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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/02/2008 |
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Data da última atualização: |
12/02/2008 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRASILEIRO-VIDAL, A. C.; SANTOS-SEREJO, J. A. dos; SOARES FILHO, W. dos S.; GUERRA, M. |
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Afiliação: |
A. C. Brasileiro-Vidal, UFPE; Janay Almeida dos Santos-Serejo, CNPMF; Walter dos Santos Soares Filho, CNPMF; M. Guerra, UFPE. |
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Título: |
A simple chromosomal marker can reliably distinguishes Poncirus from Citrus species. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Genetica, Netherlands, v. 129, n. 3, p. 273-279, mar. 2007. |
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Idioma: |
Português |
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Notas: |
DOI 10.1007/s10709-006-0007-4 |
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Conteúdo: |
Several chromosome types have been recognized in Citrus and related genera by chromomycin A3 (CMA) banding patterns and fluorescent in situ hybridization (FISH). They can be used to characterize cultivars and species or as markers in hybridization and backcrossing experiments. In the present work, characterization of six cultivars of P. trifoliata ("Barnes", "Fawcett", "Flying Dragon", "Pomeroy", "Rubidoux", "USDA") and one P. trifoliata × C. limonia hybrid was performed by sequential analyses of CMA banding and FISH using 5S and 45S rDNA as probes. All six cultivars showed a similar CMA+ banding pattern with the karyotype formula 4B + 8D + 6F. The capital letters indicate chromosomal types: B, a chromosome with one telomeric and one proximal band; D, with only one telomeric band; F, without bands. In situ hybridization labeling was also similar among cultivars. Three chromosome pairs displayed a closely linked set of 5S and 45S rDNA sites, two of them co-located with the proximal band of the B type chromosomes (B/5S-45S) and the third one co-located with the terminal band of a D pair (D/5S-45S). The B/5S-45S chromosome has never been found in any citrus accessions investigated so far. Therefore, this B chromosome can be used as a marker to recognize the intergeneric Poncirus × Citrus hybrids. The intergeneric hybrid analyzed here displayed the karyotype formula 4B + 8D + 6F, with two chromosome types B/5S-45S and two D/5S-45S. The karyotype formula and the presence of two B/5S-45S chromosomes clearly indicate that the plant investigated is a symmetric hybrid. It also demonstrates the suitability of karyotype analyses to differentiate zygotic embryos or somatic cell fusions involving trifoliate orange germplasm. MenosSeveral chromosome types have been recognized in Citrus and related genera by chromomycin A3 (CMA) banding patterns and fluorescent in situ hybridization (FISH). They can be used to characterize cultivars and species or as markers in hybridization and backcrossing experiments. In the present work, characterization of six cultivars of P. trifoliata ("Barnes", "Fawcett", "Flying Dragon", "Pomeroy", "Rubidoux", "USDA") and one P. trifoliata × C. limonia hybrid was performed by sequential analyses of CMA banding and FISH using 5S and 45S rDNA as probes. All six cultivars showed a similar CMA+ banding pattern with the karyotype formula 4B + 8D + 6F. The capital letters indicate chromosomal types: B, a chromosome with one telomeric and one proximal band; D, with only one telomeric band; F, without bands. In situ hybridization labeling was also similar among cultivars. Three chromosome pairs displayed a closely linked set of 5S and 45S rDNA sites, two of them co-located with the proximal band of the B type chromosomes (B/5S-45S) and the third one co-located with the terminal band of a D pair (D/5S-45S). The B/5S-45S chromosome has never been found in any citrus accessions investigated so far. Therefore, this B chromosome can be used as a marker to recognize the intergeneric Poncirus × Citrus hybrids. The intergeneric hybrid analyzed here displayed the karyotype formula 4B + 8D + 6F, with two chromosome types B/5S-45S and two D/5S-45S. The karyotype formula and the presence of two B... Mostrar Tudo |
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Palavras-Chave: |
Chromosome; CMA; rDNA. |
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Thesaurus Nal: |
Citrus; fish; Poncirus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02411naa a2200241 a 4500
001 1654619
005 2008-02-12
008 2007 bl --- 0-- u #d
100 1 $aBRASILEIRO-VIDAL, A. C.
245 $aA simple chromosomal marker can reliably distinguishes Poncirus from Citrus species.
260 $c2007
500 $aDOI 10.1007/s10709-006-0007-4
520 $aSeveral chromosome types have been recognized in Citrus and related genera by chromomycin A3 (CMA) banding patterns and fluorescent in situ hybridization (FISH). They can be used to characterize cultivars and species or as markers in hybridization and backcrossing experiments. In the present work, characterization of six cultivars of P. trifoliata ("Barnes", "Fawcett", "Flying Dragon", "Pomeroy", "Rubidoux", "USDA") and one P. trifoliata × C. limonia hybrid was performed by sequential analyses of CMA banding and FISH using 5S and 45S rDNA as probes. All six cultivars showed a similar CMA+ banding pattern with the karyotype formula 4B + 8D + 6F. The capital letters indicate chromosomal types: B, a chromosome with one telomeric and one proximal band; D, with only one telomeric band; F, without bands. In situ hybridization labeling was also similar among cultivars. Three chromosome pairs displayed a closely linked set of 5S and 45S rDNA sites, two of them co-located with the proximal band of the B type chromosomes (B/5S-45S) and the third one co-located with the terminal band of a D pair (D/5S-45S). The B/5S-45S chromosome has never been found in any citrus accessions investigated so far. Therefore, this B chromosome can be used as a marker to recognize the intergeneric Poncirus × Citrus hybrids. The intergeneric hybrid analyzed here displayed the karyotype formula 4B + 8D + 6F, with two chromosome types B/5S-45S and two D/5S-45S. The karyotype formula and the presence of two B/5S-45S chromosomes clearly indicate that the plant investigated is a symmetric hybrid. It also demonstrates the suitability of karyotype analyses to differentiate zygotic embryos or somatic cell fusions involving trifoliate orange germplasm.
650 $aCitrus
650 $afish
650 $aPoncirus
653 $aChromosome
653 $aCMA
653 $arDNA
700 1 $aSANTOS-SEREJO, J. A. dos
700 1 $aSOARES FILHO, W. dos S.
700 1 $aGUERRA, M.
773 $tGenetica, Netherlands$gv. 129, n. 3, p. 273-279, mar. 2007.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Trigo. |
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Data corrente: |
14/05/1997 |
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Data da última atualização: |
10/06/2008 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Circulação/Nível: |
-- - -- |
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Autoria: |
LUZ, W. C. da; MENDES, M. A. S.; FERREIRA, M. A. S. V.; URBEN, A. F. |
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Afiliação: |
EMBRAPA-CENARGEN. |
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Título: |
Tilletia indica em trigo no sul do Rio Grande do Sul e medidas para erradicacao. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Fitopatologia Brasileira, Brasília, DF, v. 18, p. 329, ago. 1993. |
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Idioma: |
Português |
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Notas: |
Edição dos Resumos do XXVI Congresso Brasileiro de Fitopatologia, Aracajú, ago. 1993. |
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Palavras-Chave: |
Eradication; Rio Grande do Sul. |
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Thesagro: |
Erradicação; Tilletia Indica; Trigo. |
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Thesaurus NAL: |
wheat. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00726naa a2200229 a 4500
001 1170950
005 2008-06-10
008 1993 bl uuuu u00u1 u #d
100 1 $aLUZ, W. C. da
245 $aTilletia indica em trigo no sul do Rio Grande do Sul e medidas para erradicacao.
260 $c1993
500 $aEdição dos Resumos do XXVI Congresso Brasileiro de Fitopatologia, Aracajú, ago. 1993.
650 $awheat
650 $aErradicação
650 $aTilletia Indica
650 $aTrigo
653 $aEradication
653 $aRio Grande do Sul
700 1 $aMENDES, M. A. S.
700 1 $aFERREIRA, M. A. S. V.
700 1 $aURBEN, A. F.
773 $tFitopatologia Brasileira, Brasília, DF$gv. 18, p. 329, ago. 1993.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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