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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
13/09/2021 |
Data da última atualização: |
15/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RIBEIRO, T. P.; LOURENCO, I. T.; MELO, B. P. de; MORGANTE, C. V.; SALLES FILHO, A.; LINS, C. B. J.; FERREIRA, G. F.; MELLO, G. N.; MACEDO, L. L. P. de; LUCENA, W. A.; SILVA, M. C. M. da; OLIVEIRA‑NETO, O. B.; SA, M. F. G. de. |
Afiliação: |
THUANNE PIRES RIBEIRO, UNB; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; BRUNO PAES DE MELO, UFV; CAROLINA VIANNA MORGANTE, CPATSA; ALVARO SALLES FILHO, UCB; CAMILA BARROZO JESUS LINS; GILANNA FALCÃO FERREIRA; GLÊNIA NUNES MELLO; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; WAGNER ALEXANDRE LUCENA, Cenargen; MARIA CRISTINA MATTAR DA SILVA, Cenargen; OSMUNDO BRILHANTE OLIVEIRA‑NETO, Faculdade Planalto Central; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
Improved cotton transformation protocol mediated by Agrobacterium and biolistic combined-methods. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Planta, v. 254, 20, 2021. |
ISSN: |
1432-2048; 0032-0935 |
DOI: |
https://doi-org.ez103.periodicos.capes.gov.br/10.1007/s00425-021-03666-5 |
Idioma: |
Inglês |
Conteúdo: |
Cotton (Gossypium spp.) is the most important crop for natural textile fiber production worldwide. Nonetheless, one of the main challenges in cotton production are the losses resulting from insect pests, pathogens, and abiotic stresses. One effective way to solve these issues is to use genetically modified (GM) varieties. Herein, we describe an improved protocol for straightforward and cost-effective genetic transformation of cotton embryo axes, merging biolistics and Agrobacterium. The experimental steps include (1) Agrobacterium preparation, (2) seed sterilization, (3) cotton embryo excision, (4) lesion of shoot-cells by tungsten bombardment, (5) Agrobacterium-mediated transformation, (6) embryo co-culture, (7) regeneration and selection of transgenic plants in vitro, and (8) molecular characterization of plants. Due to the high regenerative power of the embryonic axis and the exceptional ability of the meristem cells for plant regeneration through organogenesis in vitro, this protocol can be performed in approximately 4?10 weeks, with an average plant regeneration of about 5.5% (±?0.53) and final average transformation efficiency of 60% (±?0.55). The transgene was stably inherited, and most transgenic plants hold a single copy of the transgene, as desirable and expected in Agrobacterium-mediated transformation. Additionally, the transgene was stably expressed over generations, and transgenic proteins could be detected at high levels in the T2 generation of GM cotton plants. The T2 progeny showed no phenotypic or productivity disparity compared to wild-type plants. Collectively, the use of cotton embryo axes and the enhanced DNA-delivery system by combining particle bombardment and Agrobacterium infection enabled efficient transgenic plant recovery, overcoming usual limitations associated with the recalcitrance of several cotton genotypes subjected to somatic embryogenesis. The improved approach states this method?s success for cotton genetic modification, allowing us to obtain GM cotton plants carrying traits, which are of fundamental relevance for the advancement of global agribusiness. MenosCotton (Gossypium spp.) is the most important crop for natural textile fiber production worldwide. Nonetheless, one of the main challenges in cotton production are the losses resulting from insect pests, pathogens, and abiotic stresses. One effective way to solve these issues is to use genetically modified (GM) varieties. Herein, we describe an improved protocol for straightforward and cost-effective genetic transformation of cotton embryo axes, merging biolistics and Agrobacterium. The experimental steps include (1) Agrobacterium preparation, (2) seed sterilization, (3) cotton embryo excision, (4) lesion of shoot-cells by tungsten bombardment, (5) Agrobacterium-mediated transformation, (6) embryo co-culture, (7) regeneration and selection of transgenic plants in vitro, and (8) molecular characterization of plants. Due to the high regenerative power of the embryonic axis and the exceptional ability of the meristem cells for plant regeneration through organogenesis in vitro, this protocol can be performed in approximately 4?10 weeks, with an average plant regeneration of about 5.5% (±?0.53) and final average transformation efficiency of 60% (±?0.55). The transgene was stably inherited, and most transgenic plants hold a single copy of the transgene, as desirable and expected in Agrobacterium-mediated transformation. Additionally, the transgene was stably expressed over generations, and transgenic proteins could be detected at high levels in the T2 generation of GM cotton plant... Mostrar Tudo |
Palavras-Chave: |
Embryonic axis; Genotype-independent transformation; Modificação genética do algodão. |
Thesagro: |
Agrobacterium Tumefaciens; Algodão; DNA; Gossypium Hirsutum; Método de Melhoramento; Planta Transgênica. |
Thesaurus Nal: |
Abiotic stress; Biolistics; Cotton; Genetic transformation; Genetically modified plants. |
Categoria do assunto: |
-- G Melhoramento Genético |
Marc: |
LEADER 03519naa a2200457 a 4500 001 2134441 005 2021-09-15 008 2021 bl uuuu u00u1 u #d 022 $a1432-2048; 0032-0935 024 7 $ahttps://doi-org.ez103.periodicos.capes.gov.br/10.1007/s00425-021-03666-5$2DOI 100 1 $aRIBEIRO, T. P. 245 $aImproved cotton transformation protocol mediated by Agrobacterium and biolistic combined-methods.$h[electronic resource] 260 $c2021 520 $aCotton (Gossypium spp.) is the most important crop for natural textile fiber production worldwide. Nonetheless, one of the main challenges in cotton production are the losses resulting from insect pests, pathogens, and abiotic stresses. One effective way to solve these issues is to use genetically modified (GM) varieties. Herein, we describe an improved protocol for straightforward and cost-effective genetic transformation of cotton embryo axes, merging biolistics and Agrobacterium. The experimental steps include (1) Agrobacterium preparation, (2) seed sterilization, (3) cotton embryo excision, (4) lesion of shoot-cells by tungsten bombardment, (5) Agrobacterium-mediated transformation, (6) embryo co-culture, (7) regeneration and selection of transgenic plants in vitro, and (8) molecular characterization of plants. Due to the high regenerative power of the embryonic axis and the exceptional ability of the meristem cells for plant regeneration through organogenesis in vitro, this protocol can be performed in approximately 4?10 weeks, with an average plant regeneration of about 5.5% (±?0.53) and final average transformation efficiency of 60% (±?0.55). The transgene was stably inherited, and most transgenic plants hold a single copy of the transgene, as desirable and expected in Agrobacterium-mediated transformation. Additionally, the transgene was stably expressed over generations, and transgenic proteins could be detected at high levels in the T2 generation of GM cotton plants. The T2 progeny showed no phenotypic or productivity disparity compared to wild-type plants. Collectively, the use of cotton embryo axes and the enhanced DNA-delivery system by combining particle bombardment and Agrobacterium infection enabled efficient transgenic plant recovery, overcoming usual limitations associated with the recalcitrance of several cotton genotypes subjected to somatic embryogenesis. The improved approach states this method?s success for cotton genetic modification, allowing us to obtain GM cotton plants carrying traits, which are of fundamental relevance for the advancement of global agribusiness. 650 $aAbiotic stress 650 $aBiolistics 650 $aCotton 650 $aGenetic transformation 650 $aGenetically modified plants 650 $aAgrobacterium Tumefaciens 650 $aAlgodão 650 $aDNA 650 $aGossypium Hirsutum 650 $aMétodo de Melhoramento 650 $aPlanta Transgênica 653 $aEmbryonic axis 653 $aGenotype-independent transformation 653 $aModificação genética do algodão 700 1 $aLOURENCO, I. T. 700 1 $aMELO, B. P. de 700 1 $aMORGANTE, C. V. 700 1 $aSALLES FILHO, A. 700 1 $aLINS, C. B. J. 700 1 $aFERREIRA, G. F. 700 1 $aMELLO, G. N. 700 1 $aMACEDO, L. L. P. de 700 1 $aLUCENA, W. A. 700 1 $aSILVA, M. C. M. da 700 1 $aOLIVEIRA‑NETO, O. B. 700 1 $aSA, M. F. G. de 773 $tPlanta$gv. 254, 20, 2021.
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Embrapa Semiárido (CPATSA) |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
08/02/2019 |
Data da última atualização: |
07/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
SOUSA, R. T. de; BOMFIM, M. A. D.; ALBUQUERQUE, F. H. M. A. R. de; SANTOS, C. M. dos; FERRARI, V. B.; FERNANDES, F. E. P.; FONSECA, J. F. da. |
Afiliação: |
RAFAEL TEIXEIRA DE SOUSA; MARCO AURELIO DELMONDES BOMFIM, CNPC; FERNANDO HENRIQUE M A R DE ALBUQUERQUE, CNPC; CLAUDIANE MORAIS DOS SANTOS, Universidade de São Paulo (USP) - Pirassununga, SP, Brasil; VIVIANE BORBA FERRARI; FRANCISCO EDEN PAIVA FERNANDES, CNPC; JEFERSON FERREIRA DA FONSECA, CNPC. |
Título: |
Corn-based or high linoleic acid flushing increases productivity of Morada Nova and Brazilian Somalis ewes. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Revista Brasileira de Saúde e Produção Animal, v. 20, 1-16, e0212019, 2019. |
DOI: |
http://dx.doi.org/10.1590/s1519-994020021201 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The objective of this study was to compare the corn-based (FLU), high linoleic acid flushings(LAF), and a control treatment (un supplemented, CON); two genetic groups:Morada Nova (MN) and Brazilian Somalis (BS), on the productive and reproductive performance of ewes and their lambs. A total of 156 non-pregnant ewes were supplemented three weeks before and after the breeding season. Supplementation with either FLU or LAF increased ewe body condition score (BCS) at the beginning of the breeding season and at lambing compared to CON. The BS ewes had greater body weights at the supplementation end, and BCS both at the supplementation start, and at lambing compared to MN. Similarly, ewes with three lambs per birth had greater BCS at the beginning of supplementation. They also had greater weights at the beginning of the breeding season and at lambing. Both FLU and LAF increased total lamb weight from 56 to 105 days of age compared to CON.The BS ewes had decreased prolificity and produced lower total weight of lambs at lambing, but greater 84 and 105 days after birth compared to MN. Total lambweight at birth was greater for ewes that lambed three, followed by two and then one lamb per birth. In conclusion, the high linoleic acid and high corn supplementation increase body condition score of ewes form breeding season to lambing and enhance lamb performance after 56 days of age. Although Morada Nova ewes increased prolificacy, their lambs were lighter after they reached 84 days of age. [Flushing à base de milho ou alto ácido linoleico aumenta a produtividade de ovelhas Morada Nova e Somalis Brasileira]. Resumo: O objetivo deste estudo foi comparar flushings à base de milho (FLU), ácido linoleico alto (FAL), e tratamento controle (não suplementado, CON);dois grupos genéticos: Morada Nova (MN) e Somalis Brasileiro (SB), sobre desempenho de ovelhas e cordeiros. Foram suplementadas 156 ovelhas não prenhes três semanas antes e após a estação de monta. Tanto FLU quanto FAL aumentaram o escore de condição corporal (ECC) de ovelhas no início da estação de monta e no parto, comparado ao CON. Ovelhas da raça SB tiveram maior peso no final da suplementação, e ECC, tanto no início da suplementação, quanto ao parto comparado ao MN. Da mesma forma, ovelhas com três cordeiros por nascimento tiveram ECC maior no início da suplementação. Elas também tiveram maiores pesos tanto no início da estação como no parto. Tanto FLU como FAL aumentaram o peso do cordeiro de 56 até 105 dias de idade comparadoao CON. Ovelhas SB diminuíram a prolificidade e produziram menor peso total de cordeiros ao parto, porém maior peso totalapós 84 e 105 dias comparado às ovelhas MN. O peso totaldecordeiros ao nascimento foi maior para ovelhas com gestação tripla, seguido por dupla, e simples. Em conclusão, a suplementação com ácido linoleico e milho aumentam o escore da condição corporal de ovelhas desde a estação de monta até o parto e a performance do cordeiro após 56 dias de idade. Embora ovelhas Morada Nova tenham aumentado a prolificidade, seus cordeiros ficaram mais leves apósos 84 dias de idade. MenosAbstract: The objective of this study was to compare the corn-based (FLU), high linoleic acid flushings(LAF), and a control treatment (un supplemented, CON); two genetic groups:Morada Nova (MN) and Brazilian Somalis (BS), on the productive and reproductive performance of ewes and their lambs. A total of 156 non-pregnant ewes were supplemented three weeks before and after the breeding season. Supplementation with either FLU or LAF increased ewe body condition score (BCS) at the beginning of the breeding season and at lambing compared to CON. The BS ewes had greater body weights at the supplementation end, and BCS both at the supplementation start, and at lambing compared to MN. Similarly, ewes with three lambs per birth had greater BCS at the beginning of supplementation. They also had greater weights at the beginning of the breeding season and at lambing. Both FLU and LAF increased total lamb weight from 56 to 105 days of age compared to CON.The BS ewes had decreased prolificity and produced lower total weight of lambs at lambing, but greater 84 and 105 days after birth compared to MN. Total lambweight at birth was greater for ewes that lambed three, followed by two and then one lamb per birth. In conclusion, the high linoleic acid and high corn supplementation increase body condition score of ewes form breeding season to lambing and enhance lamb performance after 56 days of age. Although Morada Nova ewes increased prolificacy, their lambs were lighter after they reached 84 ... Mostrar Tudo |
Palavras-Chave: |
Desempenho reprodutivo; Prolificidade; Suplementação; Supplementation. |
Thesagro: |
Ácido Graxo; Alimentação Suplementar; Alimento Para Animal; Eficiência Reprodutiva; Fertilidade; Nutrição Animal; Ovino; Performance; Produção Animal. |
Thesaurus NAL: |
Animal fertility; Animal nutrition; Animal production; Brazil; Fatty acids; Feed supplements; Reproductive performance; Sheep feeding. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192371/1/CNPC-2019-Corn-based.pdf
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Marc: |
LEADER 04563naa a2200457 a 4500 001 2105733 005 2019-11-07 008 2019 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1590/s1519-994020021201$2DOI 100 1 $aSOUSA, R. T. de 245 $aCorn-based or high linoleic acid flushing increases productivity of Morada Nova and Brazilian Somalis ewes.$h[electronic resource] 260 $c2019 520 $aAbstract: The objective of this study was to compare the corn-based (FLU), high linoleic acid flushings(LAF), and a control treatment (un supplemented, CON); two genetic groups:Morada Nova (MN) and Brazilian Somalis (BS), on the productive and reproductive performance of ewes and their lambs. A total of 156 non-pregnant ewes were supplemented three weeks before and after the breeding season. Supplementation with either FLU or LAF increased ewe body condition score (BCS) at the beginning of the breeding season and at lambing compared to CON. The BS ewes had greater body weights at the supplementation end, and BCS both at the supplementation start, and at lambing compared to MN. Similarly, ewes with three lambs per birth had greater BCS at the beginning of supplementation. They also had greater weights at the beginning of the breeding season and at lambing. Both FLU and LAF increased total lamb weight from 56 to 105 days of age compared to CON.The BS ewes had decreased prolificity and produced lower total weight of lambs at lambing, but greater 84 and 105 days after birth compared to MN. Total lambweight at birth was greater for ewes that lambed three, followed by two and then one lamb per birth. In conclusion, the high linoleic acid and high corn supplementation increase body condition score of ewes form breeding season to lambing and enhance lamb performance after 56 days of age. Although Morada Nova ewes increased prolificacy, their lambs were lighter after they reached 84 days of age. [Flushing à base de milho ou alto ácido linoleico aumenta a produtividade de ovelhas Morada Nova e Somalis Brasileira]. Resumo: O objetivo deste estudo foi comparar flushings à base de milho (FLU), ácido linoleico alto (FAL), e tratamento controle (não suplementado, CON);dois grupos genéticos: Morada Nova (MN) e Somalis Brasileiro (SB), sobre desempenho de ovelhas e cordeiros. Foram suplementadas 156 ovelhas não prenhes três semanas antes e após a estação de monta. Tanto FLU quanto FAL aumentaram o escore de condição corporal (ECC) de ovelhas no início da estação de monta e no parto, comparado ao CON. Ovelhas da raça SB tiveram maior peso no final da suplementação, e ECC, tanto no início da suplementação, quanto ao parto comparado ao MN. Da mesma forma, ovelhas com três cordeiros por nascimento tiveram ECC maior no início da suplementação. Elas também tiveram maiores pesos tanto no início da estação como no parto. Tanto FLU como FAL aumentaram o peso do cordeiro de 56 até 105 dias de idade comparadoao CON. Ovelhas SB diminuíram a prolificidade e produziram menor peso total de cordeiros ao parto, porém maior peso totalapós 84 e 105 dias comparado às ovelhas MN. O peso totaldecordeiros ao nascimento foi maior para ovelhas com gestação tripla, seguido por dupla, e simples. Em conclusão, a suplementação com ácido linoleico e milho aumentam o escore da condição corporal de ovelhas desde a estação de monta até o parto e a performance do cordeiro após 56 dias de idade. Embora ovelhas Morada Nova tenham aumentado a prolificidade, seus cordeiros ficaram mais leves apósos 84 dias de idade. 650 $aAnimal fertility 650 $aAnimal nutrition 650 $aAnimal production 650 $aBrazil 650 $aFatty acids 650 $aFeed supplements 650 $aReproductive performance 650 $aSheep feeding 650 $aÁcido Graxo 650 $aAlimentação Suplementar 650 $aAlimento Para Animal 650 $aEficiência Reprodutiva 650 $aFertilidade 650 $aNutrição Animal 650 $aOvino 650 $aPerformance 650 $aProdução Animal 653 $aDesempenho reprodutivo 653 $aProlificidade 653 $aSuplementação 653 $aSupplementation 700 1 $aBOMFIM, M. A. D. 700 1 $aALBUQUERQUE, F. H. M. A. R. de 700 1 $aSANTOS, C. M. dos 700 1 $aFERRARI, V. B. 700 1 $aFERNANDES, F. E. P. 700 1 $aFONSECA, J. F. da 773 $tRevista Brasileira de Saúde e Produção Animal$gv. 20, 1-16, e0212019, 2019.
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