Data/hora: 20/06/2024 09:48:40

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 01/04/1998
Data da última atualização: | 18/04/2017
Autoria: | SILVEIRA, F. Q. de P.; MELO, I. S. de; FERREIRA FILHO, E. X.
Afiliação: | FABIANE QUIRINO DE PAULA SILVEIRA, UnB/DF; ITAMAR SOARES DE MELO, CNPMA; EDIVALDO XIMENES FERREIRA FILHO, UnB.
Título: | Carbohydrate-hydrolysing enzyme activity production by solid-state cultures of Trichoderma harzianum strains.
Ano de publicação: | 1997
Fonte/Imprenta: | Revista de Microbiologia, v.28, n.3 p.152-156, 1997.
Idioma: | Inglês
Conteúdo: | Screening of nine Trichoderma harzianum strains grown by solid-state fermentation on media containing wheat bran as the carbon source demonstrated that different carbohydrase activities were produced by most of the strains investigated. B-Glucosidase, B-xylosidase, pectinase and a-amylase activities were produced by all strains. a-Amylase, B-xylanase and B-glucosidase activities were the best yields obtained. B-Mannosidase, a-glucuronidase, carboxymethyl-cellulase and FPase activities were poorly produced. The best balance of enzyme activities was obtained from cultures of T.harzianum strains 4 and 8. The procedure for enzyme extraction (25 mM sodium acetate buffer, pH 5.0) of T.harzianum strain C was compared with a second method using the above buffer sol ution containing 0.1% tween 80 plus blending in a homogenizer. The results indicated that the best yield of specific activity was obtained from the former extraction procedure. Furthermore, the protein content obtained by using this extraction procedure was very low. Carboxymethyl-cellulase activity was only detected when the second extraction procedure was used.
Palavras-Chave: | Cell wall; Enzyme production; Protein determination; Solid-state cultures.
Thesaurus Nal: | culture media; electrophoresis; extraction; strains.
Categoria do assunto: | --
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (3380-1) - UPC - AP - DD -  -  -  - DIS