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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
05/04/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
MALAFAIA, P.; SALCEDO, Y. T. G.; USCATEGUI, R. A. R.; SOUZA, V. C.; COSTA, D. F. A.; BERCHIELLI, T. T. |
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Afiliação: |
PEDRO MALAFAIA, UFRRJ; YURY T. G. SALCEDO, UNESP; RICARDO A. R. USCATEGUI, UNESP; VINÍCIUS C. SOUZA, UNESP; DIOGO FLEURY A. COSTA, DCA/Centro Universitário Octavio Bastos; TELMA T. BERCHIELLI, UNESP. |
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Título: |
A simple and fast sampling method for chemical analyses na densitometry of bones through rib biopsies in cattle. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 1, p. 31-35, jan. 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
We developed and evaluated a fast and simple method to obtain suitable bone samples for densitometry and chemical analysis through biopsies of the 12th rib of cattle. The postoperative recovery, dry matter intake (DMI) and average daily weight gain (ADG) was evaluated in 36 Nellore steers, nine of which were randomly selected for the control group and 27 others were rib biopsied. Every 30 days, rib biopsy was performed in nine steers, using a corded-electric pistol-grip drill coupled with a hole saw of a 3mm diameter pilot drill bit. This rib biopsy technique provided a suitable sample obtained in a fast way and allowed the surgeon to work alone with the animal slightly sedated and restrained in the crush. Dry matter intake (DMI) was not affected in biopsied animals. At the end of the experimental period (116 days) the average daily weight gain (ADG) was similar in the steers biopsied or not. The described method provided rib samples from cattle suitable for densitometry and chemical analysis of bone tissue without effects on health and performance. This information could greatly increase the accuracy for the diagnosis of phosphorus deficiency in cattle raised on pasture and allow the evaluation of bone metabolism in experimental animals. |
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Palavras-Chave: |
Bone densitometry; Densitometria; Mineral deficiency; Rib biopsy; Sampling method. |
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Thesagro: |
Análise química; Bos indicus; Bovino; Fósforo; Gado nelore; Gado zebu. |
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Thesaurus Nal: |
Bone density; Chemical analysis; Phosphorus; Zebu. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/158657/1/A-simple-and-fast-sampling-methdo-for-chemical.pdf
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Marc: |
LEADER 02274naa a2200361 a 4500
001 2068162
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aMALAFAIA, P.
245 $aA simple and fast sampling method for chemical analyses na densitometry of bones through rib biopsies in cattle.
260 $c2017
520 $aWe developed and evaluated a fast and simple method to obtain suitable bone samples for densitometry and chemical analysis through biopsies of the 12th rib of cattle. The postoperative recovery, dry matter intake (DMI) and average daily weight gain (ADG) was evaluated in 36 Nellore steers, nine of which were randomly selected for the control group and 27 others were rib biopsied. Every 30 days, rib biopsy was performed in nine steers, using a corded-electric pistol-grip drill coupled with a hole saw of a 3mm diameter pilot drill bit. This rib biopsy technique provided a suitable sample obtained in a fast way and allowed the surgeon to work alone with the animal slightly sedated and restrained in the crush. Dry matter intake (DMI) was not affected in biopsied animals. At the end of the experimental period (116 days) the average daily weight gain (ADG) was similar in the steers biopsied or not. The described method provided rib samples from cattle suitable for densitometry and chemical analysis of bone tissue without effects on health and performance. This information could greatly increase the accuracy for the diagnosis of phosphorus deficiency in cattle raised on pasture and allow the evaluation of bone metabolism in experimental animals.
650 $aBone density
650 $aChemical analysis
650 $aPhosphorus
650 $aZebu
650 $aAnálise química
650 $aBos indicus
650 $aBovino
650 $aFósforo
650 $aGado nelore
650 $aGado zebu
653 $aBone densitometry
653 $aDensitometria
653 $aMineral deficiency
653 $aRib biopsy
653 $aSampling method
700 1 $aSALCEDO, Y. T. G.
700 1 $aUSCATEGUI, R. A. R.
700 1 $aSOUZA, V. C.
700 1 $aCOSTA, D. F. A.
700 1 $aBERCHIELLI, T. T.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 1, p. 31-35, jan. 2017.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
28/05/2019 |
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Data da última atualização: |
07/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRILHANTE, N. S.; BIZZO, H. R.; FARIA-MACHADO, A. F. de; SANTOS, M. C. da S.; GAMA, P. E. |
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Afiliação: |
Nathália Simon Brilhante; HUMBERTO RIBEIRO BIZZO, CTAA; ADELIA FERREIRA DE FARIA MACHADO, CTAA; MARCELLY CRISTINA DA SILVA SANTOS, CTAA; PAOLA ERVATTI GAMA, CTAA. |
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Título: |
Method development for the analysis of volatile compounds in olive oil. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: CONGRESSO DE ÓLEOS E GORDURAS, 4., INTERNATIONAL MEETEING ON FATS AND OILS. Campinas, 2019. Anais... Campinas: SBOG, p. 12. |
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Idioma: |
Inglês |
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Notas: |
De 15 a 16 maio 2019. Trabalhos apresentados de forma oral. |
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Conteúdo: |
Olive oil consists mainly of triglycerides, in about 97 to 99% by weight. The minor compounds are a complex mixture of polar, apolar and amphiphilic substances, such as tocopherols, phenolic compounds, sterols, chlorophyll, carotenoids, terpene acids, monoglycerides and diglycerides, free fatty acids and volatile compounds. These volatiles are the compounds directly responsible for the aroma of the oil. Extra virgin olive oil has a complex aroma with more than 100 volatile compounds identified, among aldehydes, alcohols, esters, hydrocarbons, ketones and furans. The objective of this study was to develop an analytical method for volatile compounds in olive oils using solid-phase microextraction (SPME), gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detection (GC-FID). For the SPME, different parameters like flask size (4, 10 and 20 mL), sampling temperatures (40 and 60 °C), headspace conditioning (10 and 60 min) and fiber exposure times (15 and 40 min) were tested. For GC analyses two different internal standards, methyl octanoate and tetradecane, were tested, as well as sub-ambient oven temperatures with liquid nitrogen. A 1 g of sample and a divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PMDS) fiber were used in all the tests. Analytical curves from the FID data were constructed for linearity evaluation, whereas internal standard was used for quantification ofcompounds such as 3-hexenol, 2-hexanal and limonene. Identification was performed based on mass spectra, co-injection of standards and retention indices data. The best conditions for SPME analysis were sample temperature of 40 °C, 10 min of headspace conditioning and fiber exposure for 40 min, in a 4 mL flask. For the chromatographic analyzes, tetradecane was chosen as the internal standard. Oven temperature program with cryofocusing led to a much better separation and, therefore, better quantitation and identification of the morevolatile compounds. MenosOlive oil consists mainly of triglycerides, in about 97 to 99% by weight. The minor compounds are a complex mixture of polar, apolar and amphiphilic substances, such as tocopherols, phenolic compounds, sterols, chlorophyll, carotenoids, terpene acids, monoglycerides and diglycerides, free fatty acids and volatile compounds. These volatiles are the compounds directly responsible for the aroma of the oil. Extra virgin olive oil has a complex aroma with more than 100 volatile compounds identified, among aldehydes, alcohols, esters, hydrocarbons, ketones and furans. The objective of this study was to develop an analytical method for volatile compounds in olive oils using solid-phase microextraction (SPME), gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detection (GC-FID). For the SPME, different parameters like flask size (4, 10 and 20 mL), sampling temperatures (40 and 60 °C), headspace conditioning (10 and 60 min) and fiber exposure times (15 and 40 min) were tested. For GC analyses two different internal standards, methyl octanoate and tetradecane, were tested, as well as sub-ambient oven temperatures with liquid nitrogen. A 1 g of sample and a divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PMDS) fiber were used in all the tests. Analytical curves from the FID data were constructed for linearity evaluation, whereas internal standard was used for quantification ofcompounds such as 3-hexenol, 2-hexanal and limonene. I... Mostrar Tudo |
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Palavras-Chave: |
GC-FID; GC-MS; SPME. |
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Thesaurus NAL: |
Olive oil; Volatile compounds. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/197804/1/resumo-4COG-p.12.pdf
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Marc: |
LEADER 02772nam a2200229 a 4500
001 2109353
005 2020-02-07
008 2019 bl uuuu u00u1 u #d
100 1 $aBRILHANTE, N. S.
245 $aMethod development for the analysis of volatile compounds in olive oil.$h[electronic resource]
260 $aIn: CONGRESSO DE ÓLEOS E GORDURAS, 4., INTERNATIONAL MEETEING ON FATS AND OILS. Campinas, 2019. Anais... Campinas: SBOG, p. 12.$c2019
500 $aDe 15 a 16 maio 2019. Trabalhos apresentados de forma oral.
520 $aOlive oil consists mainly of triglycerides, in about 97 to 99% by weight. The minor compounds are a complex mixture of polar, apolar and amphiphilic substances, such as tocopherols, phenolic compounds, sterols, chlorophyll, carotenoids, terpene acids, monoglycerides and diglycerides, free fatty acids and volatile compounds. These volatiles are the compounds directly responsible for the aroma of the oil. Extra virgin olive oil has a complex aroma with more than 100 volatile compounds identified, among aldehydes, alcohols, esters, hydrocarbons, ketones and furans. The objective of this study was to develop an analytical method for volatile compounds in olive oils using solid-phase microextraction (SPME), gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detection (GC-FID). For the SPME, different parameters like flask size (4, 10 and 20 mL), sampling temperatures (40 and 60 °C), headspace conditioning (10 and 60 min) and fiber exposure times (15 and 40 min) were tested. For GC analyses two different internal standards, methyl octanoate and tetradecane, were tested, as well as sub-ambient oven temperatures with liquid nitrogen. A 1 g of sample and a divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PMDS) fiber were used in all the tests. Analytical curves from the FID data were constructed for linearity evaluation, whereas internal standard was used for quantification ofcompounds such as 3-hexenol, 2-hexanal and limonene. Identification was performed based on mass spectra, co-injection of standards and retention indices data. The best conditions for SPME analysis were sample temperature of 40 °C, 10 min of headspace conditioning and fiber exposure for 40 min, in a 4 mL flask. For the chromatographic analyzes, tetradecane was chosen as the internal standard. Oven temperature program with cryofocusing led to a much better separation and, therefore, better quantitation and identification of the morevolatile compounds.
650 $aOlive oil
650 $aVolatile compounds
653 $aGC-FID
653 $aGC-MS
653 $aSPME
700 1 $aBIZZO, H. R.
700 1 $aFARIA-MACHADO, A. F. de
700 1 $aSANTOS, M. C. da S.
700 1 $aGAMA, P. E.
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Embrapa Agroindústria de Alimentos (CTAA) |
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