Data/hora: 21/06/2024 22:44:10

Biblioteca(s): 	Embrapa Instrumentação.	
Data corrente: 	19/11/2004	
Data da última atualização: 	08/03/2010	
Autoria: 	BUGS, M. R.; FORATO, L. A.; BORTOLETO-BUGS, R. K.; FISCHER, H.; MASCARENHAS, Y. P.; WARD, R. J.; COLNAGO, L. A.	
Título: 	Spectroscopy characterization and structural modeling of prolamin from maize and pearl millet.	
Ano de publicação: 	2004	
Fonte/Imprenta: 	European Biophysics Journal, New York, v. 33, p. 335-343, 2004.	
Idioma: 	Inglês	
Conteúdo: 	Biophysical methods and structural modeling techniques have been used to characterize the prolamins from maize (Zea mays) and pearl millet (Pennisetum americanum). The alcohol-soluble prolamin from maize, called zein, was extracted using a simple protocol and purified by gel filtration in a 70% ethanol solution. Two protein fractions were purified from seed extracts of pearl millet with molecular weights of 25.5 and 7 kDa, as estimated by SDS-PAGE. The high molecular weight protein corresponds to pennisetin, wich has a high helical content both in solution and the solid state, as demonstrated by circular dichroism and Fourier transform infrared spectra.	
Palavras-Chave: 	Dicroísmo circular; Dinâmico; Modelo molecular.	
Thesagro: 	Zeína.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Instrumentação (CNPDIA)	
Exemplares: 	CNPDIA - (8473-1) - UPC - SP - -- - PROCI-04.00027 -  - 2004.00027 - DIS