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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
04/03/2016 |
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Data da última atualização: |
04/03/2016 |
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Autoria: |
LEMOS, V. F.; GUARANÁ, E. L. S.; AFONSO, J. A. B.; FAGLIARI, J. J.; SILVA, P. C.; SOARES P. C.; MENDONÇA, C. L. de. |
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Afiliação: |
VÂNIA F. LEMOS, UFRPE; EDUARDO L. S. GUARANÁ, UFRPE; JOSÉ A. B. AFONSO, UFRPE; JOSÉ J. FAGLIARI, UNESP; PAULO C. SILVA, UNESP; PIERRE C. SOARES, UFRPE; CARLA LOPES DE MENDONÇA, UFRPE. |
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Título: |
Effects of intramammary infection on whey proteinograms os sheep during lactation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 3, p. 230-236, mar. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
The study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for IgG LC and ?-lactoalbumin. The most significant changes were observed in ?-1- acid glycoprotein, lactoferrin and haptoglobin, which showed three-, five-, and seven-fold increases in secretion, respectively. This study showed that haptoglobin, ?-1-acid glycoprotein, lactoferrin, albumin, and the IgA and IgG immunoglobulins may serve as potential biomarkers for mammary gland infection in sheep. MenosThe study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for ... Mostrar Tudo |
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Palavras-Chave: |
Marcador biológico; Mastite subclínica; Proteína de fase aguda; Subclinical mastitis. |
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Thesagro: |
Leite; Ovelha. |
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Thesaurus Nal: |
Acute phase proteins; Biomarkers; Ewes; Milk. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140638/1/Effects-of-intramammary-infection.pdf
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Marc: |
LEADER 02780naa a2200313 a 4500
001 2039647
005 2016-03-04
008 2015 bl uuuu u00u1 u #d
100 1 $aLEMOS, V. F.
245 $aEffects of intramammary infection on whey proteinograms os sheep during lactation.
260 $c2015
520 $aThe study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for IgG LC and ?-lactoalbumin. The most significant changes were observed in ?-1- acid glycoprotein, lactoferrin and haptoglobin, which showed three-, five-, and seven-fold increases in secretion, respectively. This study showed that haptoglobin, ?-1-acid glycoprotein, lactoferrin, albumin, and the IgA and IgG immunoglobulins may serve as potential biomarkers for mammary gland infection in sheep.
650 $aAcute phase proteins
650 $aBiomarkers
650 $aEwes
650 $aMilk
650 $aLeite
650 $aOvelha
653 $aMarcador biológico
653 $aMastite subclínica
653 $aProteína de fase aguda
653 $aSubclinical mastitis
700 1 $aGUARANÁ, E. L. S.
700 1 $aAFONSO, J. A. B.
700 1 $aFAGLIARI, J. J.
700 1 $aSILVA, P. C.
700 1 $aSOARES P. C.
700 1 $aMENDONÇA, C. L. de
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 3, p. 230-236, mar. 2015.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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| Registros recuperados : 9 | |
| 1. |  | FERNANDES, C. D.; BATISTA, M. V.; FERNANDES, R. de C.; SALLES, N. E. P. C.; SORGATTO, M. Efeito da quimioterapia na nematofauna fitopatogênica de sementes de Brachiaria brizantha cv. Marandu. Fitopatologia Brasileira, Brasília, DF, v. 32, p. S185, ago. 2007. Suplemento, ref 0378. Edição dos resumos do XL Congresso Brasileiro de Fitopatologia, 2007, Maringá. CNPGC.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 2. | | SALLES, N. E. P. C.; MARCHI, C. E.; FERNANDES, C. D.; BATISTA, M. V.; SORGATTO, M.; FERNANDES, R. DE C.; BARBOSA, C. S. Efeito da quimioterapia na microflora fúngica de sementes de Brachiaria brizantha cv. Marandu. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 3., 2007, Campo Grande, MS. Anais [da]... Campo Grande, MS: Embrapa Gado de Corte, 2007. p. 65 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 3. | | SALLES, N. E. P. C.; MARCHI, C. E.; FERNANDES, C. D.; BATISTA, M. V.; SORGATTO, M.; FERNANDES, R. de C.; BARBOSA, C. S. Efeito da quimioterapia na microflora fúngica de sementes de Brachiaria brizantha cv. Marandu. Fitopatologia Brasileira, Brasília, DF, v. 32, p. S221, ago. 2007. Suplemento, res. 0557. Edição dos resumos do XL Congresso Brasileiro de Fitopatologia, 2007, Maringá.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | BARBOSA, C. S.; FERNANDES, C. D.; SALLES, N. E. P. C.; MARCHI, C. E.; BATISTA, M. V.; SORGATTO, M.; FERNANDES, R. DE C.; PEREIRA, K. R. F. Incidência de fungos associados a sementes comerciais de Panicum maximum cvs. Massai, Mombaça e Tanzânia. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 3., 2007, Campo Grande, MS. Anais [da]... Campo Grande, MS: Embrapa Gado de Corte, 2007. p. 80 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 5. | | SORGATTO, M.; MARCHI, C. E.; FERNANDES, C. D.; FERNANDES, R. de C.; BATISTA, M. V.; SALLES, N. E. P. C.; BARBOSA, C. S. Ocorrência de escleródios de Claviceps maximensis em sementes de Brachiaria brizantha cv. Xaraés. Fitopatologia Brasileira, Brasília, DF, v. 32, p. S173, ago. 2007. Suplemento, ref 0316. Edição dos resumos do XL Congresso Brasileiro de Fitopatologia, 2007, Maringá. CNPGC.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 6. | | SORGATTO, M.; MARCHI; C. E.; FERNANDES, C. D.; BATISTA, M. V.; SALLES, N. E. P. C.; FERNANDES, R. DE C.; BARBOSA, C. S. Ocorrência e morfologia de escleródios de Claviceps maximensis em sementes de Brachiaria brizantha cv. Xaraés. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 3., 2007, Campo Grande, MS. Anais [da]... Campo Grande, MS: Embrapa Gado de Corte, 2007. p. 73 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 7. | | SALLES, N. E. P. C.; FERNANDES, C. D.; MARCHI, C. E.; COSTA, M. L. N.; BATISTA, M. V.; SORGATTO, M.; BARBOSA, C. da S.; PEREIRA, K. R. F.; FERNANDES, R. de C. Efeito da quimioterapia sobre a microflora fúngica de sementes de Brachiaria brizantha cv. Xaraés. In: SEMINÁRIO INTERNO DE INICIAÇÃO CIENTÍFICA DA UNIDERP, 2., 2007, Campo Grande, MS. Anais... Campo Grande, MS: Ed. da UNIDERP, 2007. 3 p. 1 CD-ROM. CNPGC.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 8. | | PEREIRA, K. R. F.; FERNANDES, C. D.; MARCHI, C. E.; COSTA, M. L. N.; BATISTA, M. V.; SORGATTO, M.; BARBOSA, C. da S.; SALLES, N. E. P. C.; FERNANDES, R. de C. Identificação de fitonematóides em sementes de forrageiras comerciais e avaliação da termoterapia para o seu controle. In: SEMINÁRIO INTERNO DE INICIAÇÃO CIENTÍFICA DA UNIDERP, 2., 2007, Campo Grande, MS. Anais... Campo Grande, MS: Ed. da UNIDERP, 2007. 4 p. 1 CD-ROM. CNPGC.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 9. | | SORGATTO, M.; MARCHI, C. E.; FERNANDES, C. D.; VECHIATO, M. H.; COSTA, M. L. N.; SALLES, N. E. P. C.; BATISTA, M. V.; FERNANDES, R. de C.; BARBOSA, C. da S. Estudo morfológico e fisiológico dos escleródios de Claviceps maximensis de Brachiaria brizantha cv. Xaraés. Summa Phytopathologica, Botucatu, v. 34, S32, 2008. Suplemento. Edição dos Resumos do 31. Congresso Paulista de Fitopatologia, Campinas, fev. 2008. Resumo 082.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 9 | |
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