Data/hora: 23/06/2024 04:20:19

Biblioteca(s): | Embrapa Pecuária Sudeste.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | B - 1
Data corrente: | 30/07/2009
Data da última atualização: | 14/03/2023
Autoria: | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M.
Afiliação: | F. PERECIN, FCAV/UNESP, Jaboticabal, SP.; SIMONE CRISTINA MEO NICIURA, CPPSE; W. YAMAZAKI, UNESP; C. R. FERREIRA, FZEA/USP, Pirassununga, SP.; G. K. F. MERIGHE, FZEA/USP, Pirassununga, SP.; F. V. MEIRELLES, FZEA/USP, Pirassununga, SP.; J. M. GARCIA, FCAV/UNESP, Jaboticabal, SP.
Título: | Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes.
Ano de publicação: | 2009
Fonte/Imprenta: | Genetics and Molecular Research, v. 8, n. 1, p. 76-85, 2009.
DOI: | 10.4238/vol8-1gmr541
Idioma: | Inglês
Conteúdo: | Cloning by nuclear transfer is often associated with poor results due to abnormal nuclear reprogramming of somatic donor cells and altered gene expression patterns. We investigated the expression patterns of imprinted genes IGF2 and IGF2R in 33- to 36-day bovine embryos and chorio-allantoic membranes derived from in vivo- and in vitro-produced embryos by somatic cell nuclear transfer (SCNT), parthenogenetic activation, and in vitro fertilization (IVF). There was a lower IGF2 expression rate in the SCNT (0.19) and parthenogenetic (0.02) groups when compared to in vivo and IVF embryos (2.01; P < 0.05). In the chorio-allantoic membranes, IGF2 showed a baseline expression pattern (P < 0.05) in parthenotes (0.001) when compared to in vivo, IVF (3.13), and SCNT (0.98) groups. IGF2R was less expressed (P < 0.05) in SCNT chorio-allantoic membranes (0.25) when compared to the in vivo group. The low expression of IGF2 in parthenogenetic embryos and chorio-allantoic membranes confirms its imprinted status in cattle. Alterations in the relative frequency of IGF2 and IGF2R transcripts were observed in SCNT-derived bovine embryos and chorio-allantoic membranes, respectively, supporting the hypothesis that abnormalities in the expression of imprinted genes are causes of the low efficiency of SCNT procedures in this species.
Palavras-Chave: | IGF2; IGF2R; Nuclear transfer.
Thesaurus Nal: | cattle; epigenetics; genomic imprinting.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/CPPSE-2009-09/18524/1/PROCISCMN2009.00069.pdf
Registro original: | Embrapa Pecuária Sudeste (CPPSE)
Exemplares: | CPPSE - (18524-1) - UPC - AP - DD - PROCI-2009.00069 - NIC - 2009.00069 - DIS