|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
05/07/2023 |
Data da última atualização: |
15/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TEODORO, K. B. R.; ALVARENGA, A. D.; OLIVEIRA, L. F. R.; CHAGAS, P. A. M.; LOPES. R. G.; ANDRE, R. S.; MERCANTE, L. A.; ALVES, F.; STRINGASCI, M. D.; BUZZA, H. H.; INADA, N. M.; CORREA, D. S. |
Afiliação: |
DANIEL SOUZA CORREA, CNPDIA. |
Título: |
Fast Fabrication of Multifunctional PCL/Curcumin Nanofibrous Membranes for Wound Dressings. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
ACS Applied Bio Materials, v. 6, 2023. |
Páginas: |
2325–2337 |
DOI: |
https://doi.org/10.1021/acsabm.3c00177 |
Idioma: |
Inglês |
Conteúdo: |
herapeutic intervention to skin wounds requires covering the affected area with wound dressings. Interdisciplinary efforts have focused on the development of smart bandages that can perform multiple functions. In this direction, here, we designed a low cost (U$0.012 per cm2 ) multifunctional therapeutic wound dressing fabricated by loading curcumin (CC) into poly(ϵ-caprolactone) (PCL) nanofibers using solution blow spinning (SBS). The freestanding PCL/CC bandages were characterized by distinct physicochemical approaches and were successful in performing varied functions, including controlled release of CC, colorimetric indication of the wound conditions, barrier against microorganisms, being biocompatible, and providing a photosensitive platform for antimicrobial photodynamic therapy (aPDT). The chemical nature of PCL and CC and the interactions between these components allowed CC to be released for 192 h (ca. 8 days), which could be correlated with the Korsmeyer−Peppas model, with a burst release suitable to treat the inflammatory phase. Due to the CC keto−enol tautomerism, an optical indication of the healing status could be obtained using PCL/CC, which occurred immediately, ranging between red/orange and yellow shades. The effect against pathogenic microorganisms evaluated by agar disc-diffusion, affected skin wound simulation (ex vivo), and microbial penetration tests demonstrated the ability to block and inhibit microbial permeation in different environments. The biocompatibilities of PCL and PCL/CC were verified by in vitro cytotoxicity study, which demonstrated that cell viabilities average above 94 and 96% for human dermal fibroblasts. In addition, the proposed bandage responded to aPDT applied to an in vivo assay, showing that, when irritated, PCL/CC was able to reduce the bacteria present on the real wound of mice. In summary, our findings demonstrate that using PCL and CC to produce nonwovens by the SBS technique offers potential for the rapid fabrication of biocompatible and multifunctional wound dressings, paving the way for large-scale production and utilization of such dressings in the treatment of skin wounds. Menosherapeutic intervention to skin wounds requires covering the affected area with wound dressings. Interdisciplinary efforts have focused on the development of smart bandages that can perform multiple functions. In this direction, here, we designed a low cost (U$0.012 per cm2 ) multifunctional therapeutic wound dressing fabricated by loading curcumin (CC) into poly(ϵ-caprolactone) (PCL) nanofibers using solution blow spinning (SBS). The freestanding PCL/CC bandages were characterized by distinct physicochemical approaches and were successful in performing varied functions, including controlled release of CC, colorimetric indication of the wound conditions, barrier against microorganisms, being biocompatible, and providing a photosensitive platform for antimicrobial photodynamic therapy (aPDT). The chemical nature of PCL and CC and the interactions between these components allowed CC to be released for 192 h (ca. 8 days), which could be correlated with the Korsmeyer−Peppas model, with a burst release suitable to treat the inflammatory phase. Due to the CC keto−enol tautomerism, an optical indication of the healing status could be obtained using PCL/CC, which occurred immediately, ranging between red/orange and yellow shades. The effect against pathogenic microorganisms evaluated by agar disc-diffusion, affected skin wound simulation (ex vivo), and microbial penetration tests demonstrated the ability to block and inhibit microbial permeation in different enviro... Mostrar Tudo |
Palavras-Chave: |
Natural dye; Optical sensor; Photodynamic therapy; Solution blow spinning; Wound dressing. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03154naa a2200337 a 4500 001 2154815 005 2024-01-15 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1021/acsabm.3c00177$2DOI 100 1 $aTEODORO, K. B. R. 245 $aFast Fabrication of Multifunctional PCL/Curcumin Nanofibrous Membranes for Wound Dressings.$h[electronic resource] 260 $c2023 300 $a2325–2337 520 $aherapeutic intervention to skin wounds requires covering the affected area with wound dressings. Interdisciplinary efforts have focused on the development of smart bandages that can perform multiple functions. In this direction, here, we designed a low cost (U$0.012 per cm2 ) multifunctional therapeutic wound dressing fabricated by loading curcumin (CC) into poly(ϵ-caprolactone) (PCL) nanofibers using solution blow spinning (SBS). The freestanding PCL/CC bandages were characterized by distinct physicochemical approaches and were successful in performing varied functions, including controlled release of CC, colorimetric indication of the wound conditions, barrier against microorganisms, being biocompatible, and providing a photosensitive platform for antimicrobial photodynamic therapy (aPDT). The chemical nature of PCL and CC and the interactions between these components allowed CC to be released for 192 h (ca. 8 days), which could be correlated with the Korsmeyer−Peppas model, with a burst release suitable to treat the inflammatory phase. Due to the CC keto−enol tautomerism, an optical indication of the healing status could be obtained using PCL/CC, which occurred immediately, ranging between red/orange and yellow shades. The effect against pathogenic microorganisms evaluated by agar disc-diffusion, affected skin wound simulation (ex vivo), and microbial penetration tests demonstrated the ability to block and inhibit microbial permeation in different environments. The biocompatibilities of PCL and PCL/CC were verified by in vitro cytotoxicity study, which demonstrated that cell viabilities average above 94 and 96% for human dermal fibroblasts. In addition, the proposed bandage responded to aPDT applied to an in vivo assay, showing that, when irritated, PCL/CC was able to reduce the bacteria present on the real wound of mice. In summary, our findings demonstrate that using PCL and CC to produce nonwovens by the SBS technique offers potential for the rapid fabrication of biocompatible and multifunctional wound dressings, paving the way for large-scale production and utilization of such dressings in the treatment of skin wounds. 653 $aNatural dye 653 $aOptical sensor 653 $aPhotodynamic therapy 653 $aSolution blow spinning 653 $aWound dressing 700 1 $aALVARENGA, A. D. 700 1 $aOLIVEIRA, L. F. R. 700 1 $aCHAGAS, P. A. M. 700 1 $aLOPES. R. G. 700 1 $aANDRE, R. S. 700 1 $aMERCANTE, L. A. 700 1 $aALVES, F. 700 1 $aSTRINGASCI, M. D. 700 1 $aBUZZA, H. H. 700 1 $aINADA, N. M. 700 1 $aCORREA, D. S. 773 $tACS Applied Bio Materials$gv. 6, 2023.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
25/09/2019 |
Data da última atualização: |
12/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
AMORIM, C. C.; FARINAS, C. S.; MIRANDA, E. A. |
Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
Título: |
Liquefied wheat bran as carbon source and inducer in high-solids submerged cultivation of Aspergillus niger for xylanase production. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Biocatalysis and Agricultural Biotechnology, V. 21, 2019, 101346. |
Páginas: |
6 p. |
ISSN: |
1878-8181 |
DOI: |
10.1016/j.bcab.2019.101346 |
Idioma: |
Inglês |
Palavras-Chave: |
Submerged cultivation; Xylanase. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00647naa a2200193 a 4500 001 2112483 005 2019-11-12 008 2019 bl uuuu u00u1 u #d 022 $a1878-8181 024 7 $a10.1016/j.bcab.2019.101346$2DOI 100 1 $aAMORIM, C. C. 245 $aLiquefied wheat bran as carbon source and inducer in high-solids submerged cultivation of Aspergillus niger for xylanase production.$h[electronic resource] 260 $c2019 300 $a6 p. 653 $aSubmerged cultivation 653 $aXylanase 700 1 $aFARINAS, C. S. 700 1 $aMIRANDA, E. A. 773 $tBiocatalysis and Agricultural Biotechnology, V. 21, 2019, 101346.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|