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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
01/03/1999 |
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Data da última atualização: |
01/03/1999 |
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Autoria: |
TAKKAR, P. N.; KAUR, N. P. |
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Afiliação: |
Department of Soils, Punjab Agricultural University, Ludhiana, India. |
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Título: |
HC1 method for Fe2+ estimation to resolve iron chlorosis in plants. |
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Ano de publicação: |
1984 |
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Fonte/Imprenta: |
Journal of Plant Nutrition, v.7, n.1/5, p.81-90, 1984. |
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Idioma: |
Inglês |
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Conteúdo: |
The equation dictates that acid solutions should not cause reduction of Fe3+ during extraction of Fe2+ from plants and provide the possibility to preferentially extract Fe2+. Both in vitro and in vivo extraction of Fe2+ with 1N HCl did not cause reduction of Fe3+. Two g chopped fresh plant was reacted with 0.5 to 1.5N HCl and 1:5 to 1:10 tissue: extractant ratios for 24 h. The Fe2+ and Fe3+ in buffered filterates (pH 3.0) were determined with 1-10 o-phenanthroline (o-Ph). 1N HCl and 1:10 ratio resolved better the Fe2+ differences between green and iron-chlorotic plants. Fe2+ iron in chlorotic as compared to non-chlorotic leaves of different crops was 30 to 82%. This method resolved better or equally good, the visible iron chlorosis in plants, compared to o-Ph method and is much cheaper. The method also eliminates the inherent error arising from extraction of pigments. |
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Palavras-Chave: |
Acid fraction; Active iron; Ferro ativo; Ferroin; Fracao acida; In vivo; Invitro; Iron chlorosis; Reduction. |
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Thesagro: |
Redução. |
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Thesaurus Nal: |
chlorophyll. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01551naa a2200265 a 4500
001 1793802
005 1999-03-01
008 1984 bl --- 0-- u #d
100 1 $aTAKKAR, P. N.
245 $aHC1 method for Fe2+ estimation to resolve iron chlorosis in plants.
260 $c1984
520 $aThe equation dictates that acid solutions should not cause reduction of Fe3+ during extraction of Fe2+ from plants and provide the possibility to preferentially extract Fe2+. Both in vitro and in vivo extraction of Fe2+ with 1N HCl did not cause reduction of Fe3+. Two g chopped fresh plant was reacted with 0.5 to 1.5N HCl and 1:5 to 1:10 tissue: extractant ratios for 24 h. The Fe2+ and Fe3+ in buffered filterates (pH 3.0) were determined with 1-10 o-phenanthroline (o-Ph). 1N HCl and 1:10 ratio resolved better the Fe2+ differences between green and iron-chlorotic plants. Fe2+ iron in chlorotic as compared to non-chlorotic leaves of different crops was 30 to 82%. This method resolved better or equally good, the visible iron chlorosis in plants, compared to o-Ph method and is much cheaper. The method also eliminates the inherent error arising from extraction of pigments.
650 $achlorophyll
650 $aRedução
653 $aAcid fraction
653 $aActive iron
653 $aFerro ativo
653 $aFerroin
653 $aFracao acida
653 $aIn vivo
653 $aInvitro
653 $aIron chlorosis
653 $aReduction
700 1 $aKAUR, N. P.
773 $tJournal of Plant Nutrition$gv.7, n.1/5, p.81-90, 1984.
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Registro original: |
Embrapa Pantanal (CPAP) |
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