Data/hora: 21/06/2024 05:29:26

Biblioteca(s): 	Embrapa Gado de Corte; Embrapa Unidades Centrais.	
Data corrente: 	30/01/2017	
Data da última atualização: 	07/03/2017	
Autoria: 	SANCHES, C. C.; ROSINHA, G. M. S.; GALVÃO, C. E.; FEIJO, G. L. D.; ARAUJO, F. R.; SOARES, C. O.	
Afiliação: 	CRISTIANE C. SANCHES, UFMS; GRACIA MARIA SOARES ROSINHA, CNPGC; CLEER E. GALVÃO, UFMS; GELSON LUIS DIAS FEIJO, CNPGC; FLABIO RIBEIRO ARAUJO, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC.	
Título: 	Polymorphisms in the prion protein gene of cattle breeds from Brazil.	
Ano de publicação: 	2016	
Fonte/Imprenta: 	Pesquisa Veterinária Brasileira, Brasília, DF, v. 36, n. 11, p. 1059-1066, nov. 2016.	
Idioma: 	Inglês	
Conteúdo: 	One of the alterations that occur in the PRNP gene in bovines is the insertion/deletion (indel) of base sequences in specific regions, such as indels of 12-base pairs (bp) in intron 1 and of 23- bp in the promoter region. The deletion allele of 23 bp is associated with susceptibility to bovine spongiform encephalopathy (BSE) as well as the presence of the deletion allele of 12 bp. In the present study, the variability of nucleotides in the promoter region and intron 1 of the PRNP gene was genotyped for the Angus, Canchim, Nellore and Simmental bovine breeds to identify the genotype profiles of resistance and/or susceptibility to BSE in each animal. Genomic DNA was extracted for amplification of the target regions of the PRNP gene using polymerase chain reaction (PCR) and specific primers. The PCR products were submitted to electrophoresis in agarose gel 3% and sequencing for genotyping. With the exception of the Angus breed, most breeds exhibited a higher frequency of deletion alleles for 12 bp and 23 bp in comparison to their respective insertion alleles for both regions. These results represent an important contribution to understanding the formation process of the Brazilian herd in relation to bovine PRNP gene polymorphisms.	
Palavras-Chave: 	Indel; PRNP.	
Thesagro: 	Bovino; Doença da vaca louca; Polimorfismo.	
Thesaurus Nal: 	Bovine spongiform encephalopathy; Brazil; Cattle; Prions.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/154345/1/Polymorphisms-in-the-prion.pdf
http://ainfo.cnptia.embrapa.br/digital/bitstream/item/154252/1/Polymorphisms-in-the-prion.pdf	
Registro original: 	Embrapa Gado de Corte (CNPGC)	
Exemplares: 	AI-SEDE - (60634-1) - UPE - AP - DD -  -  -  - DIS
CNPGC - (16719-1) - UPC - AP - DD -  -  -  - DIS