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| 241. |  | GHERI, E. de O.; CRUZ, M. C. P. da; FERREIRA, M. E.; PALMA, L. A. S. da. Nível crítico de fósforo no solo para Panicum maximum Jacq. cv. Tanzania. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 35, n. 9, p. 1809-16, set. 2000. Título em inglês: Critical level of soil phosphorus to Panicum maximum cv. Tanzânia.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 242. | | RANGEL, P. H. N.; BRONDANI, C.; ZIMMERMANN, F. J. P.; CORDEIRO, A. C. C.; FERREIRA, M. E. Obtenção de linhagens Sativa vetoras de genes de - Oryza glumaepatula. In: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 2.; REUNIÃO DA CULTURA DO ARROZ IRRIGADO, 24., 2001, Porto Alegre. Anais... Porto Alegre: IRGA, 2001. p. 18-20.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 244. | | ALBUQUERQUE, R. W.; VIEIRA, D. L. M.; VICENTE, L. E.; ARAUJO, L. S. de; FERREIRA, M. E.; GROHMANN, C. H. Comparing forest restoration canopy cover measurements using RGB and multispectral sensors onboard drones. In: SIMPÓSIO BRASILEIRO DE SENSORIAMENTO REMOTO, 20., 2023, Florianópolis. Anais [...]. São José dos Campos: Instituto Nacional de Pesquisas Espaciais, 2023. Ref. 155275. p. 53-56.| Biblioteca(s): Embrapa Meio Ambiente; Embrapa Recursos Genéticos e Biotecnologia. |
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| 245. | | RITSCHEL, P. S.; LINS, T.; TRISTAN, R. L.; BUSO, G. C. S.; BUSO, J. A.; FERREIRA, M. E. Caracterizacao de marcadores de regioes hipervariaveis do genoma e comparacao entre sistemas de marcadores baseados em PCR (RAPD e SSR) na analise genetica de acessos de melao (cucumis melo L.) In: SIMPOSIO DE RECURSOS GENETICOS PARA A AMERICA LATINA E CARIBE-SIRGEALC, 3., 2001, Londrina. Anais... Londrina: IAPAR, 2001. p.392-394| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 248. | | BRUSTOLIN, J. G.; FONTANA, V.; COMACHIO, V.; TONIETTO, J.; FERREIRA, M. E.; MAIA, J. D. G.; RITSCHEL, P. S. Caracterização genética, fenotípica e sensorial da cultivar de videira 'Moscato Branco'. In.:ENCONTRO DE INICIAÇÃO CIENTÍFICA, 13., 2015. Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2015. p. 56.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 249. | | BRUSTOLIN, J. G.; FONTANA, V.; COMACHIO, V.; TONIETTO, J.; FERREIRA, M. E.; MAIA, J. D. G.; RITSCHEL, P. S. Caracterização genética, fenotípica e sensorial da cultivar de videira 'Moscato Branco'. In.:ENCONTRO DE INICIAÇÃO CIENTÍFICA, 13., 2015. Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2015. p. 56.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 250. | | LOPES, M. A.; FALEIRO, F. G.; FERREIRA, M. E.; LOPES, D. B.; VIVIAN, R.; BOITEUX, L. S. Embrapa's contribution to the development of new plant varieties and their impact on Brazilian agriculture. Crop Breeding and Applied Biotechnology, Viçosa, MG, S2, p. 31-46, 2012.| Biblioteca(s): Embrapa Cerrados; Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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| 251. | | SILVA, N. J. M. L.; LINS, T. C. L.; AMARAL, Z.; RANGEL, P. H. N.; FERREIRA, M. E. Fingerprinting de DNA na avaliação de duplicações de genótipos no banco de germoplasma de arroz (Oryza sativa L.). In: WORKSHOP DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 4., 1999, Brasília. Anais: resumos dos trabalhos. Brasília, DF: EMBRAPA-CENARGEN, 1999. p. 83.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 252. | | TERRA, T. G. R.; RODRIGUES, L. L.; RANGEL, P. H. N.; ABREU, A. G. de; FERREIRA, M. E. Fenotipagem do sistema radicular em uma coleção nuclear de arroz de terras altas. In: SEMINÁRIO JOVENS TALENTOS, 7., 2013, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2013. p. 97. (Embrapa Arroz e Feijão. Documentos, 292). Pôster - pós-graduação.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 253. | | LEITE, J. N. F.; CRUZ, M. C. P. da; FERREIRA, M. E.; ANDRIOLI, I.; BRAOS, L. V. Frações orgânicas e inorgânicas do fósforo no solo influenciadas por plantas de cobertura e adubação nitrogenada. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 11, p. 1880-1889, nov. 2016. Título em inglês: Organic and inorganic P forms in soil as affected by cover crops and nitrogen fertilization.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 254. | | LANNA, A. C.; CARVALHO, R. N.; FERREIRA, M. E. de O.; BASSINELLO, P. Z.; CALIARI, M.; COELHO, G. R. C. Estresse oxidativo em grãos de feijão carioca recém-colhidos. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 11., 2014, Londrina. Tecnologias para a sustentabilidade da cultura do feijão: anais. Londrina: IAPAR, 2014. CONAFE| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 255. | | TERRA, T. G. R.; LEAL, T. C. A. de B.; RANGEL, P. H. N.; FERREIRA, M. E.; BARROS, H. B. Estudo de dez genótipos de arroz para tolerância à seca no cerrado sul tocantinense. In: SIMPÓSIO NACIONAL CERRADO, 9.; SIMPÓSIO INTERNACIONAL SAVANAS TROPICAIS, 2., 2008, Brasília, DF. Desafios e estratégias para o equilíbrio entre sociedade, agronegócio e recursos naturais: anais... Planaltina, DF: Embrapa Cerrados, 2008. 1 CD-ROM. Organizado por Fábio Gelape Faleiro e Austeclínio Lopes de Farias Neto. Apresentado também no:SIMPÓSIO NACIONAL CERRADO, 9; SIMPÓSIO INTERNACIONAL SAVANAS TROPICAIS, 2., 2009, Planaltina. Menções honrosas. Planaltina, DF: Embrapa Cerrados, 2009. 498 p. il. color. Editado por: Fábio Gelape Faleiro e...| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 256. | | TERRA, T. G. R.; LEAL, T. C. A. de B.; RANGEL, P. H. N.; FERREIRA, M. E.; BARROS, H. B. Estudo de dez genótipos de arroz para tolerância à seca no cerrado sul tocantinense. In: SIMPÓSIO NACIONAL CERRADO, 9.; SIMPÓSIO INTERNACIONAL SAVANAS TROPICAIS, 2., 2008, Brasília, DF. Anais... Planaltina, DF: Embrapa Cerrados, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 257. | | RITSCHEL, P. S.; REVERS, L. F.; LEITE, G. B.; FERREIRA, M. E.; OLIVEIRA, P. R. D. de. Genetic analysis of accessions in a pear germplasm bank. In: INTERNATIONAL PEAR SYMPOSIUM, 10., 2007, Peniche. Programme and abstracts. [Alcobaça, Portugal: ISHS], 2007. p. 40. Resumo PO 37.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 258. | | BUSO, G. S. C.; BOITEUX, L. S.; BIANCHETTI, L.; REIFSCHNEIDER, F. J. B.; LOPES, C. A.; FERREIRA, M. E. Germplasm diversity, disease resistance and quality trait analysis of Capsicum species using molecular tools. In: INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT, ANIMAL & MICROBE GENOMES RESEARCH, 10., 2002, San Diego. San Diego: BIOSYSTEMS, 2002. p. 70.| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 453 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
06/05/2013 |
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Data da última atualização: |
22/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - B |
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Autoria: |
RITSCHEL, P. S.; LINS, T. C. de L.; LOURENCO, R. L.; BUSO, G. S. C.; BUSO, J. A.; FERREIRA, M. E. |
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Afiliação: |
PATRICIA SILVA RITSCHEL, CNPUV; TULIO CESAR DE LIMA LINS, CPAC; ISABELA TRISTAN LOURENCO, CENARGEN; GLAUCIA SALLES CORTOPASSI BUSO, CENARGEN; JOSE AMAURI BUSO, SRI; MARCIO ELIAS FERREIRA, CENARGEN. |
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Título: |
Development of microsatellite markers from an enriched genomic library for genetic analysis of melon (Cucumis melo L.) |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
BMC Plant Biology, London, v. 4, n. 1, p. 9-24, 2004. |
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Páginas: |
16 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Despite the great advances in genomic technology observed in several crop species, the availability of molecular tools such as microsatellite markers has been limited in melon (Cucumis melo L.) and cucurbit species. The development of microsatellite markers will have a major impact on genetic analysis and breeding of melon, especially on the generation of marker saturated genetic maps and implementation of marker assisted breeding programs. Genomic microsatellite enriched libraries can be an efficient alternative for marker development in such species. Results: Seven hundred clones containing microsatellite sequences from a Tsp-AG/TC microsatellite enriched library were identified and one-hundred and forty-four primer pairs designed and synthesized. When 67 microsatellite markers were tested on a panel of melon and other cucurbit accessions, 65 revealed DNA polymorphisms among the melon accessions. For some cucurbit species, such as Cucumis sativus, up to 50% of the melon microsatellite markers could be readily used for DNA polymophism assessment, representing a significant reduction of marker development costs. A random sample of 25 microsatellite markers was extracted from the new microsatellite marker set and characterized on 40 accessions of melon, generating an allelic frequency database for the species. The average expected heterozygosity was 0.52, varying from 0.45 to 0.70, indicating that a small set of selected markers should be sufficient to solve questions egarding genotype identity and variety protection. Genetic distances based on microsatellite polymorphism were congruent with data obtained from RAPD marker analysis. Mapping analysis was initiated with 55 newly developed markers and most primers showed segregation according to Mendelian expectations. Linkage analysis detected linkage between 56% of the markers, distributed in nine linkage groups. Conclusions: Genomic library microsatellite enrichment is an efficient procedure for marker development in melon. One-hundred and forty-four new markers were developed from Tsp-AG/TC genomic library. This is the first reported attempt of successfully using enriched library for microsatellite marker development in the species. A sample of the microsatellite markers tested proved efficient for genetic analysis of melon, including genetic distance estimates and identity tests. Linkage analysis indicated that the markers developed are dispersed throughout the genome and should be very useful for genetic analysis of melon. MenosBackground: Despite the great advances in genomic technology observed in several crop species, the availability of molecular tools such as microsatellite markers has been limited in melon (Cucumis melo L.) and cucurbit species. The development of microsatellite markers will have a major impact on genetic analysis and breeding of melon, especially on the generation of marker saturated genetic maps and implementation of marker assisted breeding programs. Genomic microsatellite enriched libraries can be an efficient alternative for marker development in such species. Results: Seven hundred clones containing microsatellite sequences from a Tsp-AG/TC microsatellite enriched library were identified and one-hundred and forty-four primer pairs designed and synthesized. When 67 microsatellite markers were tested on a panel of melon and other cucurbit accessions, 65 revealed DNA polymorphisms among the melon accessions. For some cucurbit species, such as Cucumis sativus, up to 50% of the melon microsatellite markers could be readily used for DNA polymophism assessment, representing a significant reduction of marker development costs. A random sample of 25 microsatellite markers was extracted from the new microsatellite marker set and characterized on 40 accessions of melon, generating an allelic frequency database for the species. The average expected heterozygosity was 0.52, varying from 0.45 to 0.70, indicating that a small set of selected markers should be sufficient to solve quest... Mostrar Tudo |
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Palavras-Chave: |
Análise genética; Marcador microsatélite; Melhoramento genético. |
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Thesagro: |
Genética; Melão. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/82420/1/RITSCHEL-BMCPlantBiol-v4p1-2004.pdf
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|
Marc: |
LEADER 03292naa a2200253 a 4500
001 1957237
005 2016-02-22
008 2004 bl uuuu u00u1 u #d
100 1 $aRITSCHEL, P. S.
245 $aDevelopment of microsatellite markers from an enriched genomic library for genetic analysis of melon (Cucumis melo L.)$h[electronic resource]
260 $c2004
300 $a16 p.
520 $aBackground: Despite the great advances in genomic technology observed in several crop species, the availability of molecular tools such as microsatellite markers has been limited in melon (Cucumis melo L.) and cucurbit species. The development of microsatellite markers will have a major impact on genetic analysis and breeding of melon, especially on the generation of marker saturated genetic maps and implementation of marker assisted breeding programs. Genomic microsatellite enriched libraries can be an efficient alternative for marker development in such species. Results: Seven hundred clones containing microsatellite sequences from a Tsp-AG/TC microsatellite enriched library were identified and one-hundred and forty-four primer pairs designed and synthesized. When 67 microsatellite markers were tested on a panel of melon and other cucurbit accessions, 65 revealed DNA polymorphisms among the melon accessions. For some cucurbit species, such as Cucumis sativus, up to 50% of the melon microsatellite markers could be readily used for DNA polymophism assessment, representing a significant reduction of marker development costs. A random sample of 25 microsatellite markers was extracted from the new microsatellite marker set and characterized on 40 accessions of melon, generating an allelic frequency database for the species. The average expected heterozygosity was 0.52, varying from 0.45 to 0.70, indicating that a small set of selected markers should be sufficient to solve questions egarding genotype identity and variety protection. Genetic distances based on microsatellite polymorphism were congruent with data obtained from RAPD marker analysis. Mapping analysis was initiated with 55 newly developed markers and most primers showed segregation according to Mendelian expectations. Linkage analysis detected linkage between 56% of the markers, distributed in nine linkage groups. Conclusions: Genomic library microsatellite enrichment is an efficient procedure for marker development in melon. One-hundred and forty-four new markers were developed from Tsp-AG/TC genomic library. This is the first reported attempt of successfully using enriched library for microsatellite marker development in the species. A sample of the microsatellite markers tested proved efficient for genetic analysis of melon, including genetic distance estimates and identity tests. Linkage analysis indicated that the markers developed are dispersed throughout the genome and should be very useful for genetic analysis of melon.
650 $aGenética
650 $aMelão
653 $aAnálise genética
653 $aMarcador microsatélite
653 $aMelhoramento genético
700 1 $aLINS, T. C. de L.
700 1 $aLOURENCO, R. L.
700 1 $aBUSO, G. S. C.
700 1 $aBUSO, J. A.
700 1 $aFERREIRA, M. E.
773 $tBMC Plant Biology, London$gv. 4, n. 1, p. 9-24, 2004.
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