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| Registros recuperados : 72 | |
| 5. |  | FIGUEIREDO, J. R. de; CELESTINO, J. J. de H.; FAUSTINO, L R. Advances in artificial ovary in goats. Acta Scientiae Veterinariae, Porto Alegre, v. 38, p. s417-424, 2010. Suplemento 2. Edição dos anais da 24. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, PE, 2010.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | RODRIGUES, A. P. R.; CASTRO, S. V.; CHAVES, R. N.; FIGUEIREDO, J. R. de. Associação das técnicas de criopreservação e cultivo in vitro de folículos pré-antrais para a obtenção de embriões. Ciência Animal, Fortaleza, v. 22, n. 1, p. 42-54, 2012. suplemento. Trabalho apresentado no 6o. Congresso Norte Nordeste de Reprodução animal, 2012, Fortaleza.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 12. | | SOUSA, N. M.; CARBAYO, J. M.; FIGUEIREDO, J. R.; SULON, J.; BECKERS, J. F. Perfil de PAG (Pregnancy-Associated Glycoprotein) durante a gestação e período pós-parto e perfis de progesterona durante a gestação em cabras nativas do Nordeste do Brasil. Arquivos da Faculdade de Veterinária UFRGS, v. 25, supl, p. 304, 1997. Anais da 12a Reunião Anual da SBTE, 1997, Foz do Iguaçu.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 14. | | BEM, A. R. de; FIGUEIREDO, J. R.; SOUSA, R. V. de. Desenvolvimento de metodologia para análise de folículos ovarianos pré-antrais de bovinos em microscopia eletrônica. Arquivos da Faculdade de Veterinária UFRGS, v. 25, n. 1, p. 180, 1997. Suplemento: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TRANSFERÊNCIA DE EMBRIÕES, 12., 1997, Foz do Iguaçu, PR.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | RODRIGUES, A. P. R.; FAUSTINO, L. R.; CELESTINO, J. J. de H.; FIGUEIREDO, J. R. de. Progress in caprine and ovine preantral follicles cryopreservation. Acta Scientiae Veterinariae, Porto Alegre, v. 38, p. s425-435, 2010. Suplemento 2. Edição dos anais da 24. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, PE, 2010.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | LIMA, A. K. F.; FIGUEIREDO, J. R.; OLIVEIRA, L. C.; GONCALVES, P. B. D.; BEZERRA, M. B. Isolamento enzimático de folículos ovarianos pré-antrais ovinos: resultados preliminares. Ciência Animal, v. 7, n. 2, p. 109, jul./dez. 1997. Edição do II Simpósio Nacional de Biotecnologia da Reprodução de Animais Domésticos, 1997, Fortaleza.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | BEM, A. R. de; LUCCI, C. A.; RODRIGUES, A. P. R.; AMORIM, C. A.; FIGUEIREDO, J. R. Isolamento mecânico de folículos pré-antrais de ovários de vacas Nelore. Arquivos da Faculdade de Veterinária UFRGS, v. 25, n. 1, p. 182, 1997. Suplemento: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TRANSFERÊNCIA DE EMBRIÕES, 12., 1997, Foz do Iguaçu, PR.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | LUCCI, C. M.; AMORIM, C. A.; RODRIGUES, A. P. R.; FIGUEIREDO, J. R.; GONÇALVES, P. B. D. Isolamento mecânico de folículos pré-antrais a partir de ovários de cabras em diferentes estágios reprodutivos: resultados preliminares. Arquivos da Faculdade de Veterinária UFRGS, v. 26, n. 1, p. 304, 1998. Suplemento. Resumo apresentado na 13a. Reunião Anual da Sociedade Brasileira de Transferência de Embriões, 1998, Atibaia.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | AMORIM, C. A.; RODRIGUES, A. P. R.; LUCCI, C. M.; FIGUEIREDO, J. R.; GONÇALVES, P. B. D. Método mecânico para o isolamento de folículos pré-antrais a partir de ovários de ovelhas adultas. Arquivos da Faculdade de Veterinária UFRGS, v. 26, n. 1, p. 214, 1998. Suplemento. Resumo apresentado na 13a. Reunião Anual da Sociedade Brasileira de Transferência de Embriões, 1998, Atibaia.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | ANDRADE, E. R.; SENEDA, M. M.; ALFIERI, A. A.; OLIVEIRA, J. A.; FIGUEIREDO, J. R.; TONIOLLI, R. Efeito da concentração de ácido 3-indol-acético na ativação e crescimento in vitro de folículos pré-antrais ovinos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 57, n. 3, p. 334-339, jun. 2005.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 72 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
07/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PAES, V. M.; VIEIRA, L. A.; CORREIA, H. H. V.; SA, N. A. R.; MOURA, A. A. A.; SALES, A. D.; RODRIGUES, A. P. R.; MAGALHÃES-PADILHA, D. M.; SANTOS, F. W.; APGAR, G. A.; CAMPELLO, C. C.; CAMARGO, L. S. de A.; FIGUEIREDO, J. R. |
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Afiliação: |
V. M. Paes, LAMOFOPA - CE; L. A. Vieira, LAMOFOPA - CE; H. H. V. Correia, LAMOFOPA - CE; N. A. R. Sa, LAMOFOPA - CE; A. A. A. Moura, Universidade Federal do Ceará; A. D. Sales, LAMOFOPA - CE; A. P. R. Rodrigues, LAMOFOPA - CE; D. M. Magalhães-Padilha, Universidade Potiguar; F. W. Santos, Universidade Estadual do Pampa; G. A. Apgar, Southern Illinois University, Carbondale, Illinois, USA; C. C. Campello, LAMOFOPA - CE; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; J. R. Figueiredo, LAMOFOPA - CE. |
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Título: |
Effect of heat stress on the survival and development of in vitro cultured bovine preantral follicles and on in vitro maturation of cumulus-oocyte complex. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Theriogenology, n. 86, p. 994-1003, 2016. |
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Idioma: |
Português |
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Conteúdo: |
Abstract The deleterious effect of heat stress (HS) on competence of oocytes from antral follicles is well recognized, but there is a lack of data regarding its impact on the viability and growth of preantral follicles. In this study, we used in vitro preantral follicle cultures to investigate the effects of HS on the following parameters: survival and development of primordial follicles after in vitro culture of ovarian fragments (experiment I); growth and antrum formation of isolated advanced secondary follicles (experiment II); and maturation rates after in vitro maturation (IVM) of cumulus?oocyte complexes (COCs) from antral follicles (>2?6 mm) grown in vivo (experiment III). Furthermore, the following end points were evaluated in all experiments: follicle/oocyte survival, reactive oxygen species (ROS), estradiol (E2) and progesterone (P4) production, as well asmRNA expression for select genes related to stress (HSP70) and apoptosis (MCL1 and BAX). In all experiments, HS consisted of exposing the structures (ovarian fragments, isolated preantral follicles and COCs) to 41 C for 12 hours and then to 38.5 C until the end of the culture (7 days for experiments I and II and 24 hours for experiment III). The temperature for the control group was held at 38.5 C for the entire culture period. Heat stress increased (P < 0.05) the percentage of developing follicles (intermediate, primary, and secondary follicles) at 12 hours and increased levels of ROS at all evaluated time points (12, 24 hours, and D7), when compared to the control (experiment I). Heat stress did not affect (P > 0.05) any identified end points when preantral follicles were cultured in their isolated form (experiment II). However, in experiment III, HS decreased (P < 0.05) both the rates of metaphase II after 24 hours and E2 production at 12 hours of IVM. Moreover, HS increased (P < 0.0001) levels of P4 after IVM and ROS production at every evaluated time point, compared with the control (12 and 24 hours). In conclusion, HS caused: (1) early activation of primordial follicles; (2) an increase in ROS production by early preantral follicles enclosed in ovarian tissue and by COCs; (3) a short-term reduction of E2 production by COCs; and (4) an increase in P4 secretion from COCs. However, HS did not affectin vitro culture of advanced isolated secondary follicles. Experimental evidence indicates that preantral follicles are less sensitive to HS than COC. MenosAbstract The deleterious effect of heat stress (HS) on competence of oocytes from antral follicles is well recognized, but there is a lack of data regarding its impact on the viability and growth of preantral follicles. In this study, we used in vitro preantral follicle cultures to investigate the effects of HS on the following parameters: survival and development of primordial follicles after in vitro culture of ovarian fragments (experiment I); growth and antrum formation of isolated advanced secondary follicles (experiment II); and maturation rates after in vitro maturation (IVM) of cumulus?oocyte complexes (COCs) from antral follicles (>2?6 mm) grown in vivo (experiment III). Furthermore, the following end points were evaluated in all experiments: follicle/oocyte survival, reactive oxygen species (ROS), estradiol (E2) and progesterone (P4) production, as well asmRNA expression for select genes related to stress (HSP70) and apoptosis (MCL1 and BAX). In all experiments, HS consisted of exposing the structures (ovarian fragments, isolated preantral follicles and COCs) to 41 C for 12 hours and then to 38.5 C until the end of the culture (7 days for experiments I and II and 24 hours for experiment III). The temperature for the control group was held at 38.5 C for the entire culture period. Heat stress increased (P < 0.05) the percentage of developing follicles (intermediate, primary, and secondary follicles) at 12 hours and increased levels of ROS at all evaluated time points... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Oocyte; Preantral follicle. |
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Thesaurus NAL: |
heat stress; in vitro culture. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03440naa a2200325 a 4500
001 2062969
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aPAES, V. M.
245 $aEffect of heat stress on the survival and development of in vitro cultured bovine preantral follicles and on in vitro maturation of cumulus-oocyte complex.$h[electronic resource]
260 $c2016
520 $aAbstract The deleterious effect of heat stress (HS) on competence of oocytes from antral follicles is well recognized, but there is a lack of data regarding its impact on the viability and growth of preantral follicles. In this study, we used in vitro preantral follicle cultures to investigate the effects of HS on the following parameters: survival and development of primordial follicles after in vitro culture of ovarian fragments (experiment I); growth and antrum formation of isolated advanced secondary follicles (experiment II); and maturation rates after in vitro maturation (IVM) of cumulus?oocyte complexes (COCs) from antral follicles (>2?6 mm) grown in vivo (experiment III). Furthermore, the following end points were evaluated in all experiments: follicle/oocyte survival, reactive oxygen species (ROS), estradiol (E2) and progesterone (P4) production, as well asmRNA expression for select genes related to stress (HSP70) and apoptosis (MCL1 and BAX). In all experiments, HS consisted of exposing the structures (ovarian fragments, isolated preantral follicles and COCs) to 41 C for 12 hours and then to 38.5 C until the end of the culture (7 days for experiments I and II and 24 hours for experiment III). The temperature for the control group was held at 38.5 C for the entire culture period. Heat stress increased (P < 0.05) the percentage of developing follicles (intermediate, primary, and secondary follicles) at 12 hours and increased levels of ROS at all evaluated time points (12, 24 hours, and D7), when compared to the control (experiment I). Heat stress did not affect (P > 0.05) any identified end points when preantral follicles were cultured in their isolated form (experiment II). However, in experiment III, HS decreased (P < 0.05) both the rates of metaphase II after 24 hours and E2 production at 12 hours of IVM. Moreover, HS increased (P < 0.0001) levels of P4 after IVM and ROS production at every evaluated time point, compared with the control (12 and 24 hours). In conclusion, HS caused: (1) early activation of primordial follicles; (2) an increase in ROS production by early preantral follicles enclosed in ovarian tissue and by COCs; (3) a short-term reduction of E2 production by COCs; and (4) an increase in P4 secretion from COCs. However, HS did not affectin vitro culture of advanced isolated secondary follicles. Experimental evidence indicates that preantral follicles are less sensitive to HS than COC.
650 $aheat stress
650 $ain vitro culture
653 $aBovine
653 $aOocyte
653 $aPreantral follicle
700 1 $aVIEIRA, L. A.
700 1 $aCORREIA, H. H. V.
700 1 $aSA, N. A. R.
700 1 $aMOURA, A. A. A.
700 1 $aSALES, A. D.
700 1 $aRODRIGUES, A. P. R.
700 1 $aMAGALHÃES-PADILHA, D. M.
700 1 $aSANTOS, F. W.
700 1 $aAPGAR, G. A.
700 1 $aCAMPELLO, C. C.
700 1 $aCAMARGO, L. S. de A.
700 1 $aFIGUEIREDO, J. R.
773 $tTheriogenology$gn. 86, p. 994-1003, 2016.
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