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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
26/11/2004 |
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Data da última atualização: |
08/03/2010 |
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Autoria: |
FRANCO, R. W. A.; MARTIN-NETO, L.; KATO, M. S. A.; FURLAN, J. M. M. W.; COLNAGO, L. A. |
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Título: |
Identification of irradiation treatment in black pepper by electron paramagnetic resonance. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
International Journal of Food Science and Technology, Oxford, v.39, p.395-401, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
Electron paramagnetic resonance spectroscopy was applied to study free radicals in black pepper, and to evaluate the potential ofthese radicalsin identifying radiation treatment. A doublet signal was detected at g = 2.0042 and a = 1.83 mT, produced exclusively by radiolysis. This signal is as stab1e as that of irradiated cellulose, and can be used to identify recent radiation treatment. A line produced both by radiolysis and thermolysis was observed, and its behaviour with thermal treatments suggests that it cannot be used as an irradiation indicator for doses up to 30 kGy, contradicting a model proposed in lhe literature. |
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Palavras-Chave: |
EPR. |
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Thesagro: |
Alimento; Irradiação; Sabor. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01259naa a2200217 a 4500
001 1028687
005 2010-03-08
008 2004 bl --- 0-- u #d
100 1 $aFRANCO, R. W. A.
245 $aIdentification of irradiation treatment in black pepper by electron paramagnetic resonance.
260 $c2004
520 $aElectron paramagnetic resonance spectroscopy was applied to study free radicals in black pepper, and to evaluate the potential ofthese radicalsin identifying radiation treatment. A doublet signal was detected at g = 2.0042 and a = 1.83 mT, produced exclusively by radiolysis. This signal is as stab1e as that of irradiated cellulose, and can be used to identify recent radiation treatment. A line produced both by radiolysis and thermolysis was observed, and its behaviour with thermal treatments suggests that it cannot be used as an irradiation indicator for doses up to 30 kGy, contradicting a model proposed in lhe literature.
650 $aAlimento
650 $aIrradiação
650 $aSabor
653 $aEPR
700 1 $aMARTIN-NETO, L.
700 1 $aKATO, M. S. A.
700 1 $aFURLAN, J. M. M. W.
700 1 $aCOLNAGO, L. A.
773 $tInternational Journal of Food Science and Technology, Oxford$gv.39, p.395-401, 2004.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
20/08/2021 |
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Data da última atualização: |
20/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SAMPAIO, L.; ANTONIO, R. P.; NASCIMENTO, M. N. do; FERNANDES JUNIOR, P. I. |
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Afiliação: |
LUCAS SAMPAIO; RAFAELA PRISCILA ANTONIO, CPATSA; MARILZA NEVES DO NASCIMENTO; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Molecular approaches for identification of the apomictic/sexual reproductive mechanism and genetic variability in buffel grass (Cenchrus spp.) accessions. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Genetika, v. 53, n. 2, p. 493-505, 2021. |
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DOI: |
https://doi.org/10.2298/GENSR2102493S |
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Idioma: |
Inglês |
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Conteúdo: |
Cenchrus spp. encompasses forage grasses that are especially important for drylands. Thus, information on their reproductive mechanism and genetic diversity is needed. The Active Germplasm Bank (AGB) of the Embrapa Semiárido (Tropical semiarid research center) has 115 accessions of Cenchrus spp. that were not molecularly characterized. Therefore, the objective of this work was to evaluate the genetic diversity of Cenchrus spp. accessions in the AGB, and identify their reproductive mechanisms using DNA markers. Specific SCAR markers Q8H, UGT197, and PCAB10, in addition to the SCAR marker 4HS* were used to identify genotypes with apomictic and sexual reproduction modes, respectively; the genetic diversity was characterized using the ISSR D12 (GA) 6CG and HB14 (CTC)3GC markers. The reproductive mechanism was analyzed using images for the presence or absence of the band in the expected amplicon size. For the analysis of genetic diversity, the gel images were exported to the BioNumerics Software (Applied Maths), in which polymorphism data were obtained and the dissimilarity dendrogram constructed by the UPGMA clustering method using the Dice coefficient. The markers related to the apomictic region were positive for all accessions. However, only the accessions CPATSA 102 and CPATSA 134 had positive markers using the primers for both reproduction strategies, indicating that these individuals can be facultative sexual. The cluster analysis discriminated two large groups and the accessions clustered mainly by the place of origin. It was also possible to identify duplicates within AGB. The identification and exclusion of duplicate accessions provides financial and labor savings in maintaining and managing the AGB. The two sexual accessions were selected for the breeding programs of Cenchrus spp. in Brazil. MenosCenchrus spp. encompasses forage grasses that are especially important for drylands. Thus, information on their reproductive mechanism and genetic diversity is needed. The Active Germplasm Bank (AGB) of the Embrapa Semiárido (Tropical semiarid research center) has 115 accessions of Cenchrus spp. that were not molecularly characterized. Therefore, the objective of this work was to evaluate the genetic diversity of Cenchrus spp. accessions in the AGB, and identify their reproductive mechanisms using DNA markers. Specific SCAR markers Q8H, UGT197, and PCAB10, in addition to the SCAR marker 4HS* were used to identify genotypes with apomictic and sexual reproduction modes, respectively; the genetic diversity was characterized using the ISSR D12 (GA) 6CG and HB14 (CTC)3GC markers. The reproductive mechanism was analyzed using images for the presence or absence of the band in the expected amplicon size. For the analysis of genetic diversity, the gel images were exported to the BioNumerics Software (Applied Maths), in which polymorphism data were obtained and the dissimilarity dendrogram constructed by the UPGMA clustering method using the Dice coefficient. The markers related to the apomictic region were positive for all accessions. However, only the accessions CPATSA 102 and CPATSA 134 had positive markers using the primers for both reproduction strategies, indicating that these individuals can be facultative sexual. The cluster analysis discriminated two large groups and the acce... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética; Seleção assistida por marcadores. |
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Thesagro: |
Apomixia; Capim Buffel; Germoplasma; Gramínea Forrageira; Melhoramento Genético Animal; Pastagem. |
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Thesaurus NAL: |
Apomixis; Breeding; Cenchrus; Forage grasses; Germplasm; Pastures. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02870naa a2200337 a 4500
001 2133769
005 2021-08-20
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.2298/GENSR2102493S$2DOI
100 1 $aSAMPAIO, L.
245 $aMolecular approaches for identification of the apomictic/sexual reproductive mechanism and genetic variability in buffel grass (Cenchrus spp.) accessions.$h[electronic resource]
260 $c2021
520 $aCenchrus spp. encompasses forage grasses that are especially important for drylands. Thus, information on their reproductive mechanism and genetic diversity is needed. The Active Germplasm Bank (AGB) of the Embrapa Semiárido (Tropical semiarid research center) has 115 accessions of Cenchrus spp. that were not molecularly characterized. Therefore, the objective of this work was to evaluate the genetic diversity of Cenchrus spp. accessions in the AGB, and identify their reproductive mechanisms using DNA markers. Specific SCAR markers Q8H, UGT197, and PCAB10, in addition to the SCAR marker 4HS* were used to identify genotypes with apomictic and sexual reproduction modes, respectively; the genetic diversity was characterized using the ISSR D12 (GA) 6CG and HB14 (CTC)3GC markers. The reproductive mechanism was analyzed using images for the presence or absence of the band in the expected amplicon size. For the analysis of genetic diversity, the gel images were exported to the BioNumerics Software (Applied Maths), in which polymorphism data were obtained and the dissimilarity dendrogram constructed by the UPGMA clustering method using the Dice coefficient. The markers related to the apomictic region were positive for all accessions. However, only the accessions CPATSA 102 and CPATSA 134 had positive markers using the primers for both reproduction strategies, indicating that these individuals can be facultative sexual. The cluster analysis discriminated two large groups and the accessions clustered mainly by the place of origin. It was also possible to identify duplicates within AGB. The identification and exclusion of duplicate accessions provides financial and labor savings in maintaining and managing the AGB. The two sexual accessions were selected for the breeding programs of Cenchrus spp. in Brazil.
650 $aApomixis
650 $aBreeding
650 $aCenchrus
650 $aForage grasses
650 $aGermplasm
650 $aPastures
650 $aApomixia
650 $aCapim Buffel
650 $aGermoplasma
650 $aGramínea Forrageira
650 $aMelhoramento Genético Animal
650 $aPastagem
653 $aDiversidade genética
653 $aSeleção assistida por marcadores
700 1 $aANTONIO, R. P.
700 1 $aNASCIMENTO, M. N. do
700 1 $aFERNANDES JUNIOR, P. I.
773 $tGenetika$gv. 53, n. 2, p. 493-505, 2021.
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Embrapa Semiárido (CPATSA) |
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