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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
08/01/2001 |
Data da última atualização: |
08/01/2001 |
Autoria: |
YANG, C. -H.; CROWLEY, D. E. |
Título: |
Rhizosphere microbial community structure in relation to root location and plant iron nutritional status. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 66, p. 345-351, 2000. |
Idioma: |
Inglês |
Conteúdo: |
Root exudate composition and quantity vary in relation to plant nutritional status, but the impact of the differences on rhizosphere microbial communities is not known. To examine this question, we performed an experiment with barley (Hordeum vulgare) plants under iron-limiting and iron-sufficient growth conditions. Plants were grown in an iron-limiting soil in root box microcosms. One-half of the plants were treated with foliar iron every day to inhibit phytosiderophore production and to alter root exudate composition. After 30 days, the bacterial communities associated with different root zones, including the primary root tips, nonelongating secondary root tips, sites of lateral root emergence, and older roots distal from the tip, were characterized by using 16S ribosomal DNA (rDNA) fingerprints generated by PCR-denaturing gradient gel electrophoresis (DGGE). Our results showed that the microbial communities associated with the different root locations produced many common 16S rDNA bands but that the communities could be distinguished by using correspondence analysis. Approximately 40% of the variation between communities could be attributed to plant iron nutritional status. A sequence analysis of clones generated from a single 16S rDNA band obtained at all of the root locations revealed that there were taxonomically different species in the same band, suggesting that the resolving power of DGGE for characterization of community structure at the species levels is limited. Our results suggest that the bacterial communities in the rhizosphere are substantially different in different root zones and that a rhizosphere community may be altered by changes in root exudate composition caused by changes in plant iron nutritional status. MenosRoot exudate composition and quantity vary in relation to plant nutritional status, but the impact of the differences on rhizosphere microbial communities is not known. To examine this question, we performed an experiment with barley (Hordeum vulgare) plants under iron-limiting and iron-sufficient growth conditions. Plants were grown in an iron-limiting soil in root box microcosms. One-half of the plants were treated with foliar iron every day to inhibit phytosiderophore production and to alter root exudate composition. After 30 days, the bacterial communities associated with different root zones, including the primary root tips, nonelongating secondary root tips, sites of lateral root emergence, and older roots distal from the tip, were characterized by using 16S ribosomal DNA (rDNA) fingerprints generated by PCR-denaturing gradient gel electrophoresis (DGGE). Our results showed that the microbial communities associated with the different root locations produced many common 16S rDNA bands but that the communities could be distinguished by using correspondence analysis. Approximately 40% of the variation between communities could be attributed to plant iron nutritional status. A sequence analysis of clones generated from a single 16S rDNA band obtained at all of the root locations revealed that there were taxonomically different species in the same band, suggesting that the resolving power of DGGE for characterization of community structure at the species levels is limited. ... Mostrar Tudo |
Thesagro: |
Cevada; Hordeum Vulgare; Raiz; Rizosfera; Sideróforo. |
Thesaurus Nal: |
barley; rhizosphere; roots; siderophores. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02426naa a2200241 a 4500 001 1621190 005 2001-01-08 008 2000 bl --- 0-- u #d 100 1 $aYANG, C. -H. 245 $aRhizosphere microbial community structure in relation to root location and plant iron nutritional status. 260 $c2000 520 $aRoot exudate composition and quantity vary in relation to plant nutritional status, but the impact of the differences on rhizosphere microbial communities is not known. To examine this question, we performed an experiment with barley (Hordeum vulgare) plants under iron-limiting and iron-sufficient growth conditions. Plants were grown in an iron-limiting soil in root box microcosms. One-half of the plants were treated with foliar iron every day to inhibit phytosiderophore production and to alter root exudate composition. After 30 days, the bacterial communities associated with different root zones, including the primary root tips, nonelongating secondary root tips, sites of lateral root emergence, and older roots distal from the tip, were characterized by using 16S ribosomal DNA (rDNA) fingerprints generated by PCR-denaturing gradient gel electrophoresis (DGGE). Our results showed that the microbial communities associated with the different root locations produced many common 16S rDNA bands but that the communities could be distinguished by using correspondence analysis. Approximately 40% of the variation between communities could be attributed to plant iron nutritional status. A sequence analysis of clones generated from a single 16S rDNA band obtained at all of the root locations revealed that there were taxonomically different species in the same band, suggesting that the resolving power of DGGE for characterization of community structure at the species levels is limited. Our results suggest that the bacterial communities in the rhizosphere are substantially different in different root zones and that a rhizosphere community may be altered by changes in root exudate composition caused by changes in plant iron nutritional status. 650 $abarley 650 $arhizosphere 650 $aroots 650 $asiderophores 650 $aCevada 650 $aHordeum Vulgare 650 $aRaiz 650 $aRizosfera 650 $aSideróforo 700 1 $aCROWLEY, D. E. 773 $tApplied and Environmental Microbiology, Washington$gv. 66, p. 345-351, 2000.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
02/10/1997 |
Data da última atualização: |
01/09/2021 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
BELLAVER, C.; FIGUEIREDO, E. A. P. de; OLIVEIRA, E. R. de. |
Afiliação: |
CLÁUDIO BELLAVER, CNPC; ÉLSIO ANTÔNIO PEREIRA DE FIGUEIREDO, CNPC; EDERLON RIBEIRO DE OLIVERIA, CNPC. |
Título: |
Determinação do rendimento de carcaças fria e relação porção comestível/osso em caprinos e ovinos tropicais. |
Ano de publicação: |
1980 |
Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 17., 1980, Fortaleza. Anais... Fortaleza: Sociedade Brasileira de Zootecnia, 1980. p. 214. |
Idioma: |
Português |
Thesagro: |
Caprino; Carcaça; Carne; Ovino; Rendimento. |
Thesaurus NAL: |
Goat meat; Goats; Meat; Ovine carcasses; Sheep; Sheep meat; Small ruminants; Yield components. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/35867/1/RAC-1980-5.pdf
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Marc: |
LEADER 00887nam a2200277 a 4500 001 1514804 005 2021-09-01 008 1980 bl uuuu u00u1 u #d 100 1 $aBELLAVER, C. 245 $aDeterminação do rendimento de carcaças fria e relação porção comestível/osso em caprinos e ovinos tropicais.$h[electronic resource] 260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 17., 1980, Fortaleza. Anais... Fortaleza: Sociedade Brasileira de Zootecnia, 1980. p. 214.$c1980 650 $aGoat meat 650 $aGoats 650 $aMeat 650 $aOvine carcasses 650 $aSheep 650 $aSheep meat 650 $aSmall ruminants 650 $aYield components 650 $aCaprino 650 $aCarcaça 650 $aCarne 650 $aOvino 650 $aRendimento 700 1 $aFIGUEIREDO, E. A. P. de 700 1 $aOLIVEIRA, E. R. de
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Embrapa Caprinos e Ovinos (CNPC) |
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