Data/hora: 09/06/2024 11:29:31

Biblioteca(s): | Embrapa Gado de Leite; Embrapa Pecuária Sudeste.
Data corrente: | 06/04/2020
Data da última atualização: | 20/04/2020
Autoria: | OLIVEIRA, F. A. de; VIGNA, B. B. Z.; SILVA, C. C. da; FAVERO, A. P.; MATTA, F. de P.; AZEVEDO, A. L. S.; SOUZA, A. P. de.
Afiliação: | Fernanda A. de Oliveira, UNICAMP; BIANCA BACCILI ZANOTTO VIGNA, CPPSE; Carla C. da Silva, UNICAMP; ALESSANDRA PEREIRA FAVERO, CPPSE; FREDERICO DE PINA MATTA, CPPSE; ANA LUISA SOUSA AZEVEDO, CNPGL; Anete P. de Souza, UNICAMP.
Título: | Coexpression and transcriptome analyses identify active apomixis-related genes in Paspalum notatum leaves.
Ano de publicação: | 2020
Fonte/Imprenta: | BMC Genomics, v. 21, n. 78, 2020.
Páginas: | 15 p.
DOI: | https://doi.org/10.1186/s12864-020-6518-z
Idioma: | Inglês
Conteúdo: | Background - Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum cytotypes to identify differential expression patterns and candidate genes involved in the regulation of expression of this trait. Results - We built a comprehensive transcriptome using leaf and inflorescence from apomictic tetraploids and sexual diploids/tetraploids and a coexpression network based on pairwise correlations between transcript expression profiles. We identified genes exclusively expressed in each cytotype and genes differentially expressed between pairs of cytotypes. Gene Ontology enrichment analyses were performed to better interpret the data. We de novo assembled 114,306 reference transcripts. In total, 536 candidate genes possibly associated with apomixis were detected through statistical analyses of the differential expression data, and several interacting genes potentially linked to the apomixis-controlling region, genes that have already been reported in the literature, and their neighbors were transcriptionally related in the coexpression network. Conclusions - Apomixis is a highly desirable trait in modern agriculture due to the maintenance of the characteristics of the mother plant in the progeny. The reference transcriptome, candidate genes and their coexpression network identified in this work represent rich resources for future grass breeding programs.
Palavras-Chave: | Differential expression; Gene coexpression network; RNA sequencing.
Thesaurus Nal: | Apomixis; Paspalum.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia

URL:

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/212171/1/CoexpressionTranscriptomeAnalyses.pdf
http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1129868/1/Coexpression-and-transcriptome-analyses-identify-active-apomixis-related-genes-in-Paspalum.pdf

Registro original: | Embrapa Pecuária Sudeste (CPPSE)

Exemplares:

CPPSE - (25097-1) - UPC - AP - DD - PROCI-2020.00024 - OLI - 2020.00069 - DIS
CNPGL - (25091-1) - UPC - AP - DD -  -  -  - DIS