Data/hora: 23/06/2024 18:04:06

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 07/11/2019
Data da última atualização: | 07/11/2019
Autoria: | CHAIBUB, A. A.; SOUSA, T. P. de; ARAÚJO, L. G. de; FILIPPI, M. C. C. de.
Afiliação: | AMANDA A. CHAIBUB, UNB; THATYANE P. DE SOUSA, UFG; LEILA GARCES DE ARAUJO, UFG; MARTA CRISTINA CORSI DE FILIPPI, CNPAF.
Título: | Molecular and morphological characterization of rice phylloplane fungi and determination of the antagonistic activity against rice pathogens.
Ano de publicação: | 2020
Fonte/Imprenta: | Microbiological Research, v. 231, 126353, Jan. 2020.
ISSN: | 0944-5013
DOI: | 10.1016/j.micres.2019.126353
Idioma: | Inglês
Conteúdo: | Cladosporium spp. is a cosmopolitan fungal genus. In the literature, it has been reported as a biological agent for controlling several plant diseases, but its mechanism of action has never been clarified. The present study aims to identify Cladosporium spp. based on the DNA phylogeny of nine isolates obtained from the phylloplane of rice and their potential antagonistic activity against the main fungal pathogens that affect rice crop. Nine isolates of Cladosporium spp. were identified based on DNA phylogeny, molecular and morphological characterization, and their antagonistic effects with the rice pathogens C. miyabeanus, M. oryzae, M. albescens and S. oryzae. Four isolates were selected to study lytic enzymes such as &#946;-1,3-glucanase, chitinase and protease, and only one isolate was selected for a conidial germination and appressoria formation assay. The nine isolates were identified as C. cladosporioides, C. tenuissimum and C. subuliforme. Four isolates, identified as C. cladosporioides, inhibited the mycelial growth of rice pathogens such as C1H (68.59%) of S. oryzae, C5 G (74.32%) of C. miyabeanus, C11 G (75.97%) of M. oryzae and C24 G (77.39%) of M. albescens. C24 G showed a high activity of lytic enzymes, was tested against C. miyabeanus and M. oryzae, and inhibited conidial germination and appressorium formation by more than 59.36%. The characterization of C. cladosporioides suggested this species as a potential bioagent for the management of several rice diseases, especially rice blast. This is the first time that a potential biological agent from the genus Cladosporium identified at the species level was isolated from the rice phylloplane, and some of its mechanisms of action were demonstrated, such as increasing lytic enzyme activity against rice pathogens.
Thesagro: | Arroz; Doença de Planta; Fungo; Oryza Sativa; Patógeno; Taxonomia Vegetal.
Thesaurus Nal: | Cladosporium; Drug antagonism; Enzyme activity; Fungi; Mycoparasites; Rice; Taxonomy.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (35656-1) - UPC - AP - DD - 2020 -  - 2020 - DIS