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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
19/02/2016 |
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Data da última atualização: |
19/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TAKETANI, R. G.; MOITINHO, M. A.; MELO, I. S. de. |
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Afiliação: |
R. G. TAKETANI, Bolsista FAPESP; M. A. MOITINHO, Bolsista CNPq; ITAMAR SOARES DE MELO, CNPMA. |
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Título: |
Metatranscriptomic and Metagenomic insights into the litter decomposition in mangroves. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1749-1. |
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Idioma: |
Português |
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Conteúdo: |
Mangroves are costal ecosystems known for its important role as sink and source of organic matter. This ecosystem covers most of the tropical and subtropical shores. A major portion of the organic matter that is produced by the mangrove vegetation is exported to nearby marine ecosystems. Therefore, this study was designed to shed light over the process of litter decomposition by using high throughput sequencing of the mRNA and DNA present in decaying mangrove leaves. With this purpose, litter bags contatining leaves of Laguncularia racemosa, Avicennia schaueriana and Rhizophora mangle were left on the mangrove sediment for 60 days. This experiment was conducted in three different mangroves in different locations. These material was had its total DNA and mRNA extracted and used to construct libraries that were sequenced in an ion torrent PGM. Sequences were annotated by MG-RAST, CAZY and Crass. The community patterns were very similar between plants; however, there were significant differences between locations. There were some important differences between the groups observed for in the DNA and mRNA libraries. The major phylogenetic groups observed were Gamma and Deltaproteobacteria while the functions were clustering-based subsystems, carbohydrate, amino acid and derivatives, protein, miscellaneous metabolism. The search for carbohydrate metabolism genes using CAZY found genes related to plant organic matter decomposition (GH26, GH17, AA3 and GH43). The major difference was a switch between Gamma and Deltaproteobacteria as dominant classes between mRNA and DNA (respectively). Another striking difference was that the DNA libraries were much more similar, despite the geographical distance than the mRNA. Hits findings highlight the importance of environmental signals to the expression of genes. MenosMangroves are costal ecosystems known for its important role as sink and source of organic matter. This ecosystem covers most of the tropical and subtropical shores. A major portion of the organic matter that is produced by the mangrove vegetation is exported to nearby marine ecosystems. Therefore, this study was designed to shed light over the process of litter decomposition by using high throughput sequencing of the mRNA and DNA present in decaying mangrove leaves. With this purpose, litter bags contatining leaves of Laguncularia racemosa, Avicennia schaueriana and Rhizophora mangle were left on the mangrove sediment for 60 days. This experiment was conducted in three different mangroves in different locations. These material was had its total DNA and mRNA extracted and used to construct libraries that were sequenced in an ion torrent PGM. Sequences were annotated by MG-RAST, CAZY and Crass. The community patterns were very similar between plants; however, there were significant differences between locations. There were some important differences between the groups observed for in the DNA and mRNA libraries. The major phylogenetic groups observed were Gamma and Deltaproteobacteria while the functions were clustering-based subsystems, carbohydrate, amino acid and derivatives, protein, miscellaneous metabolism. The search for carbohydrate metabolism genes using CAZY found genes related to plant organic matter decomposition (GH26, GH17, AA3 and GH43). The major difference was... Mostrar Tudo |
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Palavras-Chave: |
Mangrove; Metagenomic; Metatranscriptomic. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139532/1/2015RA-014.pdf
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Marc: |
LEADER 02441nam a2200169 a 4500
001 2037826
005 2016-02-19
008 2015 bl uuuu u00u1 u #d
100 1 $aTAKETANI, R. G.
245 $aMetatranscriptomic and Metagenomic insights into the litter decomposition in mangroves.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1749-1.$c1749
520 $aMangroves are costal ecosystems known for its important role as sink and source of organic matter. This ecosystem covers most of the tropical and subtropical shores. A major portion of the organic matter that is produced by the mangrove vegetation is exported to nearby marine ecosystems. Therefore, this study was designed to shed light over the process of litter decomposition by using high throughput sequencing of the mRNA and DNA present in decaying mangrove leaves. With this purpose, litter bags contatining leaves of Laguncularia racemosa, Avicennia schaueriana and Rhizophora mangle were left on the mangrove sediment for 60 days. This experiment was conducted in three different mangroves in different locations. These material was had its total DNA and mRNA extracted and used to construct libraries that were sequenced in an ion torrent PGM. Sequences were annotated by MG-RAST, CAZY and Crass. The community patterns were very similar between plants; however, there were significant differences between locations. There were some important differences between the groups observed for in the DNA and mRNA libraries. The major phylogenetic groups observed were Gamma and Deltaproteobacteria while the functions were clustering-based subsystems, carbohydrate, amino acid and derivatives, protein, miscellaneous metabolism. The search for carbohydrate metabolism genes using CAZY found genes related to plant organic matter decomposition (GH26, GH17, AA3 and GH43). The major difference was a switch between Gamma and Deltaproteobacteria as dominant classes between mRNA and DNA (respectively). Another striking difference was that the DNA libraries were much more similar, despite the geographical distance than the mRNA. Hits findings highlight the importance of environmental signals to the expression of genes.
653 $aMangrove
653 $aMetagenomic
653 $aMetatranscriptomic
700 1 $aMOITINHO, M. A.
700 1 $aMELO, I. S. de
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 64 | |
| 4. |  | FLOSS, E. L.; MOREIRA, J. C. S.; WINKELMANN, J. R. Desempenho das cultivares recomendadas de aveia, em Vacaria, RS/1988. Boletim de Pesquisa - UPF, Passo Fundo, v. 13, n. 10, p. 49-51, mar. 1990.| Biblioteca(s): Embrapa Trigo. |
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| 5. | | FONTANELI, R. S.; FLOSS, E. L.; FORCELINI, C. A.; CUNHA, M. B. Avaliação de aveia para duplo propósito, em Passo Fundo, 1981/88. (b) In: REUNIÃO DA COMISSÃO SUL-BRASILEIRA DE PESQUISA DE AVEIA, 12., 1992, Ponta Grossa. Resumos... Londrina: IAPAR, 1992. p. 25| Biblioteca(s): Embrapa Trigo. |
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| 8. | | GODOY, R.; NEGREIROS, G. DE F.; BATISTA, L. A. R.; FLOSS, E. L. Avaliação de linhagens promissoras de aveia para produção de grãos e forragem em São Carlos, SP, Brasil. São Carlos, SP: EMBRAPA-UEPAE de São Carlos, SP, 1992. 3 p. (Embrapa-UEPAE de São Carlos, SP. Pesquisa em andamento, 4).| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 9. | | FLOSS, E. L.; AUGUSTIN, L.; LINHARES, A. G.; MATIOTTI, T. G. Avaliação de genótipos de aveias silvestres, em Passo Fundo, 1998. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE AVEIA, 19., 1999, Porto Alegre. Resultados experimentais... Porto Alegre: UFRGS - Faculdade de Agronomia - Departamento de Plantas de Lavoura, 1999. p. 175-181 Editado por PACHECO, M. T.; FEDERIZZI, L. C.; MILACH, S. C. K.; MUNDSTOCK, C. M.; MARTINELLI, J. A.| Biblioteca(s): Embrapa Trigo. |
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| 19. | | SANDINI, I. E.; SÁ, J. P. G.; ROSA, J. L.; LAJÚS, C. A.; FLOSS, E. L.; GODOY, R. Ensaio nacional de aveias forrageiras, 1996: análise conjunta. In: REUNIÃO DA COMISSÃO SUL-BRASILEIRA DE PESQUISA DE AVEIA, 17., 1997. Passo Fundo. Anais... Passo Fundo: CSBPA-UFPF, 1997. p.425-426.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 64 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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