus. This might indicate that the non cirurgical technique can provide virus dilution, since previous studies in embryos collected surgically revealed the presence of the virus pellet in 1, what did not happen in present work.
650 $aAnimal diseases
650 $aDisease transmission
650 $aEmbryo transfer
650 $aGoat diseases
650 $aGoats
650 $aBiotecnologia
650 $aCaprino
650 $aDoença animal
650 $aReprodução animal
650 $aTransferência de embrião
650 $aTransmissão de doença
653 $aAnimal biotechnology
653 $aArtrite encefalite caprina
653 $aCAEV
653 $aCaprine arthritis encephalit virus
653 $aIn vivo embryos
700 1 $aSANTOS, G. B.
700 1 $aPENIDO, A. O.
700 1 $aFONSECA, J. F. da
700 1 $aLEITE, R. C.
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