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| 83. | | DORNELAS, J. C. M.; FIGUEIREDO, J. E. F.; GOMES, E. A.; OLIVEIRA-PAIVA, C. A.; LANA, U. G. de P.; MARRIEL, I. E.; STORANOFF, M. A. R. Identificação e atividade antogonista in vitro de actinobactérias para o biocontrole de Fusarium verticilioides, agente causal da podridão do colmo em milho (Zea mays L.). In: CONGRESO LATINOAMERICANO DE MICROBIOLOGIA, 24.; CONGRESO CHILENO DE MICROBIOLOGIA, 40.; REUNIÓN ANUAL DE LA ASSOCIACIÓN CHILENA DE INMUNOLOGIA, 2.; REUNIÓN DE LA SOCIEDAD LATINOAMERICANA DE TUBERCULOSIS Y OTRAS MICOBACTERIOSIS, 9., 2018, Santiago, Chile. Libro de resúmenes. Santiago: Asociación Latinoamericana de Microbiología, 2018. p. 1142.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 84. | | VASCONCELOS, M. J. V.; ANTUNES, M. S.; BARDUCHE, D.; PAIVA, L. V.; PAIVA, E.; VALICENTE, F. H.; FIGUEIREDO, J. E. F.; CARVALHO, C. H. S.; LOPES, M. A. Genetic transformation of tropical maize (Zea mays L.) using Agrobacterium and bombardment. In: INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 6., 1998, San Diego. Final program e abstracts guide. New York: SCHERAGO International, 1998. p. 173.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 85. | | VASCONCELOS, M. J. V. de; JAIN, A.; FIGUEIREDO, J. E. F.; OLIVEIRA, M. F. de; TRINDADE, R. dos S.; YUGANDHAR, P.; RAGHOTHAMA, K. G. Gaspé Flint corn as a seed-to-seed model to study the effect of phosphorus on maize growth and development. Genetics and Molecular Research, v. 23, n. 1, gmr19213, 2024.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 87. | | FIGUEIREDO, J. E. F.; ROCHA, T. L.; FONTES, M. A.; CRUZ, I.; KALAPOTHAKIS, E.; GOMEZ, M. V.; PURCINO, A. A. C. Expressão da neurotoxina TX4(6-1) em sistema heterólogo e bioensaio com a lagarta do cartucho (Spodoptera frugiperda). In: CONGRESSO NACIONAL DE MILHO E SORGO, 23., 2000, Uberlândia, MG. A inovação tecnológica e a competividade no contexto dos mercados globalizados: resumos expandidos. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Uberlândia: Universidade Federal de Uberlandia, 2000. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 88. | | FIGUEIREDO, J. E. F.; ROCHA, T. L.; FONTES, M. A.; CRUZ, I.; KALAPOTHAKIS, E.; GOMEZ, M. V.; PURCINO, A. A. C. Expressão da neurotoxina TX4-(6-1) em sistema heterólogo e bioensaio com a lagarta-do-cartucho (Spodoptera frugiperda). In: CONGRESSO NACIONAL DE MILHO E SORGO, 23., 2000, Uberlândia. A inovação tecnológica e a competividade no contexto dos mercados globalizados: resumos. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Uberlândia: Universidade Federal de Uberlândia, 2000. p. 159.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 90. | | LANZA, F. E.; ZAMBOLIM, L.; CASELA, C. R.; COSTA, R. V.; COTA, L. V.; SILVA, D. D.; FIGUEIREDO, J. E. F. Etiology and epidemiological variables associated with maize resistance to white spot disease. Journal of Plant Pathology, v. 95, n. 2, p. 349-359, 2013.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 91. | | GONÇALVES, R. M.; FIGUEIREDO, J. E. F.; PEDRO, E. S.; MEIRELLES, W. F.; LEITE JÚNIOR, R. P.; SAUER, A. V.; PACCOLA-MEIRELLES, L. D. Etiology and Phaeosphaeria leaf spot disease of maize. Journal of Plant Pathology, v. 95, n. 3, p. 559-569, 2013.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 92. | | OLIVEIRA-PAIVA, C. A.; CALAZANS, G. M.; FIGUEIREDO, J. E. F.; MENDES, S. M.; GOMES, E. A.; MARUCCI, R. C.; VIANA, P. A.; SELDIN, L.; MARRIEL, I. E. Microbial activities in soils cultivated with transgenic maize expressing Bacillus thuringiensis Cry1 Ab and Cry1 F genes. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 14, n. 3, p. 409-419, 2015.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 93. | | FIGUEIREDO, J. E. F.; GOMES, E. A.; GUIMARAES, C. T.; LANA, U. G. de P.; TEIXEIRA, M. A.; LIMA, G. V. C.; BRESSAN, W. Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.). Brazilian Journal of Microbiology, São Paulo, v. 40, n. 3, p. 522-534, 2009.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 94. | | PEDRO, E. dos S.; GONÇALVES, R. M.; FIGUEIREDO, J. E. F.; MEIRELLES, W. F.; COSTA, R. V.; COTA, L. V.; SILVA, D. D. da; PACCOLA-MEIRELLES, L. D. Nova evidência comprovando ser a bactéria Pantoea ananatis o agente etiológico da mancha-branca-do-milho (mancha-de-phaeosphaeria). Sete Lagoas: Embrapa Milho e Sorgo, 2013. 20 p. (Embrapa Milho e Sorgo. Boletim de Pesquisa e Desenvolvimento, 80).| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 95. | | SOUZA-PACCOLA, E. A de; BOMFETI, C. A.; TANAKA, F. A. O.; MASSOLA JUNIOR, N. S.; COLAUTO, N. B.; FIGUEIREDO, J. E. F.; PACCOLA-MEIRELLES, L. D. Novel insights into the early stages of infection by oval conidia of Colletotrichum sublineolum. Scientia Agricola, Piracicaba, v. 72, n. 4, p. 351-355, July/Aug. 2015.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 97. | | OLIVEIRA, M. C.; SILVA, A. O.; FERNANDES, T. A.; VALICENTE, F. H.; GOMES, E. A.; FIGUEIREDO, J. E. F.; OLIVEIRA-PAIVA, C. A. Long-term preservation and data management of the Embrapa Milho e Sorgo Bacillus thuringiensis culture collection. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 29.; ENCONTRO NACIONAL DE MICROBIOLOGIA AMBIENTAL, 15., 2017, Foz do Iguaçu. Resumos... São Paulo: Sociedade Brasileira de Microbiologia, 2017.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 100. | | FIGUEIREDO, J. E. F.; SILVA, J. A. A.; LIMA, G. V. C.; SILVA, C. H. C.; MOURÃO, S. A.; LOPES, S. C.; KARAM, D. Efeito in vitro de herbicidas sobre bactérias da rizosfera de sempre viva (Syngonanthus Elegans var. elegans). In: CONGRESSO DE ECOLOGIA DO BRASIL, 10.; SIMPÓSIO DE SUSTENTABILIDADE, 1., 2011, São Lourenço. Sustentabilidade: anais. São Paulo: Sociedade de Ecologia do Brasil, 2011. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
03/01/2014 |
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Data da última atualização: |
27/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
GONÇALVES, R. M.; FIGUEIREDO, J. E. F.; PEDRO, E. S.; MEIRELLES, W. F.; LEITE JÚNIOR, R. P.; SAUER, A. V.; PACCOLA-MEIRELLES, L. D. |
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Afiliação: |
JOSE EDSON FONTES FIGUEIREDO, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS. |
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Título: |
Etiology and Phaeosphaeria leaf spot disease of maize. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Plant Pathology, v. 95, n. 3, p. 559-569, 2013. |
|
DOI: |
10.4454/JPP.V95I3.037 |
|
Idioma: |
Inglês |
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Conteúdo: |
Different fungal species and the bacterium Pantoea ananatis (Pa) have been reported as etiological agents of Phaeosphaeria leaf spot (PLS) disease. This work aimed at using molecular identification of the fungi and bacteria occurring in PLS and its etiology. Genomic DNAs from (i) pool of each of the four stages of PLS lesions; (ii) bacteria and fungi isolated from lesions of natural PLS (NPLS) and artificial injuries (AI); (iii) fungi isolated from lesions obtained from plants inoculated with Pain greenhouse (GH) were used in PCR with universal primers for bacteria and fungal rRNA genes, and species-specific primers for Pa. Bacterial amplicons were observed at all stages of lesions and fungal amplicons in stages 3 and 4. Bacterial amplicons of pooled NPLS lesions were from Pawhile fungal amplicons were from Phaeosphaeria sp. and Phoma sp. Bacteria from NPLS, GH and AI lesions were identified as Pa, Pa and Bacillus subtilis, respectively, while the fungi were Epicoccum nigrum, Leptosphaeria sacchari, Cochliobolus geniculatus, Pithomyces chartarum, Alternaria alternata, A. ricini, Gibberella intricans, G. fujikuroi, Phaeosphaeria sp., P. avenaria, Phoma sp., Phyllosticta sp., Sarocladium strictum, Glomerella graminicola, and Cochliobolus heterostrophus. Symptoms of PLS decreased by 90% with the use of oxytetracycline in foliar treatment of maize plants in the field, and its addition to culture medium completely inhibited the growth of Pa. The results strongly show that Pa is the causal agent of PLS disease and different species of opportunistic fungi appear late in the necrotic stages of lesions caused by Pa. MenosDifferent fungal species and the bacterium Pantoea ananatis (Pa) have been reported as etiological agents of Phaeosphaeria leaf spot (PLS) disease. This work aimed at using molecular identification of the fungi and bacteria occurring in PLS and its etiology. Genomic DNAs from (i) pool of each of the four stages of PLS lesions; (ii) bacteria and fungi isolated from lesions of natural PLS (NPLS) and artificial injuries (AI); (iii) fungi isolated from lesions obtained from plants inoculated with Pain greenhouse (GH) were used in PCR with universal primers for bacteria and fungal rRNA genes, and species-specific primers for Pa. Bacterial amplicons were observed at all stages of lesions and fungal amplicons in stages 3 and 4. Bacterial amplicons of pooled NPLS lesions were from Pawhile fungal amplicons were from Phaeosphaeria sp. and Phoma sp. Bacteria from NPLS, GH and AI lesions were identified as Pa, Pa and Bacillus subtilis, respectively, while the fungi were Epicoccum nigrum, Leptosphaeria sacchari, Cochliobolus geniculatus, Pithomyces chartarum, Alternaria alternata, A. ricini, Gibberella intricans, G. fujikuroi, Phaeosphaeria sp., P. avenaria, Phoma sp., Phyllosticta sp., Sarocladium strictum, Glomerella graminicola, and Cochliobolus heterostrophus. Symptoms of PLS decreased by 90% with the use of oxytetracycline in foliar treatment of maize plants in the field, and its addition to culture medium completely inhibited the growth of Pa. The results strongly show that Pa is t... Mostrar Tudo |
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Palavras-Chave: |
Plant diseases. |
|
Thesagro: |
Doença de planta; Milho; Zea mays. |
|
Thesaurus NAL: |
Foliar diseases; Pantoea ananatis; Polymerase chain reaction. |
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Categoria do assunto: |
H Saúde e Patologia |
|
Marc: |
LEADER 02465naa a2200289 a 4500
001 1974841
005 2017-09-27
008 2013 bl uuuu u00u1 u #d
024 7 $a10.4454/JPP.V95I3.037$2DOI
100 1 $aGONÇALVES, R. M.
245 $aEtiology and Phaeosphaeria leaf spot disease of maize.$h[electronic resource]
260 $c2013
520 $aDifferent fungal species and the bacterium Pantoea ananatis (Pa) have been reported as etiological agents of Phaeosphaeria leaf spot (PLS) disease. This work aimed at using molecular identification of the fungi and bacteria occurring in PLS and its etiology. Genomic DNAs from (i) pool of each of the four stages of PLS lesions; (ii) bacteria and fungi isolated from lesions of natural PLS (NPLS) and artificial injuries (AI); (iii) fungi isolated from lesions obtained from plants inoculated with Pain greenhouse (GH) were used in PCR with universal primers for bacteria and fungal rRNA genes, and species-specific primers for Pa. Bacterial amplicons were observed at all stages of lesions and fungal amplicons in stages 3 and 4. Bacterial amplicons of pooled NPLS lesions were from Pawhile fungal amplicons were from Phaeosphaeria sp. and Phoma sp. Bacteria from NPLS, GH and AI lesions were identified as Pa, Pa and Bacillus subtilis, respectively, while the fungi were Epicoccum nigrum, Leptosphaeria sacchari, Cochliobolus geniculatus, Pithomyces chartarum, Alternaria alternata, A. ricini, Gibberella intricans, G. fujikuroi, Phaeosphaeria sp., P. avenaria, Phoma sp., Phyllosticta sp., Sarocladium strictum, Glomerella graminicola, and Cochliobolus heterostrophus. Symptoms of PLS decreased by 90% with the use of oxytetracycline in foliar treatment of maize plants in the field, and its addition to culture medium completely inhibited the growth of Pa. The results strongly show that Pa is the causal agent of PLS disease and different species of opportunistic fungi appear late in the necrotic stages of lesions caused by Pa.
650 $aFoliar diseases
650 $aPantoea ananatis
650 $aPolymerase chain reaction
650 $aDoença de planta
650 $aMilho
650 $aZea mays
653 $aPlant diseases
700 1 $aFIGUEIREDO, J. E. F.
700 1 $aPEDRO, E. S.
700 1 $aMEIRELLES, W. F.
700 1 $aLEITE JÚNIOR, R. P.
700 1 $aSAUER, A. V.
700 1 $aPACCOLA-MEIRELLES, L. D.
773 $tJournal of Plant Pathology$gv. 95, n. 3, p. 559-569, 2013.
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