Data/hora: 21/06/2024 05:45:46

Biblioteca(s): | Embrapa Algodão; Embrapa Semiárido.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 17/10/2017
Data da última atualização: | 26/12/2017
Autoria: | SANTOS, J. W. M. dos; SILVA, J. F. da; FERREIRA, T. D. dos S.; DIAS, M. A. M.; FRAIZ, A. C. R.; ESCOBAR, I. E. C.; SANTOS, R. C. dos; LIMA, L. M. de; MORGANTE, C. V.; FERNANDES JUNIOR, P. I.
Afiliação: | JONNATHAN WHINY MORAES DOS SANTOS, UNIVERSIDADE ESTADUAL DA PARAIBA; JÉSSICA FERNANDA DA SILVA, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO; TAINÁ DOURADO DOS SANTOS FERREIRA, UNIVERSIDADE ESTADUAL DA BAHIA; MARCOS ANDRÉ MOURA DIAS, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO; ANA CARLA RESENDE FRAIZ, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO; INDIRA ELENA COSTA ESCOBAR, UNIVERSIDADE FEDERAL DO VALE DO SÃO FANCISCO; ROSEANE CAVALCANTI DOS SANTOS, CNPA; LIZIANE MARIA DE LIMA, CNPA; CAROLINA VIANNA MORGANTE, CPATSA; PAULO IVAN FERNANDES JUNIOR, CPATSA.
Título: | Molecular and symbiotic characterization of peanut bradyrhizobia from the semi-arid region of Brazil.
Ano de publicação: | 2017
Fonte/Imprenta: | Applied Soil Ecology, v. 121, p. 177-184, 2017.
Idioma: | Inglês
Conteúdo: | Peanut (Arachis hypogaea L.) is a legume crop native from South America and grown worldwide. This is well adapted to the unfavorable edaphoclimatic conditions found in Brazilian Semi-Arid region, where is cultivated mainly by smallholders. The selection of new rhizobial isolates is important to increase the rates of biological nitrogen fixation and yield. Furthermore, the diversity evaluation of new bacterial isolates may help us to understand the ecology of these groups in regions where little information are available. In this context, this study aimed to evaluate the phylogenetic relationships of peanut bradyrhizobia from Brazilian Semi-Arid soils by analysis of 16S rRNA, recA, nodC and nifD gene sequences, as well as their symbiotic performance. A trap-host experiment was performed using two soil samples from the municipality of Barbalha, Ceará, Brazil, taken under peanut cropping and one soil sample from the municipality of Juazeiro, Bahia, Brazil, covered by the native Arachis triseminata. Seeds of peanut cv. BR1 were sown and the plants were grown until 52 days after emergence. The nodules were inoculated in YMA medium for isolation of slow-growing bacteria. The Box-PCR fingerprinting and the 16S rRNA gene sequences was evaluated for all bacteria and recA, nodC and nifD were sequenced for the six selected isolates. Selected bacteria were evaluated according to their symbiotic characteristics in a greenhouse experiment. Ten slow-growing isolates were obtained and classified within the genus Bradyrhizobium, by the 16S rRNA sequence analysis and showed divergent Box-PCR profiles. All bacteria were clustered within the B. japonicum clade. The bacteria from Juazeiro were closely related to B. yuanmingense, and those from Barbalha showed similarity to B. yuanmingense, B. kavangense and B. guangxiense. The sequences of recA showed partial congruence to the 16S rRNA gene analysis but the phylogeny trees based on the symbiotic genes were not related, indicating horizontal gene transfer. All bacteria were able to efficiently nodulate the peanut and the isolate ESA 83, pointed out regarding the nitrogen fixation ability and were selected for further analysis in nonsterile soils and field conditions.
Palavras-Chave: | Arachis triseminata; Biological nitrogen fixation; Bradyrhizobia; FBN; Fixação biológica do nitrogênio; Inoculant; Peanut; Rizóbio.
Thesagro: | Amendoim; Arachis Hypogaea; Bactéria; Fixação simbiótica de nitrogênio; Leguminosa; Solo.
Thesaurus Nal: | Bradyrhizobium; Nitrogen fixation; Peanuts.

Categoria do assunto:

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X Pesquisa, Tecnologia e Engenharia

Registro original: | Embrapa Algodão (CNPA)

Exemplares:

CPATSA - (56845-1) - UPC - AP - DD -  -  -  - DIS
CNPA - (28420-1) - UPC - AP - DD -  -  -  - DIS