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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
16/07/2021 |
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Data da última atualização: |
16/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, A. V. de; CARVALHO, J. R. de S.; COSTA, E. S. S.; SOUZA, C. S. de; SILVA, H. F. de J.; LUZ, J. M. Q.; MACIEL, G. M.; SIQUIEROLI, A. C. S. |
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Afiliação: |
ANA VALERIA VIEIRA DE SOUZA, CPATSA; JACKSON RAFAEL DE SÁ CARVALHO; EVELYN SOPHIA SILVA COSTA; CRISTINA SOARES DE SOUZA; HERICK FERNANDO DE JESUS SILVA; JOSÉ MAGNO QUEIROZ LUZ; GABRIEL MASCARENHAS MACIEL; ANA CAROLINA SILVA SIQUIEROLI. |
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Título: |
Genetic diversity in Amburana (Amburana cearensis) accessions: hierarchical and optimization methods. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Revista Árvore, v. 45, e4508, 2021. |
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DOI: |
https://doi.org/10.1590/1806-908820210000008 |
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Idioma: |
Inglês |
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Conteúdo: |
The evaluation of accessions in a germplasm bank is essential for determining the potential parents in conservation programs, especially for native trees. This study aimed to determine the genetic diversity among 68 Amburana cearensis genotypes from different locations in the state of Pernambuco, Brazil. Their genetic patterns were evaluated by Inter Simple Sequence Repeat (ISSR) molecular markers and genetic divergence was evaluated through multivariate analyses using different clustering methods. The optimization method used (Tocher) was in agreement with all the hierarchical models used, in which clustering of the genotypes occurred similarly, specifically for the accession BB116, which is an important genetic material to be preserved and studied. Among the various hierarchical methods applied, the Average Linkage method exhibited higher discrimination power, allowing identification of a larger number of divergent groups, thus implying wide genetic diversity among A. cearensis accessions. |
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Palavras-Chave: |
Amburana cearensis; Amburana de cheiro; Árvore nativa; Conservação genética; Planta nativa. |
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Thesagro: |
Banco de Germoplasma; Caatinga; Genótipo; Imburana de Cambão; Planta Medicinal. |
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Thesaurus Nal: |
Natural resources. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/224493/1/GENETIC-DIVERSITY-IN-AMBURANA-Amburana-cearensis-2021.pdf
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Marc: |
LEADER 02054naa a2200349 a 4500
001 2132967
005 2021-07-16
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/1806-908820210000008$2DOI
100 1 $aSOUZA, A. V. de
245 $aGenetic diversity in Amburana (Amburana cearensis) accessions$bhierarchical and optimization methods.$h[electronic resource]
260 $c2021
520 $aThe evaluation of accessions in a germplasm bank is essential for determining the potential parents in conservation programs, especially for native trees. This study aimed to determine the genetic diversity among 68 Amburana cearensis genotypes from different locations in the state of Pernambuco, Brazil. Their genetic patterns were evaluated by Inter Simple Sequence Repeat (ISSR) molecular markers and genetic divergence was evaluated through multivariate analyses using different clustering methods. The optimization method used (Tocher) was in agreement with all the hierarchical models used, in which clustering of the genotypes occurred similarly, specifically for the accession BB116, which is an important genetic material to be preserved and studied. Among the various hierarchical methods applied, the Average Linkage method exhibited higher discrimination power, allowing identification of a larger number of divergent groups, thus implying wide genetic diversity among A. cearensis accessions.
650 $aNatural resources
650 $aBanco de Germoplasma
650 $aCaatinga
650 $aGenótipo
650 $aImburana de Cambão
650 $aPlanta Medicinal
653 $aAmburana cearensis
653 $aAmburana de cheiro
653 $aÁrvore nativa
653 $aConservação genética
653 $aPlanta nativa
700 1 $aCARVALHO, J. R. de S.
700 1 $aCOSTA, E. S. S.
700 1 $aSOUZA, C. S. de
700 1 $aSILVA, H. F. de J.
700 1 $aLUZ, J. M. Q.
700 1 $aMACIEL, G. M.
700 1 $aSIQUIEROLI, A. C. S.
773 $tRevista Árvore$gv. 45, e4508, 2021.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
21/12/2022 |
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Data da última atualização: |
21/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, A. N. da; ALVES, L.; OSOWSKI, G. V.; SABEI, L.; FERRAZ, P. A.; PUGLIESI, G.; MARQUES, M. G.; ZANELLA, R.; ZANELLA, A. J. |
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Afiliação: |
ARTHUR NERY DA SILVA, Universidade de São Paulo; LUANA ALVES, Universidade de São Paulo; GERMANA VIZZOTTO OSOWSKI, Universidade de São Paulo; LEANDRO SABEI, Universidade de São Paulo; PRISCILA ASSIS FERRAZ, Universidade de São Paulo; GUILHERME PUGLIESI, Universidade de São Paulo; MARIANA GROKE MARQUES, CNPSA; RICARDO ZANELLA, Universidade de Passo Fundo; ADROALDO JOSÉ ZANELLA, Universidade de São Paulo. |
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Título: |
Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Genes, v. 13, n. 5, p. 1-13, 2022. |
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DOI: |
https://doi.org/10.3390/genes13050769 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The corpus luteum (CL) is a temporary endocrine gland that plays a decisive role in the reproductive physiology of gilts. Recently, it has been suggested that exogenous factors may compromise the normal functioning of the CL. In the present study, we aimed to understand to what extent an acute and systemic challenge with lipopolysaccharide (LPS) on the day of estrus could compromise gene expression of gilts' CLs housed in different welfare conditions. For this, we housed 42 gilts in three different housing systems: crates, indoor group pens, and outdoor housing. Then, we challenged six females from each group with LPS and eight with saline (SAL) on the day of estrus. After slaughtering the gilts on the fifth day after the challenge, ovaries were collected for gene expression analysis by RT-qPCR. Housing system and LPS challenge did not have a significant interaction for any genes evaluated; thus, their effects were studied separately. We identified significant (p < 0.05) downregulation of the angiogenic genes VEGF and FTL1 among LPS-challenged animals. Meanwhile, we also observed upregulation of HSD3B1 gene among LPS-challenged animals. We found that STAR and LHCGR genes were differentially expressed depending on the housing system, which indicates that the environment may affect adaptation capabilities. Our results indicate that an acute health challenge on the estrus day alters CL gene expression; however, the role of the housing system remains uncertain. |
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Palavras-Chave: |
Acute stress; Angiogênese; Estresse agudo; Expressão gênica; LPS; Marrãs; RT-qPCR. |
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Thesagro: |
Marra; Reprodução Animal. |
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Thesaurus NAL: |
Angiogenesis; Animal development; Gilts; Swine. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150143/1/10086.pdf
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Marc: |
LEADER 02566naa a2200385 a 4500
001 2150143
005 2022-12-21
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/genes13050769$2DOI
100 1 $aSILVA, A. N. da
245 $aHousing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts.$h[electronic resource]
260 $c2022
520 $aAbstract: The corpus luteum (CL) is a temporary endocrine gland that plays a decisive role in the reproductive physiology of gilts. Recently, it has been suggested that exogenous factors may compromise the normal functioning of the CL. In the present study, we aimed to understand to what extent an acute and systemic challenge with lipopolysaccharide (LPS) on the day of estrus could compromise gene expression of gilts' CLs housed in different welfare conditions. For this, we housed 42 gilts in three different housing systems: crates, indoor group pens, and outdoor housing. Then, we challenged six females from each group with LPS and eight with saline (SAL) on the day of estrus. After slaughtering the gilts on the fifth day after the challenge, ovaries were collected for gene expression analysis by RT-qPCR. Housing system and LPS challenge did not have a significant interaction for any genes evaluated; thus, their effects were studied separately. We identified significant (p < 0.05) downregulation of the angiogenic genes VEGF and FTL1 among LPS-challenged animals. Meanwhile, we also observed upregulation of HSD3B1 gene among LPS-challenged animals. We found that STAR and LHCGR genes were differentially expressed depending on the housing system, which indicates that the environment may affect adaptation capabilities. Our results indicate that an acute health challenge on the estrus day alters CL gene expression; however, the role of the housing system remains uncertain.
650 $aAngiogenesis
650 $aAnimal development
650 $aGilts
650 $aSwine
650 $aMarra
650 $aReprodução Animal
653 $aAcute stress
653 $aAngiogênese
653 $aEstresse agudo
653 $aExpressão gênica
653 $aLPS
653 $aMarrãs
653 $aRT-qPCR
700 1 $aALVES, L.
700 1 $aOSOWSKI, G. V.
700 1 $aSABEI, L.
700 1 $aFERRAZ, P. A.
700 1 $aPUGLIESI, G.
700 1 $aMARQUES, M. G.
700 1 $aZANELLA, R.
700 1 $aZANELLA, A. J.
773 $tGenes$gv. 13, n. 5, p. 1-13, 2022.
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Embrapa Suínos e Aves (CNPSA) |
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