Data/hora: 04/06/2024 15:07:36

Biblioteca(s): | Embrapa Semiárido.
Data corrente: | 20/08/2021
Data da última atualização: | 20/08/2021
Autoria: | SAMPAIO, L.; ANTONIO, R. P.; NASCIMENTO, M. N. do; FERNANDES JUNIOR, P. I.
Afiliação: | LUCAS SAMPAIO; RAFAELA PRISCILA ANTONIO, CPATSA; MARILZA NEVES DO NASCIMENTO; PAULO IVAN FERNANDES JUNIOR, CPATSA.
Título: | Molecular approaches for identification of the apomictic/sexual reproductive mechanism and genetic variability in buffel grass (Cenchrus spp.) accessions.
Ano de publicação: | 2021
Fonte/Imprenta: | Genetika, v. 53, n. 2, p. 493-505, 2021.
DOI: | https://doi.org/10.2298/GENSR2102493S
Idioma: | Inglês
Conteúdo: | Cenchrus spp. encompasses forage grasses that are especially important for drylands. Thus, information on their reproductive mechanism and genetic diversity is needed. The Active Germplasm Bank (AGB) of the Embrapa Semiárido (Tropical semiarid research center) has 115 accessions of Cenchrus spp. that were not molecularly characterized. Therefore, the objective of this work was to evaluate the genetic diversity of Cenchrus spp. accessions in the AGB, and identify their reproductive mechanisms using DNA markers. Specific SCAR markers Q8H, UGT197, and PCAB10, in addition to the SCAR marker 4HS* were used to identify genotypes with apomictic and sexual reproduction modes, respectively; the genetic diversity was characterized using the ISSR D12 (GA) 6CG and HB14 (CTC)3GC markers. The reproductive mechanism was analyzed using images for the presence or absence of the band in the expected amplicon size. For the analysis of genetic diversity, the gel images were exported to the BioNumerics Software (Applied Maths), in which polymorphism data were obtained and the dissimilarity dendrogram constructed by the UPGMA clustering method using the Dice coefficient. The markers related to the apomictic region were positive for all accessions. However, only the accessions CPATSA 102 and CPATSA 134 had positive markers using the primers for both reproduction strategies, indicating that these individuals can be facultative sexual. The cluster analysis discriminated two large groups and the accessions clustered mainly by the place of origin. It was also possible to identify duplicates within AGB. The identification and exclusion of duplicate accessions provides financial and labor savings in maintaining and managing the AGB. The two sexual accessions were selected for the breeding programs of Cenchrus spp. in Brazil.
Palavras-Chave: | Diversidade genética; Seleção assistida por marcadores.
Thesagro: | Apomixia; Capim Buffel; Germoplasma; Gramínea Forrageira; Melhoramento Genético Animal; Pastagem.
Thesaurus Nal: | Apomixis; Breeding; Cenchrus; Forage grasses; Germplasm; Pastures.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Semiárido (CPATSA)
Exemplares: | CPATSA - (59615-1) - UPC - AP - DD -  -  -  - DIS