|
|
Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
27/05/2016 |
Data da última atualização: |
29/01/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
TULLIO, L. D.; PAULITSCH, F.; REICHERT, P. R. S.; KLEPA, M. S.; KLEPA, M. S.; PILEGGI, M.; GOMES, D. F.; HUNGRIA, M.; BATISTA, J. S. S. |
Afiliação: |
UEPG; UEPG; UEPG; UEPG; UEPG; MARIANGELA HUNGRIA DA CUNHA, CNPSO; UEPG. |
Título: |
Proteomic analysis of Rhizobium freirei PRF 81 responses to low pH. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28.; SIMPÓSIO DE FERMENTAÇÃO ALCOÓLICA, 3.; SIMPÓSIO DE MICRORGANISMOS FOTOSSINTETIZANTES, 3.; SIMPÓSIO DE ESCHERICHIA COLI LUIZ RACHID TRABULSI, 4., 2015, Florianópolis. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2015. |
Idioma: |
Português |
Notas: |
Res. 1407-2. |
Conteúdo: |
Rhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution digitized gel images and analyzed by Image Master 2D Platinum v 5.0 software. Relative volumes (%vol) of compared between the two conditions tested and were statistically evaluated (p ≤ 0.05). Even knowing that R. freirei PRF 81 can still grow in more acid conditions, pH 4.8 was chosen because didn´t affect significantly the bacterial growth kinetics, a factor that could compromise the analysis. Using a narrow pH range, the gel profiles displayed a better resolution and reprodutibility than using broader pH range. Spots were mostly concentrated between pH 5-7 and molecular masses between 17-95 kDa. From the six hundred well-defined spots analyzed, one hundred and sixty-three spots presented a significant change in % vol, indicating that the pH led to expressive changes in the proteome of R. freirei PRF 81. Of these, sixty-one were up-regulated and one hundred two was downregulated in pH 4.8 condition. Also, fourteen spots were only identified in the acid condition, while seven spots was exclusively detected in pH 6.8. Ninety-five differentially expressed spots and two exclusively detected in pH 4,8 were selected for Maldi-Tof identification. Together with the genome sequencing and the proteome analysis of heat stress, we will search for molecular determinants of PRF 81 related to capacity to adapt to stressful tropical conditions. MenosRhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution digitized gel images and analyzed by Image Master 2D Platinum v 5.0 software. Relative volumes (%vol) of compared between the two conditions tested and were statistically evaluated (p ≤ 0.05). Even knowing that R. freirei PRF 81 can still grow in more acid conditions, pH 4.8 was chosen because didn´t affect significantly the bacterial growth kinetics, a factor that could compromise the analysis. Using a narrow pH range, the gel profiles displayed a better resolution and reprodutibility than using broader pH range. Spots were mostly concentrated between pH 5-7 and molecular masses between 17-95 kDa. From the six hundred well-defined spots analyzed, one hundred and sixty-three spots presented a significant chan... Mostrar Tudo |
Thesagro: |
Fixação de nitrogênio. |
Thesaurus Nal: |
Nitrogen fixation. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143444/1/R1407-2.PDF
|
Marc: |
LEADER 03060nam a2200241 a 4500 001 2045758 005 2018-01-29 008 2015 bl uuuu u00u1 u #d 100 1 $aTULLIO, L. D. 245 $aProteomic analysis of Rhizobium freirei PRF 81 responses to low pH.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28.; SIMPÓSIO DE FERMENTAÇÃO ALCOÓLICA, 3.; SIMPÓSIO DE MICRORGANISMOS FOTOSSINTETIZANTES, 3.; SIMPÓSIO DE ESCHERICHIA COLI LUIZ RACHID TRABULSI, 4., 2015, Florianópolis. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia$c2015 500 $aRes. 1407-2. 520 $aRhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution digitized gel images and analyzed by Image Master 2D Platinum v 5.0 software. Relative volumes (%vol) of compared between the two conditions tested and were statistically evaluated (p ≤ 0.05). Even knowing that R. freirei PRF 81 can still grow in more acid conditions, pH 4.8 was chosen because didn´t affect significantly the bacterial growth kinetics, a factor that could compromise the analysis. Using a narrow pH range, the gel profiles displayed a better resolution and reprodutibility than using broader pH range. Spots were mostly concentrated between pH 5-7 and molecular masses between 17-95 kDa. From the six hundred well-defined spots analyzed, one hundred and sixty-three spots presented a significant change in % vol, indicating that the pH led to expressive changes in the proteome of R. freirei PRF 81. Of these, sixty-one were up-regulated and one hundred two was downregulated in pH 4.8 condition. Also, fourteen spots were only identified in the acid condition, while seven spots was exclusively detected in pH 6.8. Ninety-five differentially expressed spots and two exclusively detected in pH 4,8 were selected for Maldi-Tof identification. Together with the genome sequencing and the proteome analysis of heat stress, we will search for molecular determinants of PRF 81 related to capacity to adapt to stressful tropical conditions. 650 $aNitrogen fixation 650 $aFixação de nitrogênio 700 1 $aPAULITSCH, F. 700 1 $aREICHERT, P. R. S. 700 1 $aKLEPA, M. S. 700 1 $aKLEPA, M. S. 700 1 $aPILEGGI, M. 700 1 $aGOMES, D. F. 700 1 $aHUNGRIA, M. 700 1 $aBATISTA, J. S. S.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Soja (CNPSO) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
01/08/2017 |
Data da última atualização: |
14/03/2019 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
JUNQUEIRA, N. T. V.; FAVARO, S. P.; BRAGA, M. F.; CONCEIÇÃO, L. D. H. C. S.; ANTONIASSI, R.; CICONINI, G.; SILVA, D. B. da. |
Afiliação: |
NILTON TADEU VILELA JUNQUEIRA, CPAC; SIMONE PALMA FAVARO, CNPAE; MARCELO FIDELES BRAGA, CPAC; LEO DUC HAA CARSON SCHWARTZHAUPT DA, CPAC; ROSEMAR ANTONIASSI, CTAA; GABRIELLY CICONINI, UNIVERSIDADE CATÓLICA DOM BOSCO; DIJALMA BARBOSA DA SILVA, CENARGEN. |
Título: |
Acrocomia spp.: macaúba. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: VIEIRA, R. F.; CAMILLO, J.; CORADIN, L. (Ed.). Espécies nativas da flora brasileira de valor econômico atual ou potencial: plantas para o futuro: Região Centro-Oeste. Brasília, DF: MMA, 2018. 1.160 p. (Série Biodiversidade; 44). |
Páginas: |
p. 119-137 |
Idioma: |
Português |
Palavras-Chave: |
Acrocomia glaucescens Lorenzi; Acrocomia hassleri; Acrocomia totai Mart. |
Thesagro: |
Acrocomia Aculeata; Acrocomia Sclerocarpa; Bocaiúva; Cerrado; Espécie nativa; Macaúba. |
Thesaurus NAL: |
Arecaceae; Arecales. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01046naa a2200325 a 4500 001 2073417 005 2019-03-14 008 2018 bl uuuu u00u1 u #d 100 1 $aJUNQUEIRA, N. T. V. 245 $aAcrocomia spp.$bmacaúba. 260 $c2018 300 $ap. 119-137 650 $aArecaceae 650 $aArecales 650 $aAcrocomia Aculeata 650 $aAcrocomia Sclerocarpa 650 $aBocaiúva 650 $aCerrado 650 $aEspécie nativa 650 $aMacaúba 653 $aAcrocomia glaucescens Lorenzi 653 $aAcrocomia hassleri 653 $aAcrocomia totai Mart 700 1 $aFAVARO, S. P. 700 1 $aBRAGA, M. F. 700 1 $aCONCEIÇÃO, L. D. H. C. S. 700 1 $aANTONIASSI, R. 700 1 $aCICONINI, G. 700 1 $aSILVA, D. B. da 773 $tIn: VIEIRA, R. F.; CAMILLO, J.; CORADIN, L. (Ed.). Espécies nativas da flora brasileira de valor econômico atual ou potencial: plantas para o futuro: Região Centro-Oeste. Brasília, DF: MMA, 2018. 1.160 p. (Série Biodiversidade; 44).
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|