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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
03/06/2023 |
Data da última atualização: |
22/08/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
OLIVEIRA, C. S.; ROSA, P. M. da S.; ALVES, B. R. C.; MONTEIRO, C. A. S.; LEAL, G. R.; GUEDES, P. H. E.; CAMARGO, A. J. dos R.; SARAIVA, N. Z. |
Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; PAOLA MARIA DA SILVA ROSA; BRUNA RIOS COELHO ALVES; CLARA ANA SANTOS MONTEIRO; GABRIELA RAMOS LEAL; PEDRO HENRIQUE EVANGELISTA GUEDES; AGOSTINHO JORGE DOS REIS CAMARGO; NAIARA ZOCCAL SARAIVA, CNPGL. |
Título: |
Luteinizing hormone secretion, ovulatory capacity, and oocyte quality in peripubertal Gir heifers. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Zygote, v. 31, n. 4, p. 342-349, 2023. |
DOI: |
https://doi.org/10.1017/S0967199423000230 |
Idioma: |
Inglês |
Conteúdo: |
Induction of puberty in cattle breeds that attain puberty in later stages, such as Gir, allows the earlier beginning of reproductive life and it might increase oocyte quality. Here, the ovulatory capacity of prepuberal Gir heifers was studied and its relationship to follicular growth, luteinizing hormone (LH) secretion and oocyte quality was evaluated. Peripubertal Gir heifers were treated with a progesterone-based protocol and according to ovulatory response were separated into groups: not-ovulated (N-OV) and ovulated (OV). Serial blood samples were taken 24 h after estradiol treatment on day 12 to evaluate LH secretion. Cumulus-oocyte complexes (COCs) were collected using ovum pick-up and assessed for brilliant cresyl blue (BCB) staining rate, IVF-grade oocytes rate, and mean oocyte diameter, in comparison with cow oocytes. Gene expression of developmental competence markers (ZAR1, MATER, and IGF2R) was also analyzed. The largest follicle diameters were similar between N-OV and OV groups on the day of estradiol treatment (d12) and the next day and decreased (P = 0.04) in the N-OV group thereafter. LH pulse secretion was different between groups (N-OV = 3.61 +/- 0.34 vs OV = 2.83 +/- 0.21 ng/ ml; P = 0.04). COC assessment showed that the number of recovered oocytes, BCB+ rate, IVF-grade oocytes and oocyte size was similar (P > 0.05) among groups, resembling adult cow patterns. ZAR1, MATER and IGF2R gene expression in oocytes were also similar (P > 0.05) in N-OV and OV groups. In conclusion, our results demonstrate a lower LH secretion profile in peripubertal Gir heifers prone to ovulate after induction protocol, and that oocyte quality is not affected on a short-term basis by ovulation itself. MenosInduction of puberty in cattle breeds that attain puberty in later stages, such as Gir, allows the earlier beginning of reproductive life and it might increase oocyte quality. Here, the ovulatory capacity of prepuberal Gir heifers was studied and its relationship to follicular growth, luteinizing hormone (LH) secretion and oocyte quality was evaluated. Peripubertal Gir heifers were treated with a progesterone-based protocol and according to ovulatory response were separated into groups: not-ovulated (N-OV) and ovulated (OV). Serial blood samples were taken 24 h after estradiol treatment on day 12 to evaluate LH secretion. Cumulus-oocyte complexes (COCs) were collected using ovum pick-up and assessed for brilliant cresyl blue (BCB) staining rate, IVF-grade oocytes rate, and mean oocyte diameter, in comparison with cow oocytes. Gene expression of developmental competence markers (ZAR1, MATER, and IGF2R) was also analyzed. The largest follicle diameters were similar between N-OV and OV groups on the day of estradiol treatment (d12) and the next day and decreased (P = 0.04) in the N-OV group thereafter. LH pulse secretion was different between groups (N-OV = 3.61 +/- 0.34 vs OV = 2.83 +/- 0.21 ng/ ml; P = 0.04). COC assessment showed that the number of recovered oocytes, BCB+ rate, IVF-grade oocytes and oocyte size was similar (P > 0.05) among groups, resembling adult cow patterns. ZAR1, MATER and IGF2R gene expression in oocytes were also similar (P > 0.05) in N-OV and OV group... Mostrar Tudo |
Palavras-Chave: |
Competência oocitária; Puberdade. |
Thesagro: |
Bovino. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02495naa a2200253 a 4500 001 2154200 005 2023-08-22 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1017/S0967199423000230$2DOI 100 1 $aOLIVEIRA, C. S. 245 $aLuteinizing hormone secretion, ovulatory capacity, and oocyte quality in peripubertal Gir heifers.$h[electronic resource] 260 $c2023 520 $aInduction of puberty in cattle breeds that attain puberty in later stages, such as Gir, allows the earlier beginning of reproductive life and it might increase oocyte quality. Here, the ovulatory capacity of prepuberal Gir heifers was studied and its relationship to follicular growth, luteinizing hormone (LH) secretion and oocyte quality was evaluated. Peripubertal Gir heifers were treated with a progesterone-based protocol and according to ovulatory response were separated into groups: not-ovulated (N-OV) and ovulated (OV). Serial blood samples were taken 24 h after estradiol treatment on day 12 to evaluate LH secretion. Cumulus-oocyte complexes (COCs) were collected using ovum pick-up and assessed for brilliant cresyl blue (BCB) staining rate, IVF-grade oocytes rate, and mean oocyte diameter, in comparison with cow oocytes. Gene expression of developmental competence markers (ZAR1, MATER, and IGF2R) was also analyzed. The largest follicle diameters were similar between N-OV and OV groups on the day of estradiol treatment (d12) and the next day and decreased (P = 0.04) in the N-OV group thereafter. LH pulse secretion was different between groups (N-OV = 3.61 +/- 0.34 vs OV = 2.83 +/- 0.21 ng/ ml; P = 0.04). COC assessment showed that the number of recovered oocytes, BCB+ rate, IVF-grade oocytes and oocyte size was similar (P > 0.05) among groups, resembling adult cow patterns. ZAR1, MATER and IGF2R gene expression in oocytes were also similar (P > 0.05) in N-OV and OV groups. In conclusion, our results demonstrate a lower LH secretion profile in peripubertal Gir heifers prone to ovulate after induction protocol, and that oocyte quality is not affected on a short-term basis by ovulation itself. 650 $aBovino 653 $aCompetência oocitária 653 $aPuberdade 700 1 $aROSA, P. M. da S. 700 1 $aALVES, B. R. C. 700 1 $aMONTEIRO, C. A. S. 700 1 $aLEAL, G. R. 700 1 $aGUEDES, P. H. E. 700 1 $aCAMARGO, A. J. dos R. 700 1 $aSARAIVA, N. Z. 773 $tZygote$gv. 31, n. 4, p. 342-349, 2023.
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Embrapa Gado de Leite (CNPGL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agropecuária Oeste. Para informações adicionais entre em contato com cpao.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agropecuária Oeste. |
Data corrente: |
01/02/2005 |
Data da última atualização: |
01/02/2005 |
Autoria: |
FIETZ, C. R.; URCHEI, M. A.; SILVA, F. C. da. |
Afiliação: |
Embrapa Agropecuária Oeste, Caixa Postal 661 - 79804-970 - Dourados, MS; Instituto de Ciências Agrárias, Universidade Federal de Uberlândia, Caixa Postal 593 - 38400-902 - Uberlândia, MG. |
Título: |
Instalação e calibração de um lisímetro de pesagem em Dourados, MS. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
Ensaios e Ciência: Série Ciências Biológicas, Agrárias e da Saúde, Campo Grande, MS, v. 7, n. 2, p. 319-324, ago. 2003. |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Calibração; Dourados; Instalação; Mato Grosso do Sul. |
Thesagro: |
Evapotranspiração; Lisímetro. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00669naa a2200217 a 4500 001 1249630 005 2005-02-01 008 2003 bl uuuu u00u1 u #d 100 1 $aFIETZ, C. R. 245 $aInstalação e calibração de um lisímetro de pesagem em Dourados, MS. 260 $c2003 650 $aEvapotranspiração 650 $aLisímetro 653 $aBrasil 653 $aCalibração 653 $aDourados 653 $aInstalação 653 $aMato Grosso do Sul 700 1 $aURCHEI, M. A. 700 1 $aSILVA, F. C. da 773 $tEnsaios e Ciência: Série Ciências Biológicas, Agrárias e da Saúde, Campo Grande, MS$gv. 7, n. 2, p. 319-324, ago. 2003.
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