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Registro Completo |
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
25/09/1998 |
Data da última atualização: |
25/09/1998 |
Autoria: |
LAM, K. M.; YAMAMOTO, R.; DaMASSA, A. J. |
Título: |
DNA diversity among isolates of campylobacter jejuni detected by PCR-based RAPD fingerprinting |
Ano de publicação: |
1995 |
Fonte/Imprenta: |
Veterinary Microbiology, v.45, n.2/3, p.269-274, 1995. |
Idioma: |
Inglês |
Conteúdo: |
A PCR-based randomly amplified polymorphic DNA method was used to amplify Campylobacter jejuni DNA using a single oligonucleotide primer derived from either a homologous source or from mycoplasma gallisepticum.The method was able to detect the heterogeneity of amplified DNA from human, chicken and turkey sources and can be used as a tool to study the epidemiology of Campytobacter jejuni infection. |
Palavras-Chave: |
Amostragem randomica; Diversity; Fingerprinting; RAPD. |
Thesagro: |
Bactéria; DNA. |
Thesaurus Nal: |
Campylobacter jejuni. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01027naa a2200229 a 4500 001 1370009 005 1998-09-25 008 1995 bl --- 0-- u #d 100 1 $aLAM, K. M. 245 $aDNA diversity among isolates of campylobacter jejuni detected by PCR-based RAPD fingerprinting 260 $c1995 520 $aA PCR-based randomly amplified polymorphic DNA method was used to amplify Campylobacter jejuni DNA using a single oligonucleotide primer derived from either a homologous source or from mycoplasma gallisepticum.The method was able to detect the heterogeneity of amplified DNA from human, chicken and turkey sources and can be used as a tool to study the epidemiology of Campytobacter jejuni infection. 650 $aCampylobacter jejuni 650 $aBactéria 650 $aDNA 653 $aAmostragem randomica 653 $aDiversity 653 $aFingerprinting 653 $aRAPD 700 1 $aYAMAMOTO, R. 700 1 $aDaMASSA, A. J. 773 $tVeterinary Microbiology$gv.45, n.2/3, p.269-274, 1995.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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Registro Completo
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
15/02/2017 |
Data da última atualização: |
29/12/2017 |
Autoria: |
GUERRA FILHO, J. B. P.; YAMATOGI, R. S.; POSSEBON, F. S.; FERNANDES, S. A.; TIBA-CASAS, M. R.; LARA, G. H. B.; RIBEIRO, M. G.; PINTO, J. P. A. N. |
Afiliação: |
JOÃO B. P. GUERRA FILHO, UNESP/FMVZ; RICARDO S. YAMATOGI, UNESP/FMVZ; FÁBIO S. POSSEBON, UNESP/FMVZ; SUELI A. FERNANDES, Instituto Adolfo Lutz; MONIQUE R. TIBA-CASAS, Instituto Adolfo Lutz; GUSTAVO H. B. LARA, UNESP/FMVZ; MÁRCIO G. RIBEIRO, UNESP/FMVZ; JOSÉ P. A. N. PINTO, UNESP/FMVZ. |
Título: |
Frequency, serotyping and antimicrobial resistance pattern of Salmonella from feces and lymph nodes of pigs. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 12, p. 1165-1170, dez. 2016. |
Idioma: |
Português |
Conteúdo: |
Salmonellosis is a foodborne disease caused by bacteria of the genus Salmonella, being pigs and pork-products potentially important for its occurrence. In recent decades, some serovars of Salmonella have shown increase of resistance to conventional antimicrobials used in human and animal therapy, with serious risks for public health. The aim of this study was to evaluate feces (n=50), mediastinal (n=50), mesenteric (n=50) and mandibular (n=50) lymph nodes obtained from slaughter houses for Salmonella spp. Positive samples were serotyped and subjected to an in vitro antimicrobial susceptibility test, including the extended-spectrum beta-lactamase (ESBL) production. Salmonella species were identified in 10% (20/200) of total samples. From these, 20% (10/50) were identified in the submandibular lymph nodes, 18% (9/50) in the mesenteric lymph nodes, 2% (1/50) in feces and 0% (0/50) in the mediastinal lymph nodes. The serotypes found were Salonella Typhimurium (55%), S. enterica subsp. enterica 4,5,12: i: - (35%), S. Brandenburg and S. Derby with 5% (5% each). All strains showed resistance to at least one antimicrobial; 90% were resistant to four or more antimicrobials, and 15% were multidrug-resistant. Resistance to ciprofloxacin, tetracycline and nalidixic acid was particularly prevalent amongst the tested serovars. Here, we highlighted the impact of pigs in the epidemiological chain of salmonellosis in domestic animals and humans, as well as the high antimicrobial resistance rates of Salmonella strains, reinforcing the necessity for responsible use of antimicrobials for animals as an emergent One Health issue, and to keep these drugs for human therapy approaches. MenosSalmonellosis is a foodborne disease caused by bacteria of the genus Salmonella, being pigs and pork-products potentially important for its occurrence. In recent decades, some serovars of Salmonella have shown increase of resistance to conventional antimicrobials used in human and animal therapy, with serious risks for public health. The aim of this study was to evaluate feces (n=50), mediastinal (n=50), mesenteric (n=50) and mandibular (n=50) lymph nodes obtained from slaughter houses for Salmonella spp. Positive samples were serotyped and subjected to an in vitro antimicrobial susceptibility test, including the extended-spectrum beta-lactamase (ESBL) production. Salmonella species were identified in 10% (20/200) of total samples. From these, 20% (10/50) were identified in the submandibular lymph nodes, 18% (9/50) in the mesenteric lymph nodes, 2% (1/50) in feces and 0% (0/50) in the mediastinal lymph nodes. The serotypes found were Salonella Typhimurium (55%), S. enterica subsp. enterica 4,5,12: i: - (35%), S. Brandenburg and S. Derby with 5% (5% each). All strains showed resistance to at least one antimicrobial; 90% were resistant to four or more antimicrobials, and 15% were multidrug-resistant. Resistance to ciprofloxacin, tetracycline and nalidixic acid was particularly prevalent amongst the tested serovars. Here, we highlighted the impact of pigs in the epidemiological chain of salmonellosis in domestic animals and humans, as well as the high antimicrobial resistance r... Mostrar Tudo |
Palavras-Chave: |
Antimicrobiano; Linfonodo; Multidroga resistente; Multidrug-resistant; Serotyping antimicrobial resistance; Sorotipagem; Sorovar. |
Thesagro: |
Epidemiologia; Fezes; Isolamento; Resistência; Salmonella; Suíno. |
Thesaurus NAL: |
Epidemiology; Feces; Lymph nodes; Pork; Serotypes; Swine. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155751/1/Frequency-serotyping-and-antimicrobial.pdf
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Marc: |
LEADER 02910naa a2200433 a 4500 001 2064289 005 2017-12-29 008 2016 bl uuuu u00u1 u #d 100 1 $aGUERRA FILHO, J. B. P. 245 $aFrequency, serotyping and antimicrobial resistance pattern of Salmonella from feces and lymph nodes of pigs.$h[electronic resource] 260 $c2016 520 $aSalmonellosis is a foodborne disease caused by bacteria of the genus Salmonella, being pigs and pork-products potentially important for its occurrence. In recent decades, some serovars of Salmonella have shown increase of resistance to conventional antimicrobials used in human and animal therapy, with serious risks for public health. The aim of this study was to evaluate feces (n=50), mediastinal (n=50), mesenteric (n=50) and mandibular (n=50) lymph nodes obtained from slaughter houses for Salmonella spp. Positive samples were serotyped and subjected to an in vitro antimicrobial susceptibility test, including the extended-spectrum beta-lactamase (ESBL) production. Salmonella species were identified in 10% (20/200) of total samples. From these, 20% (10/50) were identified in the submandibular lymph nodes, 18% (9/50) in the mesenteric lymph nodes, 2% (1/50) in feces and 0% (0/50) in the mediastinal lymph nodes. The serotypes found were Salonella Typhimurium (55%), S. enterica subsp. enterica 4,5,12: i: - (35%), S. Brandenburg and S. Derby with 5% (5% each). All strains showed resistance to at least one antimicrobial; 90% were resistant to four or more antimicrobials, and 15% were multidrug-resistant. Resistance to ciprofloxacin, tetracycline and nalidixic acid was particularly prevalent amongst the tested serovars. Here, we highlighted the impact of pigs in the epidemiological chain of salmonellosis in domestic animals and humans, as well as the high antimicrobial resistance rates of Salmonella strains, reinforcing the necessity for responsible use of antimicrobials for animals as an emergent One Health issue, and to keep these drugs for human therapy approaches. 650 $aEpidemiology 650 $aFeces 650 $aLymph nodes 650 $aPork 650 $aSerotypes 650 $aSwine 650 $aEpidemiologia 650 $aFezes 650 $aIsolamento 650 $aResistência 650 $aSalmonella 650 $aSuíno 653 $aAntimicrobiano 653 $aLinfonodo 653 $aMultidroga resistente 653 $aMultidrug-resistant 653 $aSerotyping antimicrobial resistance 653 $aSorotipagem 653 $aSorovar 700 1 $aYAMATOGI, R. S. 700 1 $aPOSSEBON, F. S. 700 1 $aFERNANDES, S. A. 700 1 $aTIBA-CASAS, M. R. 700 1 $aLARA, G. H. B. 700 1 $aRIBEIRO, M. G. 700 1 $aPINTO, J. P. A. N. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 12, p. 1165-1170, dez. 2016.
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