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| Registros recuperados : 64 | |
| 4. |  | PÁDUA, S. B. de; ISHIKAWA, M. M.; SATAKE, F.; HISANO, H.; PIETRO, P. S. Alterações no eritrograma de surubins híbridos (Pseudoplatystoma fasciatum x P. corruscans) infectados naturalmente por Pseudomonas sp. In: CONGRESSO BRASILEIRO DE PRODUÇÃO DE PEIXES NATIVOS DE ÁGUA DOCE, 2.; ENCONTRO MATO-GROSSENSE DE AQUICULTORES, 1., 2009, Cuiabá. [Resumos...]. Cuiabá: Embrapa: Aquabio, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 6. | | ISHIKAWA, M. M.; PARENTE, B. O.; PÁDUA, S. B. de; SATAKE, F.; HISANO, H. Ocorrência de Pseudomonas sp. em surubim híbrido (Pseudoplatystoma fasciatum x P. corruscans) de piscicultura em Mato Grosso do Sul. In: CONGRESSO BRASILEIRO DE PRODUÇÃO DE PEIXES NATIVOS DE ÁGUA DOCE, 2.; ENCONTRO MATO-GROSSENSE DE AQUICULTORES, 1., 2009, Cuiabá. [Resumos...]. Cuiabá: Embrapa: Aquabio, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 9. | | ISHIKAWA, M. M.; PÁDUA, S. B. de; SATAKE, F.; MARTINS, M. L.; DIAS, M. T. Identificação morfológica de organismos semelhantes à anaplasmataceae e em monócitos de surubim híbrido (Pseudoplatystoma reticulatum x P. corruscans ) - relato de caso. Revista Brasileira de Medicina Veterinária, Rio de Janeiro, v. 33, n. 4, p. 225-228, out/dez 2011.| Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Amapá. |
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| 10. | | PÁDUA, S. B. de; PILARSKI, F.; SAKABE, R.; DIAS NETO, J.; ISHIKAWA, M. M. Heparina e K3 EDTA como anticoagulantes para tambaqui (Colossoma macropomum Cuvier, 1816). Acta Amazônica, v. 42, n. 2, p. 293-298, abr./jun., 2012.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 12. | | PADUA, S. B. de; ISHIKAWA, M. M.; SATAKE, F.; JERÔNIMO, G. T.; PILARSKI, F. First record of Trypanosoma sp. (Protozoa: Kinetoplastida) in tuvira (Gymnotus aff. inaequilabiatus) in the Pantanal wetland, Mato Grosso do Sul State, Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 20, n. 1, p. 85-87, jan/mar. 2011.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 15. | | HISANO, H.; ISHIKAWA, M. M.; MARUYAMA, M. R.; SANTANA, S. S.; PÁDUA, S. B. de. Variação de pH e da temperatura durante processo de ensilagem do resíduo de filetamento de pintado. In: AQUACIÊNCIA, 3., 2008, Maringá. Aqüicultura: desafios e inovações. Maringá: Aquabio: ITAM: UEM, Departamento de Zootecnia, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 16. | | SATAKE, F.; PADUA, S. B. de; ISHIKAWA, M. M.; LEITE, L. de O.; HONORATO, C. O. Valores de alguns constituintes bioquímicos plasmáticos do sangue de surubins híbridos (Pseudoplatystoma corruscans x Pseudoplatystoma fasciatum). In: CONGRESSO BRASILEIRO DE PRODUÇÃO DE PEIXES NATIVOS DE ÁGUA DOCE, 2.; ENCONTRO MATO-GROSSENSE DE AQUICULTORES, 1., 2009, Cuiabá. [Resumos...]. Cuiabá: Embrapa: Aquabio, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 17. | | PÁDUA, S. B. de; ISHIKAWA, M. M.; SATAKE, F.; HISANO, H.; DIAS, M. T. Valores ára o leucograma e trombograma de juvenis de Dourado (Salminus brasiliensis) em condições experimetais de cultivo. Revista Brasileira de Medicina Veterinária, Rio de Janeiro, v. 31, n. 3, p. 282-287, out./dez. 2009.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 19. | | PÁDUA, S. B. de; ISHIKAWA, M. M.; SATAKE, F.; HISANO, H.; TAVARES-DIAS, M. Valores para o leucograma e trombograma de juvenis de dourado (Salminus brasiliensis) em condições experimentais de cultivo. Revista Brasileira de Medicina Veterinária, Rio de Janeiro, v. 31, n. 4, p. 282-287, out./dez. 2009.| Biblioteca(s): Embrapa Amapá. |
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| 20. | | ROTTA, M. A.; SATAKE, F.; PÁDUA, S. B. de; ISHIKAWA, M. M.; HISANO, H. Técnica simples de marcação externa de reprodutores de tuvira (Gymnotus sp.). In: CONGRESSO BRASILEIRO DE PRODUÇÃO DE PEIXES NATIVOS DE ÁGUA DOCE, 2.; ENCONTRO MATO-GROSSENSE DE AQUICULTORES, 1., 2009, Cuiabá. [Resumos...]. Cuiabá: Embrapa: Aquabio, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| Registros recuperados : 64 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/07/2013 |
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Data da última atualização: |
19/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
PASSOS, M. A. N.; CRUZ, V. de O.; EMEDIATO, F. L.; TEIXEIRA, C. de C.; SOUZA JR. M. L T.; MATSUMOTO, T.; AZEVEDO, V. C. R.; FERREIRA, C. F.; AMORIM, E. P.; FIGUEIREDO, L. F. de A.; MARTINS, N. F.; CAVALCANTE, M. de J. B.; BAURENS, F.-C.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; PIGNOLET, L.; ABADIE, C.; CIAMPI, A. Y.; PIFFANELLI, P.; MILLER, R. N. G. |
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Afiliação: |
MARCO A. N. PASSOS, UNB; VIVIANE DE OLIVEIRA CRUZ, UNB; FLAVIA L. EMEDIATO, UNB; CRISTIANE DE CAMARGO TEIXEIRA, UCB; MANOEL T. SOUZA JR., UCB; TAKASHI MATSUMOTO, National Institute of Agrobiological Resources; VANIA CRISTINA RENNO AZEVEDO, CENARGEN; CLAUDIA FORTES FERREIRA, CNPMF; EDSON PERITO AMORIM, CNPMF; LUCIO FLAVIO DE ALENCAR FIGUEIREDO, UNB; NATALIA FLORENCIO MARTINS, CENARGEN; MARIA DE JESUS BARBOSA CAVALCANTE, DPD; FRANC-CHRISTOPHE BAURENS, CIRAD; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; GEORGIOS JOANNIS PAPPAS JUNIOR, CENARGEN; LUC PIGNOLET, CIRAD; CATHERINE ABADIE, CIRAD; ANA Y. CIAMPI; PIETRO PIFFANELLI, Genomics Platform at Parco Tecnologico Padano; ROBERT N.G. MILLER, UNB. |
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Título: |
Development of expressed sequence tag and EST-SSR marker resources for Musa acuminata. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
AOB Plants, 2012. |
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ISSN: |
2041-2851 |
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Idioma: |
Inglês |
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Notas: |
(Open Access). |
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Conteúdo: |
Background and aims: Banana (Musa acuminata) is a crop contributing to global food security. Many varieties lack resistance to biotic stresses, due to sterility and narrow genetic background. The objective of this study was to develop an expressed sequence tag (EST) database of transcripts expressed during compatible and incompatible banana-Mycosphaerella fijiensis (Mf) interactions. Black leaf streak disease (BLSD), caused by Mf, is a destructive disease of banana. Microsatellite markers were developed as a resource for crop improvement. Methodology: cDNA libraries were constructed from in vitro-infected leafes from BLSD-resistant M. acuminata ssp. burmaniccoides Calcutta 4 (MAC4) and susceptible M. acuminata cv. Cavendish Grande Naine (MACV). Clones were 5´end Sanger sequenced, ESTs assembled with TGICL and unigenes annotated using BLAST, Blast2GO and InterProScan. Mreps was used to screen for simple sequence repeats (SSRs), with markers evaluated for polymorphism using twenty diploid (AA) M. acuminata accessions contrasting in resistance to Mycosphaerella leaf spot diseases. Principal results: A total of 9333 high quality ESTs were obtained for MAC4 and 3964 for MACV, which assembled into 3995 unigenes. Of these, 2592 displayed homology to genes encoding proteins with known or putative function, and 266 to genes encoding proteins with unknown function. Gene ontology (GO) classification identified 543 GO terms, 2300 unigenes were assigned to EuKaryotic Orthologous Groups (KOG) categories and 312 mapped to KEGG pathways. A total of 624 SSR loci were identified, with trinucleotide repeat motifs the most abundant in MAC4 (54.1%) and MACV Downloaded from http://aobpla.oxfordjournals.org/ at Empresa Brasileira de Pesquisa Agropecuária on July 12, 2013 4 (57.6%). Polymorphism across M. acuminata accessions was observed with 75 markers. Alleles per polymorphic locus ranged from 2 to 8, totaling 289. Polymorphism information content (PIC) ranged from 0.08 to 0.81. Conclusions: This EST collection offers a resource for studying functional genes, including transcripts expressed in banana-Mf interactions. Markers are applicable for genetic mapping, diversity characterization and marker assisted breeding. MenosBackground and aims: Banana (Musa acuminata) is a crop contributing to global food security. Many varieties lack resistance to biotic stresses, due to sterility and narrow genetic background. The objective of this study was to develop an expressed sequence tag (EST) database of transcripts expressed during compatible and incompatible banana-Mycosphaerella fijiensis (Mf) interactions. Black leaf streak disease (BLSD), caused by Mf, is a destructive disease of banana. Microsatellite markers were developed as a resource for crop improvement. Methodology: cDNA libraries were constructed from in vitro-infected leafes from BLSD-resistant M. acuminata ssp. burmaniccoides Calcutta 4 (MAC4) and susceptible M. acuminata cv. Cavendish Grande Naine (MACV). Clones were 5´end Sanger sequenced, ESTs assembled with TGICL and unigenes annotated using BLAST, Blast2GO and InterProScan. Mreps was used to screen for simple sequence repeats (SSRs), with markers evaluated for polymorphism using twenty diploid (AA) M. acuminata accessions contrasting in resistance to Mycosphaerella leaf spot diseases. Principal results: A total of 9333 high quality ESTs were obtained for MAC4 and 3964 for MACV, which assembled into 3995 unigenes. Of these, 2592 displayed homology to genes encoding proteins with known or putative function, and 266 to genes encoding proteins with unknown function. Gene ontology (GO) classification identified 543 GO terms, 2300 unigenes were assigned to EuKaryotic Orthologous Groups (... Mostrar Tudo |
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Palavras-Chave: |
Melhoramento genético. |
|
Thesagro: |
Banana; Melhoramento vegetal; Musa Acuminata. |
|
Thesaurus NAL: |
Bananas; Breeding; Genetic improvement. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
LEADER 03489naa a2200457 a 4500
001 1961997
005 2023-05-19
008 2012 bl uuuu u00u1 u #d
022 $a2041-2851
100 1 $aPASSOS, M. A. N.
245 $aDevelopment of expressed sequence tag and EST-SSR marker resources for Musa acuminata.$h[electronic resource]
260 $c2012
500 $a(Open Access).
520 $aBackground and aims: Banana (Musa acuminata) is a crop contributing to global food security. Many varieties lack resistance to biotic stresses, due to sterility and narrow genetic background. The objective of this study was to develop an expressed sequence tag (EST) database of transcripts expressed during compatible and incompatible banana-Mycosphaerella fijiensis (Mf) interactions. Black leaf streak disease (BLSD), caused by Mf, is a destructive disease of banana. Microsatellite markers were developed as a resource for crop improvement. Methodology: cDNA libraries were constructed from in vitro-infected leafes from BLSD-resistant M. acuminata ssp. burmaniccoides Calcutta 4 (MAC4) and susceptible M. acuminata cv. Cavendish Grande Naine (MACV). Clones were 5´end Sanger sequenced, ESTs assembled with TGICL and unigenes annotated using BLAST, Blast2GO and InterProScan. Mreps was used to screen for simple sequence repeats (SSRs), with markers evaluated for polymorphism using twenty diploid (AA) M. acuminata accessions contrasting in resistance to Mycosphaerella leaf spot diseases. Principal results: A total of 9333 high quality ESTs were obtained for MAC4 and 3964 for MACV, which assembled into 3995 unigenes. Of these, 2592 displayed homology to genes encoding proteins with known or putative function, and 266 to genes encoding proteins with unknown function. Gene ontology (GO) classification identified 543 GO terms, 2300 unigenes were assigned to EuKaryotic Orthologous Groups (KOG) categories and 312 mapped to KEGG pathways. A total of 624 SSR loci were identified, with trinucleotide repeat motifs the most abundant in MAC4 (54.1%) and MACV Downloaded from http://aobpla.oxfordjournals.org/ at Empresa Brasileira de Pesquisa Agropecuária on July 12, 2013 4 (57.6%). Polymorphism across M. acuminata accessions was observed with 75 markers. Alleles per polymorphic locus ranged from 2 to 8, totaling 289. Polymorphism information content (PIC) ranged from 0.08 to 0.81. Conclusions: This EST collection offers a resource for studying functional genes, including transcripts expressed in banana-Mf interactions. Markers are applicable for genetic mapping, diversity characterization and marker assisted breeding.
650 $aBananas
650 $aBreeding
650 $aGenetic improvement
650 $aBanana
650 $aMelhoramento vegetal
650 $aMusa Acuminata
653 $aMelhoramento genético
700 1 $aCRUZ, V. de O.
700 1 $aEMEDIATO, F. L.
700 1 $aTEIXEIRA, C. de C.
700 1 $aSOUZA JR. M. L T.
700 1 $aMATSUMOTO, T.
700 1 $aAZEVEDO, V. C. R.
700 1 $aFERREIRA, C. F.
700 1 $aAMORIM, E. P.
700 1 $aFIGUEIREDO, L. F. de A.
700 1 $aMARTINS, N. F.
700 1 $aCAVALCANTE, M. de J. B.
700 1 $aBAURENS, F.-C.
700 1 $aSILVA JUNIOR, O. B. da
700 1 $aPAPPAS JUNIOR, G. J.
700 1 $aPIGNOLET, L.
700 1 $aABADIE, C.
700 1 $aCIAMPI, A. Y.
700 1 $aPIFFANELLI, P.
700 1 $aMILLER, R. N. G.
773 $tAOB Plants, 2012.
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