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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
31/05/2005 |
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Data da última atualização: |
28/07/2005 |
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Autoria: |
DIAS, S. C.; FRANCO, O. L.; MAGALHÃES, C. P.; OLIVEIRA-NETO, O. B. de; ALUMANN, R. A.; FIGUEIRA, E. L. Z.; MELO, F. R.; GROSSI-de-SÁ, M. F. |
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Título: |
Molecular cloning and expression of an alpha- amylase inhibitor from rye with potential for controlling insect pests. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
The Protein Journal, v. 24, n. 2, p. 113-123, Feb. 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
Alpha-amylase inhibitors have important roles in plant defense mechanisms, particularly against insects, and several of these inhibitors have been expressed in different crops to increase their resistance to particular insects. In this work, we report the cloning and expression of a gene encoding for a new -amylase inhibitor (BIII) from rye (Secale cereale) seeds. The BIII gene contains 354 nucleotides that encode for 118 amino acids sequence. A 313 bp fragment of the gene was expressed in Escherichia coli and resulted in a functional inhibitor that reduced the activity of -amylases of larvae of the coleopteran pests Acanthoscelides obtectus, Zabrotess subfasciatus and Anthonomus grandis. In contrast, the inhibitor did not inhibit the activity of porcine pancreatic -amylase. Although the amino acid sequence of BIII showed high identity with those of bifunctional inhibitors, the recombinant protein was unable to inhibit trypsin-like serine proteinases. The effects of recombinant BIII were evaluated in vivo against A. grandis. When first instar larvae were reared on an artificial diet containing four different concentrations of BIII, a reduction in larval weight and a mortality of 83% were observed at the highest concentration.
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Palavras-Chave: |
Alfa amilase; Clonagem molecular; Proteína CM. |
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Thesagro: |
Defesa Vegetal; Inibidor de Enzima. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02034naa a2200265 a 4500
001 1185701
005 2005-07-28
008 2005 bl --- 0-- u #d
100 1 $aDIAS, S. C.
245 $aMolecular cloning and expression of an alpha- amylase inhibitor from rye with potential for controlling insect pests.
260 $c2005
520 $aAlpha-amylase inhibitors have important roles in plant defense mechanisms, particularly against insects, and several of these inhibitors have been expressed in different crops to increase their resistance to particular insects. In this work, we report the cloning and expression of a gene encoding for a new -amylase inhibitor (BIII) from rye (Secale cereale) seeds. The BIII gene contains 354 nucleotides that encode for 118 amino acids sequence. A 313 bp fragment of the gene was expressed in Escherichia coli and resulted in a functional inhibitor that reduced the activity of -amylases of larvae of the coleopteran pests Acanthoscelides obtectus, Zabrotess subfasciatus and Anthonomus grandis. In contrast, the inhibitor did not inhibit the activity of porcine pancreatic -amylase. Although the amino acid sequence of BIII showed high identity with those of bifunctional inhibitors, the recombinant protein was unable to inhibit trypsin-like serine proteinases. The effects of recombinant BIII were evaluated in vivo against A. grandis. When first instar larvae were reared on an artificial diet containing four different concentrations of BIII, a reduction in larval weight and a mortality of 83% were observed at the highest concentration.
650 $aDefesa Vegetal
650 $aInibidor de Enzima
653 $aAlfa amilase
653 $aClonagem molecular
653 $aProteína CM
700 1 $aFRANCO, O. L.
700 1 $aMAGALHÃES, C. P.
700 1 $aOLIVEIRA-NETO, O. B. de
700 1 $aALUMANN, R. A.
700 1 $aFIGUEIRA, E. L. Z.
700 1 $aMELO, F. R.
700 1 $aGROSSI-de-SÁ, M. F.
773 $tThe Protein Journal$gv. 24, n. 2, p. 113-123, Feb. 2005.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
12/06/2015 |
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Data da última atualização: |
25/05/2017 |
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Autoria: |
FERNANDES, F. L.; FERNANDES, M. E. de S. |
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Afiliação: |
FLÁVIO LEMES FERNANDES, UFV; MARIA ELISA DE SENA FERNANDES, UFV. |
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Título: |
Flight movement and spatial distribution of immunomarked thrips in onion, potato, and tomato. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 50, n. 5, p. 388-496, maio 2015. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Movimento de voo e distribuição espacial de tripes imunomarcados em cebola, batata e tomate. |
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Conteúdo: |
The objective of this work was to evaluate the spatial distribution of thrips in different crops, and the correlation between meterological parameters and the flight movements of this pest, using immunomarking. The experiment was conducted in cultivated areas, with tomato (Solanum lycopersicum), potato (Solanum tuberosum), and onion (Allium cepa); and non?cultivated areas, with weedy plants. The areas with tomato (100 days), potato (20 days), and weeds were sprayed with casein, albumin, and soy milk, respectively, to mark adult thrips; however, the areas with onion (50 days) and tomato (10 days) were not sprayed. Thrips were captured with georeferenced blue sticky traps, transferred into tubes, and identified by treatment area with the Elisa test. The dependence between the samples and the capture distance was determined using geostatistics. Meteorlogical parameters were correlated with thrips density in each area. The three protein types used for immunomarking were detected in different proportions in the thrips. There was a correlation between casein?marked thrips and wind speed. The thrips flew a maximum distance of 3.5 km and dispersed from the older (tomato) to the younger crops (potato). The immunomarking method is efficient to mark large quantities of thrips. |
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Palavras-Chave: |
Conrole integrado. |
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Thesagro: |
Elisa. |
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Thesaurus NAL: |
Amaryllidaceae; Enzyme-linked immunosorbent assay; Integrated pest management; Solanaceae; Thysanoptera. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/125302/1/Flight-movement-and-spatial.pdf
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Marc: |
LEADER 02100naa a2200229 a 4500
001 2017561
005 2017-05-25
008 2015 bl uuuu u00u1 u #d
100 1 $aFERNANDES, F. L.
245 $aFlight movement and spatial distribution of immunomarked thrips in onion, potato, and tomato.
260 $c2015
500 $aTítulo em português: Movimento de voo e distribuição espacial de tripes imunomarcados em cebola, batata e tomate.
520 $aThe objective of this work was to evaluate the spatial distribution of thrips in different crops, and the correlation between meterological parameters and the flight movements of this pest, using immunomarking. The experiment was conducted in cultivated areas, with tomato (Solanum lycopersicum), potato (Solanum tuberosum), and onion (Allium cepa); and non?cultivated areas, with weedy plants. The areas with tomato (100 days), potato (20 days), and weeds were sprayed with casein, albumin, and soy milk, respectively, to mark adult thrips; however, the areas with onion (50 days) and tomato (10 days) were not sprayed. Thrips were captured with georeferenced blue sticky traps, transferred into tubes, and identified by treatment area with the Elisa test. The dependence between the samples and the capture distance was determined using geostatistics. Meteorlogical parameters were correlated with thrips density in each area. The three protein types used for immunomarking were detected in different proportions in the thrips. There was a correlation between casein?marked thrips and wind speed. The thrips flew a maximum distance of 3.5 km and dispersed from the older (tomato) to the younger crops (potato). The immunomarking method is efficient to mark large quantities of thrips.
650 $aAmaryllidaceae
650 $aEnzyme-linked immunosorbent assay
650 $aIntegrated pest management
650 $aSolanaceae
650 $aThysanoptera
650 $aElisa
653 $aConrole integrado
700 1 $aFERNANDES, M. E. de S.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 50, n. 5, p. 388-496, maio 2015.
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Embrapa Unidades Centrais (AI-SEDE) |
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