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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
25/06/2018 |
Data da última atualização: |
10/01/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MEGIAS, E.; CARMO, L. S. T. do; NICOLINI, C.; SILVA, L. P. |
Afiliação: |
ESAU MEGIAS; LÍLIAN SILVEIRA TRAVASSOS DO CARMO; CÍCERO NICOLINI, UESPI; LUCIANO PAULINO DA SILVA, Cenargen. |
Título: |
Chloroplast Proteome of Nicotiana benthamiana Infected by Tomato Blistering Mosaic Virus. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
The Protein Journal, v. 37, p. 290-299, 2018. |
DOI: |
10.1007/s10930-018-9775-9 |
Idioma: |
Inglês |
Palavras-Chave: |
2-DE; Plant proteomics; Plant-virus interaction; ToBMV. |
Thesaurus Nal: |
Mass spectrometry. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179029/1/Megias2018-Article-ChloroplastProteomeOfNicotiana.pdf
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Marc: |
LEADER 00651naa a2200217 a 4500 001 2092764 005 2019-01-10 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1007/s10930-018-9775-9$2DOI 100 1 $aMEGIAS, E. 245 $aChloroplast Proteome of Nicotiana benthamiana Infected by Tomato Blistering Mosaic Virus.$h[electronic resource] 260 $c2018 650 $aMass spectrometry 653 $a2-DE 653 $aPlant proteomics 653 $aPlant-virus interaction 653 $aToBMV 700 1 $aCARMO, L. S. T. do 700 1 $aNICOLINI, C. 700 1 $aSILVA, L. P. 773 $tThe Protein Journal$gv. 37, p. 290-299, 2018.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
31/05/2016 |
Data da última atualização: |
29/12/2017 |
Autoria: |
FARIAS, T. H. V.; PEREIRA, N. L.; PÁDUA, S. B. de; ALVES, L. de O.; SAKABE, R.; BELO, M. A. de A.; PILARSKI, F. |
Afiliação: |
THAÍS HELOISA VAZ FARIAS, CAUnesp; NYCOLAS LEVY PEREIRA, CAUnesp; SANTIAGO BENITES DE PÁDUA, AquiVet Saúde Aquática; LINDOMAR DE OLIVEIRA ALVES, CAUnesp; ROBERSON SAKABE, UFF; MARCO ANTONIO DE ANDRADE BELO, UNESP; FABIANA PILARSKI, CAUnesp. |
Título: |
Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 431-435, maio. 2016. |
Idioma: |
Inglês |
Conteúdo: |
The present study aimed to evaluate the effects of Na heparin and Na2EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm). Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na2EDTA 10%, Na2EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na2EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na2EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na2EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na2EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood. |
Palavras-Chave: |
EDTA; Fish hematology; Hematologia de peixe; Pacus. |
Thesagro: |
Coagulação Sangüínea; Heparina. |
Thesaurus NAL: |
Blood coagulation; EDTA (chelating agent); Heparin; Piaractus mesopotamicus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143607/1/Na2EDTA-anticoagulant.pdf
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Marc: |
LEADER 02085naa a2200313 a 4500 001 2045983 005 2017-12-29 008 2016 bl uuuu u00u1 u #d 100 1 $aFARIAS, T. H. V. 245 $aNa2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus. 260 $c2016 520 $aThe present study aimed to evaluate the effects of Na heparin and Na2EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm). Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na2EDTA 10%, Na2EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na2EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na2EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na2EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na2EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood. 650 $aBlood coagulation 650 $aEDTA (chelating agent) 650 $aHeparin 650 $aPiaractus mesopotamicus 650 $aCoagulação Sangüínea 650 $aHeparina 653 $aEDTA 653 $aFish hematology 653 $aHematologia de peixe 653 $aPacus 700 1 $aPEREIRA, N. L. 700 1 $aPÁDUA, S. B. de 700 1 $aALVES, L. de O. 700 1 $aSAKABE, R. 700 1 $aBELO, M. A. de A. 700 1 $aPILARSKI, F. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 5, p. 431-435, maio. 2016.
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