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| 6. |  | REZENDE, J. L. P.; LOPES, H. V. S.; PAULA JUNIOR, G. G.; NEVES, A. R. Efeito da taxa real de desconto sobre a idade otima de corte de povoamentos de eucalipto, no Estado de Minas Gerais. Revista Arvore, Vicosa, v.17, n.3, p.339-350, set./dez. 1993.| Biblioteca(s): Embrapa Florestas. |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
18/12/2023 |
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Data da última atualização: |
18/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
SANTOS, T. D. dos; ASOBIA, P. C.; FERREIRA, E. P. de B. |
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Afiliação: |
THÁLYTA DUARTE DOS SANTOS, UFG; PRINCEWILL CHUKWUMA ASOBIA, UFG; ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF. |
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Título: |
Polyphasic characterization of rhizobial isolates obtained from different common bean-producing regions. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Bioscience Journal, v. 39, e39082, 2023. |
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ISSN: |
1981-3163 |
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DOI: |
https://doi.org/10.14393/BJ-v39n0a2023-67229 |
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Idioma: |
Inglês |
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Conteúdo: |
In Brazil, the common bean is a crop with significant social and economic importance. The prospecting of N2 fixing bacteria is crucial since biological nitrogen fixation (BNF) is an eco-friendly technique. This work aimed to obtain and characterize rhizobium isolates based on morpho-physiological, molecular, and symbiotic efficiency parameters, using the strains SEMIA 4077, SEMIA 4080, and SEMIA 4088 as references. The characteristics of the isolates and colonies, their tolerance to salinity and temperature, as well as their utilization of carbon sources, served as the basis for the morpho-physiological characterization. BOX-PCR, REP-PCR, and ERIC-PCR markers were used for genotypic characterization. Assessment of the symbiotic efficiency was carried out in a greenhouse, determining the number of nodules (NN), nodule dry weight (NDW), shoot dry weight (SDW), and total-N (Total-N) accumulation in the shoot. Among the isolates, those exhibiting: neutral culture medium pH, fast growth, colony diameter <2 mm, opaque transparency, homogeneous appearance, and cream color were predominant. Compared to temperature, salinity was the most restrictive factor to the growth of the isolates. Most of the isolates grew on sucrose (88.43%) and mannitol (87.28%). Genotypic analysis revealed that 90% of the isolates clustered in the same group as the reference strain SEMIA 4080. The TaMsG2R1 and BaDeG4R2 isolates showed higher Total-N in the shoot than the reference strains and should be evaluated in future studies under field conditions. MenosIn Brazil, the common bean is a crop with significant social and economic importance. The prospecting of N2 fixing bacteria is crucial since biological nitrogen fixation (BNF) is an eco-friendly technique. This work aimed to obtain and characterize rhizobium isolates based on morpho-physiological, molecular, and symbiotic efficiency parameters, using the strains SEMIA 4077, SEMIA 4080, and SEMIA 4088 as references. The characteristics of the isolates and colonies, their tolerance to salinity and temperature, as well as their utilization of carbon sources, served as the basis for the morpho-physiological characterization. BOX-PCR, REP-PCR, and ERIC-PCR markers were used for genotypic characterization. Assessment of the symbiotic efficiency was carried out in a greenhouse, determining the number of nodules (NN), nodule dry weight (NDW), shoot dry weight (SDW), and total-N (Total-N) accumulation in the shoot. Among the isolates, those exhibiting: neutral culture medium pH, fast growth, colony diameter <2 mm, opaque transparency, homogeneous appearance, and cream color were predominant. Compared to temperature, salinity was the most restrictive factor to the growth of the isolates. Most of the isolates grew on sucrose (88.43%) and mannitol (87.28%). Genotypic analysis revealed that 90% of the isolates clustered in the same group as the reference strain SEMIA 4080. The TaMsG2R1 and BaDeG4R2 isolates showed higher Total-N in the shoot than the reference strains and should be evalu... Mostrar Tudo |
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Palavras-Chave: |
Rhizobial isolates. |
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Thesagro: |
Feijão; Fixação de Nitrogênio; Phaseolus Vulgaris; Rhizobium. |
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Thesaurus NAL: |
Beans; Nitrogen fixation. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/259824/1/bj-2023-2.pdf
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Marc: |
LEADER 02319naa a2200253 a 4500
001 2159872
005 2023-12-18
008 2023 bl uuuu u00u1 u #d
022 $a1981-3163
024 7 $ahttps://doi.org/10.14393/BJ-v39n0a2023-67229$2DOI
100 1 $aSANTOS, T. D. dos
245 $aPolyphasic characterization of rhizobial isolates obtained from different common bean-producing regions.$h[electronic resource]
260 $c2023
520 $aIn Brazil, the common bean is a crop with significant social and economic importance. The prospecting of N2 fixing bacteria is crucial since biological nitrogen fixation (BNF) is an eco-friendly technique. This work aimed to obtain and characterize rhizobium isolates based on morpho-physiological, molecular, and symbiotic efficiency parameters, using the strains SEMIA 4077, SEMIA 4080, and SEMIA 4088 as references. The characteristics of the isolates and colonies, their tolerance to salinity and temperature, as well as their utilization of carbon sources, served as the basis for the morpho-physiological characterization. BOX-PCR, REP-PCR, and ERIC-PCR markers were used for genotypic characterization. Assessment of the symbiotic efficiency was carried out in a greenhouse, determining the number of nodules (NN), nodule dry weight (NDW), shoot dry weight (SDW), and total-N (Total-N) accumulation in the shoot. Among the isolates, those exhibiting: neutral culture medium pH, fast growth, colony diameter <2 mm, opaque transparency, homogeneous appearance, and cream color were predominant. Compared to temperature, salinity was the most restrictive factor to the growth of the isolates. Most of the isolates grew on sucrose (88.43%) and mannitol (87.28%). Genotypic analysis revealed that 90% of the isolates clustered in the same group as the reference strain SEMIA 4080. The TaMsG2R1 and BaDeG4R2 isolates showed higher Total-N in the shoot than the reference strains and should be evaluated in future studies under field conditions.
650 $aBeans
650 $aNitrogen fixation
650 $aFeijão
650 $aFixação de Nitrogênio
650 $aPhaseolus Vulgaris
650 $aRhizobium
653 $aRhizobial isolates
700 1 $aASOBIA, P. C.
700 1 $aFERREIRA, E. P. de B.
773 $tBioscience Journal$gv. 39, e39082, 2023.
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Embrapa Arroz e Feijão (CNPAF) |
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