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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
02/06/2000 |
Data da última atualização: |
07/03/2013 |
Autoria: |
MEZENCIO, J. M. S.; MOUSSATCHE, N.; MOSCARDI, F.; REBELLO, M. A. |
Título: |
DNA-dependent RNA polymerase activity associated with Anticarsia gemmatalis nuclear polyhedrosis virus. |
Ano de publicação: |
1986 |
Fonte/Imprenta: |
In: ENCONTRO NACIONAL DE VIROLOGIA, 3., 1986, Sao Lourenco. Resumos... Rio de Janeiro: Centro Panamericano de Febre Aftosa, 1986. p.103. |
Idioma: |
Inglês |
Conteúdo: |
Anticarsia gemmatalis nuclear polyhedrosis virus (Ag-NPV) is an insect patogen, member of the Baculoviridae family. This group of virus contains a large, circular double-strand DNA genome at 7,5-10 x 10 daltons, that replicates in the nuclei of infected host cells. The infection leads to the formation of polyhedral inclusion bodies referred polyhedra (Summers & Smith, Virology, 84:390-402, 1978). Studies on the replication of baculovirus has been demonstrated the induction of DNA-dependent DNA polymerase (Miller et al., J. Virol., 40:305-308, 1981) and alpha-amanitin resistant DNA-dependent RNA polymerase (Fucns et al., J. Virol., 48:641-646, 1983) by Autographa californica nuclear polyedrosis virus in lepidoptera infected cells. This results suggest the presence of a viral RNA polymerase codified by the viral genone. In this communication we report a possible DNA-dependent RNA polymerase associated with Ag-NPV polyhedra. The enzyme activity was measured after activation the mixture contained polyhedra with sarkosyl or alpha-chymotrypsin. The enzyme was resistant to alpha-amanitin, and the addition of rifampicin, actinomycin D. ethidium bromide or neutral acriflavin, inhibit the transcription activity to some extent. The transcription products analysed by sucrose gradient centrifugation shows the presence of 3 RNA peaks. This results suggest a possible presence of RNA polymerase associated with Ag-NPV polyhedra. |
Palavras-Chave: |
Anticarsia germmatalis; Brasil; Insects pest; Insetos pragas; Nuclear polyhedrosis virus; Poliedrose nuclear. |
Thesagro: |
Controle Biológico; Vírus. |
Thesaurus Nal: |
biological control; Brazil. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02312naa a2200277 a 4500 001 1461949 005 2013-03-07 008 1986 bl --- 0-- u #d 100 1 $aMEZENCIO, J. M. S. 245 $aDNA-dependent RNA polymerase activity associated with Anticarsia gemmatalis nuclear polyhedrosis virus. 260 $c1986 520 $aAnticarsia gemmatalis nuclear polyhedrosis virus (Ag-NPV) is an insect patogen, member of the Baculoviridae family. This group of virus contains a large, circular double-strand DNA genome at 7,5-10 x 10 daltons, that replicates in the nuclei of infected host cells. The infection leads to the formation of polyhedral inclusion bodies referred polyhedra (Summers & Smith, Virology, 84:390-402, 1978). Studies on the replication of baculovirus has been demonstrated the induction of DNA-dependent DNA polymerase (Miller et al., J. Virol., 40:305-308, 1981) and alpha-amanitin resistant DNA-dependent RNA polymerase (Fucns et al., J. Virol., 48:641-646, 1983) by Autographa californica nuclear polyedrosis virus in lepidoptera infected cells. This results suggest the presence of a viral RNA polymerase codified by the viral genone. In this communication we report a possible DNA-dependent RNA polymerase associated with Ag-NPV polyhedra. The enzyme activity was measured after activation the mixture contained polyhedra with sarkosyl or alpha-chymotrypsin. The enzyme was resistant to alpha-amanitin, and the addition of rifampicin, actinomycin D. ethidium bromide or neutral acriflavin, inhibit the transcription activity to some extent. The transcription products analysed by sucrose gradient centrifugation shows the presence of 3 RNA peaks. This results suggest a possible presence of RNA polymerase associated with Ag-NPV polyhedra. 650 $abiological control 650 $aBrazil 650 $aControle Biológico 650 $aVírus 653 $aAnticarsia germmatalis 653 $aBrasil 653 $aInsects pest 653 $aInsetos pragas 653 $aNuclear polyhedrosis virus 653 $aPoliedrose nuclear 700 1 $aMOUSSATCHE, N. 700 1 $aMOSCARDI, F. 700 1 $aREBELLO, M. A. 773 $tIn: ENCONTRO NACIONAL DE VIROLOGIA, 3., 1986, Sao Lourenco. Resumos... Rio de Janeiro: Centro Panamericano de Febre Aftosa, 1986. p.103.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
13/06/2022 |
Data da última atualização: |
13/06/2022 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
SANCHES, N. F.; FANCELLI, M.; NASCIMENTO, A. S. do. |
Afiliação: |
NILTON FRITZONS SANCHES, CNPMF; MARILENE FANCELLI, CNPMF; ANTONIO SOUZA DO NASCIMENTO, CNPMF. |
Título: |
Pragas e seu controle. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
In: OLIVEIRA, A. M. G.; MEISSNER FILHO, P. E. Mamoeiro do grupo solo: cultivo, colheita, pós-colheita e comercialização. Brasília, DF : Embrapa, 2022. Cap. 11. p. 191-229. |
Idioma: |
Português |
Thesagro: |
Mamão; Praga de Planta. |
Thesaurus NAL: |
Papayas. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144018/1/Capitulo11-MamaoGrupoSolo-Nilton-Marilene-AntonioNascimento-AINFO.pdf
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Marc: |
LEADER 00573naa a2200169 a 4500 001 2144018 005 2022-06-13 008 2022 bl uuuu u00u1 u #d 100 1 $aSANCHES, N. F. 245 $aPragas e seu controle.$h[electronic resource] 260 $c2022 650 $aPapayas 650 $aMamão 650 $aPraga de Planta 700 1 $aFANCELLI, M. 700 1 $aNASCIMENTO, A. S. do 773 $tIn: OLIVEIRA, A. M. G.; MEISSNER FILHO, P. E. Mamoeiro do grupo solo: cultivo, colheita, pós-colheita e comercialização. Brasília, DF : Embrapa, 2022. Cap. 11. p. 191-229.
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