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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
03/10/2022 |
Data da última atualização: |
13/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MOREIRA, V. J. V; LOURENCO, I. T.; BASSO, M. F.; SÁ. M. E. L. de; MORGANTE, C. V.; MELO, B. P.; ARRAES, F. B. M.; SÁ, D. M. de; SILVA, M. C. M. da; ENGLER, J. de A.; SA, M. F. G. de. |
Afiliação: |
VALDEIR JUNIO VAZ MOREIRA, UnB; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; MARCOS FERNANDO BASSO, National Institute of Science and Technology - INCT; MARIA EUGÊNIA LISEI DE SA, UnB; CAROLINA VIANNA MORGANTE, CPATSA; BRUNO PAES DE MELO, UFV; FABRÍCIO BARBOSA MONTEIRO ARRAES, National Institute of Science and Technology - INCT; DIOGO MARTINS DE SA, UnB; MARIA CRISTINA MATTAR DA SILVA, Cenargen; JANICE DE ALMEIDA ENGLER, National Institute of Science and Technology - INCT; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
Minc03328 effector gene down regulation severely affects Meloidogyne incognita parasitism in transgenic Arabidopsis thaliana. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Planta, v. 255, n. 2, 2022. |
Idioma: |
Inglês |
Conteúdo: |
Minc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Meloidogyne incognita is the most economically important species of root-knot nematodes (RKN) and causes severe damage to crops worldwide. M. incognita secretes several effector proteins to suppress the host plant defense response, and manipulate the plant cell cycle and other plant processes facilitating its parasitism. Different secreted effector proteins have already been identified in M. incognita, but not all have been characterized or have had the confirmation of their involvement in nematode parasitism in their host plants. Herein, we characterized the Minc03328 (Minc3s00020g01299) effector gene, confirmed its higher expression in the early stages of M. incognita parasitism in plants, as well as the accumulation of the Minc03328 effector protein in subventral glands and its secretion. We also discuss the potential for simultaneous downregulation of its paralogue Minc3s00083g03984 gene. Using the in planta RNA interference strategy, Arabidopsis thaliana plants overexpressing double-stranded RNA (dsRNA) were generated to specifically targeting and downregulating the Minc03328 gene during nematode parasitism. Transgenic Minc03328-dsRNA lines that significantly downregulated Minc03328 gene expression during M. incognita parasitism were significantly less susceptible. The number of galls, egg masses, and [galls/egg masses] ratio were reduced in these transgenic lines by up to 85%, 90%, and 87%, respectively. Transgenic Minc03328-dsRNA lines showed the presence of fewer and smaller galls, indicating that parasitism was hindered. Overall, data herein strongly suggest that Minc03328 effector protein is important for M. incognita parasitism establishment. As well, the in planta Minc03328-dsRNA strategy demonstrated high biotechnological potential for developing crop species that could efficiently control RKN in the field. MenosMinc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Meloidogyne incognita is the most economically important species of root-knot nematodes (RKN) and causes severe damage to crops worldwide. M. incognita secretes several effector proteins to suppress the host plant defense response, and manipulate the plant cell cycle and other plant processes facilitating its parasitism. Different secreted effector proteins have already been identified in M. incognita, but not all have been characterized or have had the confirmation of their involvement in nematode parasitism in their host plants. Herein, we characterized the Minc03328 (Minc3s00020g01299) effector gene, confirmed its higher expression in the early stages of M. incognita parasitism in plants, as well as the accumulation of the Minc03328 effector protein in subventral glands and its secretion. We also discuss the potential for simultaneous downregulation of its paralogue Minc3s00083g03984 gene. Using the in planta RNA interference strategy, Arabidopsis thaliana plants overexpressing double-stranded RNA (dsRNA) were generated to specifically targeting and downregulating the Minc03328 gene during nematode parasitism. Transgenic Minc03328-dsRNA lines that significantly downregulated Minc03328 ge... Mostrar Tudo |
Palavras-Chave: |
Interação planta-nematóide; Minc03328; Nematoide das galhas; Novas ferramentas biotecnológica; Planta RNAi; Proteção de cultivos; Proteína efetora. |
Thesagro: |
Meloidogyne Incognita; Nematóide; Planta. |
Thesaurus Nal: |
Plants (botany); Root-knot nematodes. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1147069/1/Minc03328-effector-gene-downregulation-2022.pdf
|
Marc: |
LEADER 03276naa a2200385 a 4500 001 2147069 005 2023-01-13 008 2022 bl uuuu u00u1 u #d 100 1 $aMOREIRA, V. J. V 245 $aMinc03328 effector gene down regulation severely affects Meloidogyne incognita parasitism in transgenic Arabidopsis thaliana.$h[electronic resource] 260 $c2022 520 $aMinc03328 effector gene downregulation triggered by in planta RNAi strategy strongly reduced plant susceptibility to Meloidogyne incognita and suggests that Minc03328 gene is a promising target for the development of genetically engineered crops to improve plant tolerance to M. incognita. Meloidogyne incognita is the most economically important species of root-knot nematodes (RKN) and causes severe damage to crops worldwide. M. incognita secretes several effector proteins to suppress the host plant defense response, and manipulate the plant cell cycle and other plant processes facilitating its parasitism. Different secreted effector proteins have already been identified in M. incognita, but not all have been characterized or have had the confirmation of their involvement in nematode parasitism in their host plants. Herein, we characterized the Minc03328 (Minc3s00020g01299) effector gene, confirmed its higher expression in the early stages of M. incognita parasitism in plants, as well as the accumulation of the Minc03328 effector protein in subventral glands and its secretion. We also discuss the potential for simultaneous downregulation of its paralogue Minc3s00083g03984 gene. Using the in planta RNA interference strategy, Arabidopsis thaliana plants overexpressing double-stranded RNA (dsRNA) were generated to specifically targeting and downregulating the Minc03328 gene during nematode parasitism. Transgenic Minc03328-dsRNA lines that significantly downregulated Minc03328 gene expression during M. incognita parasitism were significantly less susceptible. The number of galls, egg masses, and [galls/egg masses] ratio were reduced in these transgenic lines by up to 85%, 90%, and 87%, respectively. Transgenic Minc03328-dsRNA lines showed the presence of fewer and smaller galls, indicating that parasitism was hindered. Overall, data herein strongly suggest that Minc03328 effector protein is important for M. incognita parasitism establishment. As well, the in planta Minc03328-dsRNA strategy demonstrated high biotechnological potential for developing crop species that could efficiently control RKN in the field. 650 $aPlants (botany) 650 $aRoot-knot nematodes 650 $aMeloidogyne Incognita 650 $aNematóide 650 $aPlanta 653 $aInteração planta-nematóide 653 $aMinc03328 653 $aNematoide das galhas 653 $aNovas ferramentas biotecnológica 653 $aPlanta RNAi 653 $aProteção de cultivos 653 $aProteína efetora 700 1 $aLOURENCO, I. T. 700 1 $aBASSO, M. F. 700 1 $aSÁ. M. E. L. de 700 1 $aMORGANTE, C. V. 700 1 $aMELO, B. P. 700 1 $aARRAES, F. B. M. 700 1 $aSÁ, D. M. de 700 1 $aSILVA, M. C. M. da 700 1 $aENGLER, J. de A. 700 1 $aSA, M. F. G. de 773 $tPlanta$gv. 255, n. 2, 2022.
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Embrapa Semiárido (CPATSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Cerrados; Embrapa Soja. |
Data corrente: |
11/08/2006 |
Data da última atualização: |
19/03/2008 |
Autoria: |
FARIAS NETO, A. L. de; MOREIRA, C. T.; SOUZA, P. I. M. de; ABUD, S.; ALMEIDA, L. A. de; TOLEDO, J. F. F.; NUNES JUNIOR, J.; MONTEIRO, P. M. F. O.; DI STEFANO, J. G.; ASSUNÇÃO, M. S.; ARANTES, N. E.; YORINORI, J. T.; TEIXEIRA, R. N.; DIAS, W. P.; ALMEIDA, A. M. S.; TOLEDO, R. M. C. P. |
Título: |
Comportamento e descrição da linhagem transgênica BRASD 00-12057 RR no estado de Goiás e no Distrito Federal. |
Ano de publicação: |
2006 |
Fonte/Imprenta: |
In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 28., 2006, Uberaba. Resumos... Londrina: Embrapa Soja: Fundação Meridional: Fundação Triângulo, 2006. |
Páginas: |
p. 358-359. |
Série: |
(Embrapa Soja. Documentos, 272). |
Idioma: |
Português |
Notas: |
Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Boas de Campos Leite, Janete Lasso Ortiz. |
Palavras-Chave: |
Transgênico. |
Thesagro: |
Glycine Max; Linhagem; Soja; Variedade. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01311naa a2200385 a 4500 001 1469515 005 2008-03-19 008 2006 bl uuuu u00u1 u #d 100 1 $aFARIAS NETO, A. L. de 245 $aComportamento e descrição da linhagem transgênica BRASD 00-12057 RR no estado de Goiás e no Distrito Federal. 260 $c2006 300 $ap. 358-359. 490 $a(Embrapa Soja. Documentos, 272). 500 $aOrganizado por Odilon Ferreira Saraiva, Regina Maria Villas Boas de Campos Leite, Janete Lasso Ortiz. 650 $aGlycine Max 650 $aLinhagem 650 $aSoja 650 $aVariedade 653 $aTransgênico 700 1 $aMOREIRA, C. T. 700 1 $aSOUZA, P. I. M. de 700 1 $aABUD, S. 700 1 $aALMEIDA, L. A. de 700 1 $aTOLEDO, J. F. F. 700 1 $aNUNES JUNIOR, J. 700 1 $aMONTEIRO, P. M. F. O. 700 1 $aDI STEFANO, J. G. 700 1 $aASSUNÇÃO, M. S. 700 1 $aARANTES, N. E. 700 1 $aYORINORI, J. T. 700 1 $aTEIXEIRA, R. N. 700 1 $aDIAS, W. P. 700 1 $aALMEIDA, A. M. S. 700 1 $aTOLEDO, R. M. C. P. 773 $tIn: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 28., 2006, Uberaba. Resumos... Londrina: Embrapa Soja: Fundação Meridional: Fundação Triângulo, 2006.
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