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| 14. |  | FERREIRA, J. R.; CONSOLI, L.; TORRES, G. A. M. Construção de mapa genético para estudos de QTL de resistência de trigo a Magnaporthe oryzae. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 15.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 12., 2020, Passo Fundo. Resumos... Brasília, DF: Embrapa, p. 51, 2021.| Biblioteca(s): Embrapa Trigo. |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
28/08/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FERREIRA, J. R.; MINELLA, E.; DELATORRE, C. A.; DELHAIZE, E.; RYAN, P. R.; PEREIRA, J. F. |
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Afiliação: |
Jéssica Rosset Ferreira, UFRGS; EUCLYDES MINELLA, CNPT; Carla Andréa Delatorre, UFRGS; Emmanuel Delhaize, CSIRO; Peter R. Ryan, CSIRO; JORGE FERNANDO PEREIRA, CNPGL. |
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Título: |
Conventional and transgenic strategies to enhance the acid soil tolerance of barley. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Breeding, v. 38, n. 12, 2018. |
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Páginas: |
11 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The aluminum (Al3+) tolerance of barley cultivars predominately from Brazil was compared to that of cultivars from other countries, wild barley accessions, and a transgenic line (L5) over-expressing TaALMT1, the major Al3+ tolerance gene from wheat. After screening conventional germplasm for Al3+ tolerance in hydroponics, 18 genotypes were further characterized in a short-term soil experiment. Among the Brazilian cultivars, Antarctica 01 and BRS Mariana showed the greatest relative root length (RRL) in acid soil. However, these cultivars were significantly less tolerant than the foreign cultivars Dayton (USA) and Murasakimochi (Japan) and the transgenic line L5 which out-performed all conventional genotypes. In long-term growth trials, the transgenic line produced the greatest relative root and relative shoot dry weight. Relative grain yield was greatest in the transgenic line and Dayton. All genotypes were also scored for two genetic markers linked to HvAACT1, the major Al3+ tolerance gene in barley. One marker detects a 1-kb insertion in the promoter that increases gene expression and leads to increased Al3+- activated citrate efflux from root apices. The other marker detects a 21-bp indel downstream of the coding region. The 1-kb insertion was only detected in Dayton and Murasakimochi that were the best performing cultivars among the non-transgenic germplasm. Interestingly, the Brazilian cultivars with an intermediate level of tolerance, Antarctica 01 and BRS Mariana, lacked the 1-kb insertion but had enhanced HvAACT1 expression compared to an Al3+-sensitive cultivar. No clear correlation was observed between Al3+ tolerance and the 21-bp indel marker in the short-term soil trials. We conclude that improved Al3+ tolerance in barley could be achieved by combining the best allele of HvAACT1 along with TaALMT1 as a transgene. MenosAbstract The aluminum (Al3+) tolerance of barley cultivars predominately from Brazil was compared to that of cultivars from other countries, wild barley accessions, and a transgenic line (L5) over-expressing TaALMT1, the major Al3+ tolerance gene from wheat. After screening conventional germplasm for Al3+ tolerance in hydroponics, 18 genotypes were further characterized in a short-term soil experiment. Among the Brazilian cultivars, Antarctica 01 and BRS Mariana showed the greatest relative root length (RRL) in acid soil. However, these cultivars were significantly less tolerant than the foreign cultivars Dayton (USA) and Murasakimochi (Japan) and the transgenic line L5 which out-performed all conventional genotypes. In long-term growth trials, the transgenic line produced the greatest relative root and relative shoot dry weight. Relative grain yield was greatest in the transgenic line and Dayton. All genotypes were also scored for two genetic markers linked to HvAACT1, the major Al3+ tolerance gene in barley. One marker detects a 1-kb insertion in the promoter that increases gene expression and leads to increased Al3+- activated citrate efflux from root apices. The other marker detects a 21-bp indel downstream of the coding region. The 1-kb insertion was only detected in Dayton and Murasakimochi that were the best performing cultivars among the non-transgenic germplasm. Interestingly, the Brazilian cultivars with an intermediate level of tolerance, Antarctica 01 and BRS Mar... Mostrar Tudo |
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Palavras-Chave: |
Aluminum tolerance; Citrate transporter; HvAACT1; Malatetransporter; TaALMT1. |
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Thesaurus NAL: |
Hydroponics. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02589naa a2200265 a 4500
001 2094656
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
100 1 $aFERREIRA, J. R.
245 $aConventional and transgenic strategies to enhance the acid soil tolerance of barley.$h[electronic resource]
260 $c2018
300 $a11 p.
520 $aAbstract The aluminum (Al3+) tolerance of barley cultivars predominately from Brazil was compared to that of cultivars from other countries, wild barley accessions, and a transgenic line (L5) over-expressing TaALMT1, the major Al3+ tolerance gene from wheat. After screening conventional germplasm for Al3+ tolerance in hydroponics, 18 genotypes were further characterized in a short-term soil experiment. Among the Brazilian cultivars, Antarctica 01 and BRS Mariana showed the greatest relative root length (RRL) in acid soil. However, these cultivars were significantly less tolerant than the foreign cultivars Dayton (USA) and Murasakimochi (Japan) and the transgenic line L5 which out-performed all conventional genotypes. In long-term growth trials, the transgenic line produced the greatest relative root and relative shoot dry weight. Relative grain yield was greatest in the transgenic line and Dayton. All genotypes were also scored for two genetic markers linked to HvAACT1, the major Al3+ tolerance gene in barley. One marker detects a 1-kb insertion in the promoter that increases gene expression and leads to increased Al3+- activated citrate efflux from root apices. The other marker detects a 21-bp indel downstream of the coding region. The 1-kb insertion was only detected in Dayton and Murasakimochi that were the best performing cultivars among the non-transgenic germplasm. Interestingly, the Brazilian cultivars with an intermediate level of tolerance, Antarctica 01 and BRS Mariana, lacked the 1-kb insertion but had enhanced HvAACT1 expression compared to an Al3+-sensitive cultivar. No clear correlation was observed between Al3+ tolerance and the 21-bp indel marker in the short-term soil trials. We conclude that improved Al3+ tolerance in barley could be achieved by combining the best allele of HvAACT1 along with TaALMT1 as a transgene.
650 $aHydroponics
653 $aAluminum tolerance
653 $aCitrate transporter
653 $aHvAACT1
653 $aMalatetransporter
653 $aTaALMT1
700 1 $aMINELLA, E.
700 1 $aDELATORRE, C. A.
700 1 $aDELHAIZE, E.
700 1 $aRYAN, P. R.
700 1 $aPEREIRA, J. F.
773 $tMolecular Breeding$gv. 38, n. 12, 2018.
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